A homologue of the breast cancer-associated gene BARD1 is involved in DNA repair in plants

Reidt, Wim; Wurz, Rebecca; Wanieck, Kristina; Chu, Ha Hoang; Puchta, Holger

doi:10.1038/sj.emboj.7601547

Cited by 4 publications

(6 citation statements)

References 28 publications

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“…RBR silencing triggers AtBRCA1 recruitment to DNA damage foci and AtBRCA1 co-localises with RBR foci upon DNA stress BRCA1 is a pivotal DNA repair protein of double-strand DNA damage both in mammals (Rosen, 2013) and in Arabidopsis (Block-Schmidt et al, 2011; Trapp et al, 2011). Atbrca1-1 (Reidt et al, 2006) and Atbrca1-3 loss of function mutants displayed hypersensitive cell death response to genotoxic stress (MMC treatment) compared to Col-0 (Figs 4A and EV3A-E). We generated a genomic AtBRCA1-GFP construct driven by the endogenous promoter (AtBRCA1-GFP) (Appendix Supplementary Methods) and transformed it into the Atbrca1-1 line.…”

Section: Dna Stress Recruits Rbr To Ch2ax-labelled Heterochromatic Focimentioning

confidence: 99%

“…Arabidopsis thaliana ecotype Columbia 0 (Col-0) was used as wild type; T-DNA insertion lines Atbrca1-3 (SALK_099751) and e2fb-2 (SALK_120959) were obtained from the Nottingham Arabidopsis Stock Centre. The transgenic lines sog1-1 (Yoshiyama et al, 2009), e2fa-1, e2fa-2 and e2fb-1 (MPIZ_244, GABI-348E09, SALK_103138, respectively (Berckmans et al, 2011b) (Berckmans et al, 2011a)), e2fa-3 (Xiong et al, 2013), Atbrca1-1 (Reidt et al, 2006), rRBr (Wildwater et al, 2005) and amiRBR (Cruz-Ramirez et al, 2012) were described earlier. The T-DNA insertions and mutations were confirmed by PCR-based genotyping or sequencing and gene silencing was demonstrated via gene expressional studies and phenotyping.…”

Section: Plant Materials and Growth Conditionsmentioning

confidence: 99%

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Arabidopsis RETINOBLASTOMA RELATED directly regulates DNA damage responses through functions beyond cell cycle control

et al. 2017

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The rapidly proliferating cells in plant meristems must be protected from genome damage. Here, we show that the regulatory role of the Arabidopsis RETINOBLASTOMA RELATED (RBR) in cell proliferation can be separated from a novel function in safeguarding genome integrity. Upon DNA damage, RBR and its binding partner E2FA are recruited to heterochromatic γH2AX‐labelled DNA damage foci in an ATM‐ and ATR‐dependent manner. These γH2AX‐labelled DNA lesions are more dispersedly occupied by the conserved repair protein, AtBRCA1, which can also co‐localise with RBR foci. RBR and AtBRCA1 physically interact in vitro and in planta. Genetic interaction between the RBR‐silenced amiRBR and Atbrca1 mutants suggests that RBR and AtBRCA1 may function together in maintaining genome integrity. Together with E2FA, RBR is directly involved in the transcriptional DNA damage response as well as in the cell death pathway that is independent of SOG1, the plant functional analogue of p53. Thus, plant homologs and analogues of major mammalian tumour suppressor proteins form a regulatory network that coordinates cell proliferation with cell and genome integrity.

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Section: Dna Stress Recruits Rbr To Ch2ax-labelled Heterochromatic Focimentioning

confidence: 99%

Section: Plant Materials and Growth Conditionsmentioning

confidence: 99%

Arabidopsis RETINOBLASTOMA RELATED directly regulates DNA damage responses through functions beyond cell cycle control

et al. 2017

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“…Surprisingly, BRCA1 ⁄ 2 homologs have been identified not only in animals but also in plants. In addition to a BRCA1 homolog (Lafarge & Montane, 2003;Reidt et al, 2006;Block-Schmidt et al, 2011), the Arabidopsis genome also contains two copies of the BRCA2 gene, which share 96.8% sequence identity, except for the presence of a 450 bp insertion in an intron of AtBRCA2A (Siaud et al, 2004). The proteins encoded by the two AtBRCA2 genes are 1151 and 1155 amino acids long and are 94.5% identical to each other and 21% identical to the human protein.…”

Section: Introductionmentioning

confidence: 99%

BRCA2 is a mediator of RAD51‐ and DMC1‐facilitated homologous recombination inArabidopsis thaliana

et al. 2011

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Summary• Mutations in the breast cancer susceptibility gene 2 (BRCA2) are correlated with hereditary breast cancer in humans. Studies have revealed that mammalian BRCA2 plays crucial roles in DNA repair. Therefore, we wished to define the role of the BRCA2 homologs in Arabidopsis in detail.• As Arabidopsis contains two functional BRCA2 homologs, an Atbrca2 double mutant was generated and analyzed with respect to hypersensitivity to genotoxic agents and recombination frequencies. Cytological studies addressing male and female meiosis were also conducted, and immunolocalization was performed in male meiotic prophase I.• The Atbrca2 double mutant showed hypersensitivity to the cross-linking agent mitomycin C and displayed a dramatic reduction in somatic homologous recombination frequency, especially after double-strand break induction. The loss of AtBRCA2 also led to severe defects in male meiosis and development of the female gametophyte and impeded proper localization of the synaptonemal complex protein AtZYP1 and the recombinases AtRAD51 and AtDMC1.• The results demonstrate that AtBRCA2 is important for both somatic and meiotic homologous recombination. We further show that AtBRCA2 is required for proper meiotic synapsis and mediates the recruitment of AtRAD51 and AtDMC1. Our results suggest that BRCA2 controls single-strand invasion steps during homologous recombination in plants.

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“…Hays used a root growth assay to show that polymerase ζ and polymerase η single mutants show cell death on UVB irradiation and that double mutants suffered similar cell death even in the absence of UVB treatment. Mutants in BRCA1 (breast cancer 1) and BARD1 (BRCA1 associated RING domain 1) homologues were shown by H. Puchta (Karlsruhe, Germany) to be sensitive to the DNA crosslinking agent mitomycin C and to be deficient in somatic homologous recombination, suggesting a functional conservation between mammals and plants (Reidt et al, 2006). Furthermore, the RECQ4A gene of plants might be a functional equivalent of the Bloom syndrome counterpart in humans, based on the hyperrecombination phenotype of its knockout mutant and its genetic interaction with the gene for endonuclease MUS81.…”

Section: Somatic Dna Repair and Recombinationmentioning

confidence: 99%

Plant DNA repair and recombination on the French Mediterranean

Copenhaver

Hooykaas

2007

EMBO Reports

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A homologue of the breast cancer-associated gene BARD1 is involved in DNA repair in plants

Cited by 4 publications

References 28 publications

Arabidopsis RETINOBLASTOMA RELATED directly regulates DNA damage responses through functions beyond cell cycle control

Arabidopsis RETINOBLASTOMA RELATED directly regulates DNA damage responses through functions beyond cell cycle control

BRCA2 is a mediator of RAD51‐ and DMC1‐facilitated homologous recombination inArabidopsis thaliana

Plant DNA repair and recombination on the French Mediterranean

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