A Hospital-based Case-control Study of Diarrhea in Children in Shanghai

Chang, Hailing; Zhang, Ling; Ge, Yigong; Cai, Jiehao; Wang, Xiangshi; Huang, Zheng; Guo, Jiayin; Xu, Hao; Gu, Zhen; Chen, Hong; Xu, Xuebin; Zeng, Mei

doi:10.1097/inf.0000000000001562

Cited by 18 publications

(21 citation statements)

References 32 publications

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“…Although most of the NTS in this study were isolated from patients 5-years-ofage, a considerable number of patients were 5-yearsof-age. A similar trend has been reported in other Asian countries (11)(12)(13)(14).…”

Section: Discussionsupporting

confidence: 89%

“…We found that majority of the NTS isolates were MDR; this trend has been recently evident in Delhi and neighboring regions. NTS isolated in India and other countries are generally susceptible to many antimicrobials (12,13,37). Except for S. Kentucky, the other NTS isolates from diarrheal patients from 2011 to 2014 in Manipal, south of India, were susceptible to most of the tested antimicrobials (38).…”

Section: Discussionmentioning

confidence: 99%

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Prevalence of Multidrug Resistant Salmonellae with Increasing Frequency of <i>Salmonella enterica</i> Serovars Kentucky and Virchow among Hospitalized Diarrheal Cases in and around Delhi, India

Sharma¹,

Kumar

Sarkar

et al. 2020

Jpn J Infect Dis

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Section: Discussionsupporting

confidence: 89%

Section: Discussionmentioning

confidence: 99%

Prevalence of Multidrug Resistant Salmonellae with Increasing Frequency of <i>Salmonella enterica</i> Serovars Kentucky and Virchow among Hospitalized Diarrheal Cases in and around Delhi, India

Sharma¹,

Kumar

Sarkar

et al. 2020

Jpn J Infect Dis

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“…DEC was detected in 12.7% of children with acute diarrhea in the study and most DEC isolates were EAEC and EPEC, which is consistent with studies conducted in Israel (34). However, our previous casecontrol study conducted in 2014 didn't demonstrate the pathogenic significance of EAEC and EPEC (8). Therefore, we are uncertain the role of EAEC and EPEC as an etiological agent of infectious diarrhea in Shanghainese children.…”

Section: Discussionsupporting

confidence: 85%

“…norovirus Group I and Group II, adenovirus, sapovirus and astrovirus. Bacteria pathogens were identified as described in our previous study (8). Viruses were identified based on multiplex quantitative Real-time PCR using BioPerfectus kits (Jiangsu BioPerfectus Technologies Co., Ltd., Jiangsu, China).…”

Section: Sample Collection and Laboratory Methodsmentioning

confidence: 99%

Etiology of acute diarrhea in children in Shanghai, 2015-2018

Chang

Guo

Wei

et al. 2019

Preprint

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Background Diarrhea is still a major cause of childhood morbidity and mortality worldwide. This hospital-based study aimed to monitor the consecutive epidemiological trend of etiology in children with acute diarrhea in Shanghai.Methods Outpatient children with diarrhea were prospectively enrolled within 7 days after onset of diarrheal symptoms during 2015-2018. Fresh stool samples were collected for testing enteropathogens. Enteric bacteria were identified and typed through culture and serotyping. Enteric viruses were identified through real-time PCR assay. hadResults Enteric pathogens were identified in 1572 (58.4%) of the 2692 enrolled children with acute diarrhea. Viruses were more frequently detected than bacteria (41.3% versus 25.0%), and co-infection with 2 or more pathogens was found in 13% of outpatients. Nontyphoidal Salmonella spp . (NTS) was the most common bacteria with 10.3% of isolate rate, followed by enteropathogenic Escherichia coli (EPEC) (6.5%), enteroaggregative Escherichia coli (EAEC) (6.2%), Campylobacter spp . (3.6%), enterotoxigenic Escherichia coli (ETEC) (1.1%), Shigella spp . (0.2%), and enterohemorrhagic Escherichia coli (EHEC) (0.1%). Rotavirus was the most common virus with 16.0% of detection rate, followed by norovirus (15.5%), adenovirus (7.2%), sapovirus (3.0%) and astrovirus (2.7%).Conclusions Infectious diarrhea remains the major cause of diarrhea in children in Shanghai. Rotavirus, norovirus and NTS were the major enteric pathogens responsible for diarrhea in Shanghainese children. Improving uptake of rotavirus vaccine and strengthening prevention of foodborne pathogens will be helpful to reduce the burden of diarrheal diseases in children in Shanghai.

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“…In China, Campylobacter infections are not subject to mandatory notification and Campylobacter surveillance is on a voluntary basis by local and regional laboratories. In part due to insensitive conventional isolation methods, there were not many cases of Campylobacter infection detected by monitoring since 2000 [10,11]. Although recently, a modified membrane filtration culture, has led to increased identification of a broad range of Campylobacter [12], there have been no outbreak reports so far.…”

Section: Introductionmentioning

confidence: 99%

Molecular and epidemiologyical analysis of a Campylobacter jejuni outbreak in China, 2018

Zhang

et al. 2019

J Infect Dev Ctries

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Introduction: Campylobacter spp. is the most common gastrointestinal pathogen worldwide with a very low reported incidence in China. In April 2018, one 36 cases of diarrhea outbreak occurred in a high school in Beijing after a trip to another province in Southern China. The investigation for the enteric pathogen infection was conducted to identify the cause. Methodology: Eighteen stool specimens from 11 diarrheal patients and 3 close contacts were collected and tested for 16 enteric bacterial and viral pathogens using real-time PCR methods. Multilocus Sequence Typing (MLST), pulsed-field gel electrophoresis (PFGE) and antimicrobial susceptibility testing were applied to C. jejuni isolates in this outbreak in order to characterize the incident. Results: Ten (90.9%) of 11 stool specimens from diarrheal patients were positive for Campylobacter jejuni, confirmed as the cause of outbreak. Eight C. jejuni strains were obtained and produced 2 sequence types (STs) and 3 PFGE patterns. Six of them had the same ST (ST2274) and PFGE pattern (SMA001). The dominant outbreak strain with an identical subtyping profile was clustered with most chicken isolates from national MLST and PulseNet Campylobacter database. The entire 8 isolates were multi-drug resistant, with the dominant resistance pattern of nalidixic acid, tetracycline and ciprofloxacin combined, except for 2 isolates resistant to florfenicol. Conclusion: Molecular typing confirmed that most of the cases belonged to a clonal cluster supporting the hypothesis of a common source; however, the source was not identified. This was the first recognized Campylobacteriosis outbreak in China among 20 years.

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A Hospital-based Case-control Study of Diarrhea in Children in Shanghai

Cited by 18 publications

References 32 publications

Prevalence of Multidrug Resistant Salmonellae with Increasing Frequency of <i>Salmonella enterica</i> Serovars Kentucky and Virchow among Hospitalized Diarrheal Cases in and around Delhi, India

Prevalence of Multidrug Resistant Salmonellae with Increasing Frequency of <i>Salmonella enterica</i> Serovars Kentucky and Virchow among Hospitalized Diarrheal Cases in and around Delhi, India

Etiology of acute diarrhea in children in Shanghai, 2015-2018

Molecular and epidemiologyical analysis of a Campylobacter jejuni outbreak in China, 2018

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