A Homeodomain-Containing Transcriptional Factor PoHtf1 Regulated the Development and Cellulase Expression in Penicillium oxalicum

Guo, Hao; Xu, Gen; Wu, Ruimei; Li, Zhigang; Yan, Mengdi; Jia, Zhilei; Li, Zhonghai; Chen, Mei; Bao, Xinhe; Qu, Yinbo

doi:10.3389/fmicb.2021.671089

Cited by 7 publications

(3 citation statements)

References 34 publications

(57 reference statements)

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“…P. oxalicum is also used as a model strain for study of the regulatory mechanisms of cellulase synthesis and asexual development; this research benefits from its mature genetic engineering system. The efficient synthesis of cellulase and several developmental processes (e.g., mycelial growth and conidiation) are co-regulated synergistically by multiple transcription factors in P. oxalicum (Li et al, 2015(Li et al, , 2020Guo et al, 2021;Xu et al, 2022). The regulation of these processes in filamentous fungi is usually triggered by environmental signals (i.e., soluble carbon sources and light), which act on target genes through an intracellular signaling pathway, i.e., the G protein-cAMP signaling pathway (Hu et al, 2013;Cabrera et al, 2022;Schmoll and Hinterdobler, 2022).…”

Section: Introductionmentioning

confidence: 99%

Phosducin-like protein PoPlp1 impacts cellulase and amylase expression and development in Penicillium oxalicum via the G protein–cAMP signaling pathway

Jia,

Yan,

et al. 2023

Front. Microbiol.

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In this study, a phosducin-like protein, PoPlp1, was identified and functionally studied in the cellulase-producing strain Penicillium oxalicum 114-2. PoPlp1 was proven to participate in several biological processes, including mycelium development, conidiation, and expression of cellulases and amylases. With deletion of Poplp1, morphology and development varied significantly in ΔPoplp1. Colony growth, glucose utilization, and the hydrolysis capability of starch and cellulose were limited, whereas conidiation was enhanced. Based on detection of the levels of expression of transcription factors involved in asexual development, we conjectured that PoPlp1 is involved in conidiation via the major factor BrlA. We explored the effect of PoPlp1 on cellulase and amylase expression and observed that cellulase and amylase activity and major gene transcription levels were all dramatically reduced in ΔPoplp1. Deletion of PoPlp1 caused a decrease in intracellular cAMP levels, and the cellulase gene expression level of ΔPoplp1 was restored to a certain extent through external addition of cAMP. These findings demonstrate that PoPlp1 may affect cellulase and amylase expression by regulating cAMP concentration. To comprehensively explore the mechanism of PoPlp1 in regulating multiple biological processes, we performed a comparative transcriptomic analysis between strains P. oxalicum 114-2 and ΔPoplp1. The major cellulase and amylase genes were all downregulated, congrent with the results of real-time quantitative polymerase chain reaction analysis. The genes involved in the G protein–cAMP signaling pathway, including several G-protein-coupled receptors, one regulator of G protein signaling, and two cAMP phosphodiesterases, were disrupted by deletion of PoPlp1. These results confirm the positive function of PoPlp1 in the G protein–cAMP signaling pathway. This functional analysis of PoPlp1 will be very beneficial for further study of the regulatory mechanisms of cellulase expression and other biological processes in P. oxalicum 114-2 via the G protein–cAMP signaling pathway.

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Section: Introductionmentioning

confidence: 99%

Phosducin-like protein PoPlp1 impacts cellulase and amylase expression and development in Penicillium oxalicum via the G protein–cAMP signaling pathway

Jia,

Yan,

et al. 2023

Front. Microbiol.

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“…In addition to the direct transcription factors, some regulators in other pathways are also found to involve in the cellulase regulation network. For example, the transcription factors regulating development, BrlA (Qin et al, 2013), FlbC (Yao et al, 2016) and Po Htf1 (Guo et al, 2021), and transcription factor controlling amino acid biosynthesis, CpcA (Pan et al, 2020), also play important biological roles in the cellulase production in P. oxalicum .…”

Section: Introductionmentioning

confidence: 99%

An actin-like protein PoARP9 involves in the regulation of development and cellulase and amylase expression in Penicillium oxalicum

Guo

Yan

et al. 2022

Journal of Applied Microbiology

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Aims:In eukaryotic cells, chromatin remodelling complexes are essential for the accessibility of transcription factors to the specific regulating regions of downstream genes. Here, we identified an actin-like protein PoARP9 in cellulase production strain Penicillium oxalicum 114-2, which was an essential member of SWI/SNF complex. To investigate the physiological function of PoARP9 in transcriptional regulation, the coding gene Poarp9 was deleted in P. oxalicum 114-2. Methods and Results:The absence of PoARP9 affected the colony growth on medium with glucose, cellulose or starch as sole carbon source. Meanwhile, the expression levels of major cellulase genes were all upregulated in ΔPoarp9 under the cellulase-inducing condition. In addition, the expression levels of amylase transcription activator AmyR as well as two major amylase genes were also increased in ΔPoarp9. Conclusions:These results demonstrated that chromatin remodelling affects the development and expression of cellulase and amylase in P. oxalicum. And the SWI/ SNF complex member PoARP9 plays essential roles in these processes. Significance and Impact of the Study:This study provided new insights into the regulation of cellulase and development in P. oxalicum. And the regulatory function of SWI/SNF complex member ARP9 towards cellulase and amylase expression in P. oxalicum was verified for the first time.

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“…CBH is considered a key component of the cellulase system, and its main product acting on cellulose is cellobiose [ 21 ]. The CEL6B gene encoding CBH has been identified in L. edodes [ 22 , 23 ].…”

Section: Introductionmentioning

confidence: 99%

Transcription factor FfMYB15 regulates the expression of cellulase gene FfCEL6B during mycelial growth of Flammulina filiformis

et al. 2022

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Background Cellulose degradation can determine mycelial growth rate and affect yield during the growth of Flammulina filiformis. The degradation of cellulose requires the joint action of a variety of cellulases, and some cellulase-related genes have been detected in mushrooms. However, little is known about the transcriptional regulatory mechanisms of cellulose degradation. Results In this study, FfMYB15 that may regulate the expression of cellulase gene FfCEL6B in F. filiformis was identified. RNA interference (RNAi) showed that FfCEL6B positively regulated mycelial growth. Gene expression analyses indicated that the expression patterns of FfCEL6B and FfMYB15 in mycelia cultured on the 0.9% cellulose medium for different times were similar with a correlation coefficient of 0.953. Subcellular localization and transcriptional activity analyses implied that FfMYB15 was located in the nucleus and was a transcriptional activator. Electrophoretic mobility shift assay (EMSA) and dual-luciferase assays demonstrated that FfMYB15 could bind and activate FfCEL6B promoter by recognizing MYB cis-acting element. Conclusions This study indicated that FfCEL6B played an active role in mycelial growth of F. filiformis and was regulated by FfMYB15.

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A Homeodomain-Containing Transcriptional Factor PoHtf1 Regulated the Development and Cellulase Expression in Penicillium oxalicum

Cited by 7 publications

References 34 publications

Phosducin-like protein PoPlp1 impacts cellulase and amylase expression and development in Penicillium oxalicum via the G protein–cAMP signaling pathway

Phosducin-like protein PoPlp1 impacts cellulase and amylase expression and development in Penicillium oxalicum via the G protein–cAMP signaling pathway

An actin-like protein PoARP9 involves in the regulation of development and cellulase and amylase expression in Penicillium oxalicum

Transcription factor FfMYB15 regulates the expression of cellulase gene FfCEL6B during mycelial growth of Flammulina filiformis

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