A homotetrameric agglutinin with antiproliferative and mitogenic activities from haricot beans

Wong, Jack Ho; Ng, T.B.

doi:10.1016/j.jchromb.2005.09.044

Cited by 13 publications

(4 citation statements)

References 25 publications

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“…Anasazi Bean lectin ; HBL, P. vulgaris cv. Haricot Bean lectin ; FBH12, lectin from P. vulgaris cv. French Bean cultivar number 12 ; FBH35, lectin from P. vulgaris cv.…”

Section: Resultsmentioning

confidence: 99%

A Lectin with Anti-HIV-1 Reverse Transcriptase, Antitumor, and Nitric Oxide Inducing Activities from Seeds of Phaseolus vulgaris cv. Extralong Autumn Purple Bean

Fang

Lin

Wong

et al. 2010

J. Agric. Food Chem.

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Lectins/hemagglutinins are a class of sugar-binding proteins which agglutinate cells and/or precipitate glycoconjugates. They occur widely in plants but manifest significant differences in activities, which means only a few of them own exploitable potentials. The objective of this study was to find and characterize a multifunctional plant lectin with high potential values in food chemistry and medicine. A 60-kDa lectin from Phaseolus vulgaris L. cv. Extralong Autumn Purple Bean (EAPL) was purified by liquid chromatography, and the sequence of its first 20 N-terminal amino acids was ANEIYFSFQRFNETNLILQR. It was galactose-specific and manifested hemagglutinating activity toward erythrocytes of rabbit, rat, mouse, and human ABO blood types. EAPL manifested anti-HIV-1-RT activity, and it could inhibit the proliferation of human tumor cells by inducing the production of apoptotic bodies. The nitric oxide-inducing activity of EAPL may find application in tumor therapy.

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“…Anasazi Bean lectin ; HBL, P. vulgaris cv. Haricot Bean lectin ; FBH12, lectin from P. vulgaris cv. French Bean cultivar number 12 ; FBH35, lectin from P. vulgaris cv.…”

Section: Resultsmentioning

confidence: 99%

A Lectin with Anti-HIV-1 Reverse Transcriptase, Antitumor, and Nitric Oxide Inducing Activities from Seeds of Phaseolus vulgaris cv. Extralong Autumn Purple Bean

Fang

Lin

Wong

et al. 2010

J. Agric. Food Chem.

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“…Interestingly, some activity remained even after heating at 100°C for 30 minutes. Haricot bean and ground bean lectins, however, have been shown to lose activity beyond 40°C in a temperature-dependent manner [ 19 , 36 ]. The lectin shows remarkable pH stability, its activity being unaffected throughout the entire range of pH from 1 to 14.…”

Section: Discussionmentioning

confidence: 99%

Purification and Characterization of a Lectin from Phaseolus vulgaris cv. (Anasazi Beans)

Sharma

Wong

et al. 2009

BioMed Research International

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A lectin has been isolated from seeds of the Phaseolus vulgaris cv. “Anasazi beans” using a procedure that involved affinity chromatography on Affi-gel blue gel, fast protein liquid chromatography (FPLC)-ion exchange chromatography on Mono S, and FPLC-gel filtration on Superdex 200. The lectin was comprised of two 30-kDa subunits with substantial N-terminal sequence similarity to other Phaseolus lectins. The hemagglutinating activity of the lectin was stable within the pH range of 1–14 and the temperature range of 0–80°C. The lectin potently suppressed proliferation of MCF-7 (breast cancer) cells with an IC50 of 1.3 μM, and inhibited the activity of HIV-1 reverse transcriptase with an IC50 of 7.6 μM. The lectin evoked a mitogenic response from murine splenocytes as evidenced by an increase in [3H-methyl]-thymidine incorporation. The lectin had no antifungal activity. It did not stimulate nitric oxide production by murine peritoneal macrophages. Chemical modification results indicated that tryptophan was crucial for the hemagglutinating activity of the lectin.

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“…Like straw mushroom lectin, 56 Ganoderma capense lectin, 41 tuber lectin from a wild cobra lily, Arisaema flavum, 57 lectin from tubers of Voodoo lily (Sauromatum venosum) 58 pinto bean lectin, 59 haricot bean lectin, 60 scyllin-2 is endowed with a potent mitogenic effect towards mouse splenocytes. It also exhibited potent inhibitory effect on the proliferation of HepG2 cell lines.…”

Section: Discussionmentioning

confidence: 99%

Tn/T Specific Agglutinin from Estuarine Crab Scylla Serrata with Potent Mitogenic Activity on Mouse Splenocytes and Antiproliferative Effect on Hepatocellular Carcinoma (HepG2) Cell

Pramanik¹,

Chatterjee²,

Mondal³

et al. 2010

Glycobiology Insights

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A lectin was purified from the hemolymph of esturarine crab Scylla serrata by successive 40% (NH 4) 2 SO 4 precipitation, affinity chromatography on asialo fetuin-Sepharose column, Resource Q anion-exchanger in FPLC system and designated as scyllin-2. Scyllin-2 was a homogeneous monomeric protein of molecular mass 75 kD judged by SDS-PAGE and confirmed by ESI-MS-Q-ToF. Its activity was Ca +2 dependent being maximum at pH 7.5 and at 20 °C. N-terminal sequence of scyllin-2 showed close resemblance to peanut lectin and histidine kinase A. It agglutinated human O, A, B and AB blood group erythrocytes equally well and showed maximum inhibition with α-Gal by hapten-inhibition study. The detailed carbohydrate specificity of scyllin-2 was determined at the macromolecular level based on the Gal/GalNAc structural units in the mammalian glycoproteins by enzyme-linked lectinosorbent (ELLSA) and inhibition assays. It revealed that scyllin-2 binds specifically to tumor-associated carbohydrate antigens GalNAcα1→Ser/Thr (Tn) and Galβ1→3GalNAcα1→Ser/Thr (Tα). It showed very weak binding with Galβ1→3/4GlcNAc (I/II) glycotopes on glycoproteins and T/Tn covered by sialic acid. Multivalancy of Tn/Tα containing glycoproteins tested resulted in higher binding of 10 2-10 4 order than the respective Gal and GalNAc monomer. Scyllin-2 stimulated proliferation of mouse splenocytes. Analysis of expression of receptors for scyllin-2 on HepG2 by flow cytometry showed the binding of FITC-scyllin-2 to HepG2 was 86.51%, which was nearly comparable to Artocarpus lakoocha agglutinin (ALA) (66.41%), another Tn/Tα specific lectin indicating that the glycan structure on HepG2 cell surface shows prevalence of Tn/Tα units. It inhibited proliferation of HepG2 cells (61 µg/ml). The inhibitory effect was comparable to ALA (80 µg/ml). Thus, we have characterized a Tn/T specific invertebrate lectin with biological significance.

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A homotetrameric agglutinin with antiproliferative and mitogenic activities from haricot beans

Cited by 13 publications

References 25 publications

A Lectin with Anti-HIV-1 Reverse Transcriptase, Antitumor, and Nitric Oxide Inducing Activities from Seeds of Phaseolus vulgaris cv. Extralong Autumn Purple Bean

A Lectin with Anti-HIV-1 Reverse Transcriptase, Antitumor, and Nitric Oxide Inducing Activities from Seeds of Phaseolus vulgaris cv. Extralong Autumn Purple Bean

Purification and Characterization of a Lectin from Phaseolus vulgaris cv. (Anasazi Beans)

Tn/T Specific Agglutinin from Estuarine Crab Scylla Serrata with Potent Mitogenic Activity on Mouse Splenocytes and Antiproliferative Effect on Hepatocellular Carcinoma (HepG2) Cell

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