A host-mediated in vivo/in vitro assay with peritoneal murine macrophages for the detection of carcinogenic chemicals

Massa, Th.; Gerber, Teresa; Pfaffenholz, V.; Chandra, Angelika; Schlatterer, B; Prakash, Chandra

doi:10.1007/bf01612918

Cited by 9 publications

(7 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Exposure to chemicals, such as alpha-naphthylamine and N-methyl-N-nitrosourea, can render macrophages themselves tumorigenic. Indeed, peritoneal murine macrophages exposed to these chemicals can give rise to immortal cell lines with tumorigenic activity in athymic nu/nu mice (112). But other agents, besides chemical ones, can also promote the tumorigenic activity of macrophages.…”

Section: Carcinogens and Activation Of The Innate Immune Systemmentioning

confidence: 99%

The effect of environmental chemicals on the tumor microenvironment

Casey¹,

Vaccari²,

Al-Mulla

et al. 2015

CARCIN

114

View full text Add to dashboard Cite

Potentially carcinogenic compounds may cause cancer through direct DNA damage or through indirect cellular or physiological effects. To study possible carcinogens, the fields of endocrinology, genetics, epigenetics, medicine, environmental health, toxicology, pharmacology and oncology must be considered. Disruptive chemicals may also contribute to multiple stages of tumor development through effects on the tumor microenvironment. In turn, the tumor microenvironment consists of a complex interaction among blood vessels that feed the tumor, the extracellular matrix that provides structural and biochemical support, signaling molecules that send messages and soluble factors such as cytokines. The tumor microenvironment also consists of many host cellular effectors including multipotent stromal cells/mesenchymal stem cells, fibroblasts, endothelial cell precursors, antigen-presenting cells, lymphocytes and innate immune cells. Carcinogens can influence the tumor microenvironment through effects on epithelial cells, the most common origin of cancer, as well as on stromal cells, extracellular matrix components and immune cells. Here, we review how environmental exposures can perturb the tumor microenvironment. We suggest a role for disrupting chemicals such as nickel chloride, Bisphenol A, butyltins, methylmercury and paraquat as well as more traditional carcinogens, such as radiation, and pharmaceuticals, such as diabetes medications, in the disruption of the tumor microenvironment. Further studies interrogating the role of chemicals and their mixtures in dose-dependent effects on the tumor microenvironment could have important general mechanistic implications for the etiology and prevention of tumorigenesis.

show abstract

Section: Carcinogens and Activation Of The Innate Immune Systemmentioning

confidence: 99%

The effect of environmental chemicals on the tumor microenvironment

Casey¹,

Vaccari²,

Al-Mulla

et al. 2015

CARCIN

114

View full text Add to dashboard Cite

show abstract

“…Normal peritoneal macrophages were isolated from NMRI-mice after intraperitoneal injection of LPS [13]. Both cell types were maintained in DMEM containing 10% fetal calf serum (FCS) and 1% stock solution of penicillin/streptomycin in a humidified incubator at 37°C and an atmosphere of 5% CO,.…”

Section: Cell Culturementioning

confidence: 99%

Low molecular weight proteins secreted by peritoneal macrophages obtained from 2,3,7,8‐tetrabromodibenzo‐p‐dioxin‐treated NMRI mice

et al. 1997

View full text Add to dashboard Cite

The protein secretion patterns in a macrophage-like cell line (CBrD), established from the peritoneal cells of NMRI mice treated with the dioxin analog 2,3,7,8-tetrabromodibenzo-p-dioxin (TBrDD), were analyzed by high resolution two-dimensional gel electrophoresis (2-D PAGE), and compared to the pattern of proteins secreted by control macrophages which were intraperitoneally activated by bacterial lipopolysaccharide. The most striking alterations were observed in the low molecular range. The transformed cells encode a number of low molecular mass proteins (10-20 kDa) which were not detected in control cells under identical experimental conditions. The protein pattern with respect to isoelectric point, molecular weight, optical density (OD) and area of the spot (in mm2) has been depicted by computer analysis in relation to a standardized spot outline and the spot's background (in OD). It is concluded that the transformation of murine peritoneal macrophages by TBrDD leads to an upregulation of proteins, in particular of low-molecular-weight proteins.

show abstract

“…This review focuses on a host-mediated in vivo/in vitro model assay system developed by our group (9). A major advantage of host-mediated models compared to solely in vitro studies is that they allow researchers to monitor the biotransformation of chemical mutagens through metabolic activation or detoxification and elimination.…”

mentioning

confidence: 99%

“…In this review, the ability of our in vivo/in vitro assay system (9) to detect the transforming potential of chemicals was compared to that of established genotoxicity and mutagenicity test systems, such as the Ames test ( 6), micronucleus assay (10) and Comet assay (7,11). Additionally, a comparison of literature carcinogenicity data and current classifications by the IARC with respect to carcinogenic potential was made.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Applicability of a Host-mediatedIn Vivo/In VitroModel in Screening for the Carcinogenic Potential of Chemicals

et al. 2022

View full text Add to dashboard Cite

Based on a publication of Tomasetti and Vogelstein in 2015, in which the risk of cancer development is postulated to be just one-third caused by genetic predisposition and environmental factors, it seemed worth focusing again on the value of test systems for screening chemicals for their carcinogenicity. This review aims to firstly summarize data on a host-mediated in vivo/in vitro assay developed by our working group to screen the tumorigenic potential of chemicals. Subsequently, in this article the importance and advantages of host-mediated in vivo/in vitro assays in general have been compared with in vivo and in vitro tests. The applicability of the host-mediated in vivo/in vitro assay system within broad screening strategies is discussed. The main intention of this review is to stimulate developments of newer approaches in the field of carcinogenic testing.

show abstract

A host-mediated in vivo/in vitro assay with peritoneal murine macrophages for the detection of carcinogenic chemicals

Cited by 9 publications

References 21 publications

The effect of environmental chemicals on the tumor microenvironment

The effect of environmental chemicals on the tumor microenvironment

Low molecular weight proteins secreted by peritoneal macrophages obtained from 2,3,7,8‐tetrabromodibenzo‐p‐dioxin‐treated NMRI mice

Applicability of a Host-mediatedIn Vivo/In VitroModel in Screening for the Carcinogenic Potential of Chemicals

Contact Info

Product

Resources

About