Abstract:We present a checklist of 278 species of reef fishes recorded along the coastline of Santa Catarina state, the southernmost limit of distribution of tropical ichthyofauna on the coast of Brazil. regius are reported for the first time, respectively, from the Southwestern Atlantic and for the coastal part of this region, while Acanthurus monroviae is reported for the second time for the Southwestern Atlantic. We present habitat distribution, trophic structure and comment on biogeographic affinities of this transitional region, discussing both remarkable species presences and absences.