Abstract:We examined the possible effects of elemental mercury vapor on the liver of the female rats. We divided the animals into an untreated control group and an experimental group that was exposed to mercury vapor for 45 days. Liver samples were obtained for histological and stereological analysis. The total liver, parenchyma and sinusoid volumes were increased significantly in the mercury vapor treated group compared to controls. Also, the mean density, total number and mean nuclear diameter of hepatocytes, except … Show more
“…Subsequently, samples were embedded in Araldite CY 212. [ 19 20 ] Semi-thin (1 μm thick) and ultra-thin sections (80 nm thick) were cut using a Leica RM2125RT microtome (Gaintenbain Comp., Ankara, Turkey) and ultramicrotome (Nova LKB, Bromma, Sweden), respectively. Semi-thin sections were stained with toluidine blue for the stereological and histological examination in the light microscope.…”
Background:
Umbilical cord plays a crucial role in the continuation of pregnancy by transferring nutrition and oxygen across the placenta to the fetus. We aimed to investigate the morphometrical and histological features of the umbilical cords in new-born rats.
Materials and Methods:
The adult male and female rats were chosen for matting purpose in the present study. Briefly, ten adult
Wistar albino
rats (female,
n
= 5; male,
n
= 5) were randomly assigned into five groups of two animals (female,
n
= 1; male,
n
= 1). Immediately after parturition, two umbilical cords of new-born rats (0-day-old) from each group were randomly selected. Finally, ten umbilical cord samples were examined using the histological and stereological methods in the light and electron microscopes.
Results:
The total numbers of Hofbauer cells and mesenchymal stromal cells was estimated statistically. We also calculated the mean volume of umbilical cords, arteries and veins, as well as arterial and venous lumens. Our histological findings also exhibited the histological features of Hofbauer cells, mesenchymal stromal cell cells, and blood vessels.
Conclusion:
Our findings showed more detailed information about umbilical cord tissues and their components, and that may contribute to the diagnose of umbilical cord complications in the developing fetus.
“…Subsequently, samples were embedded in Araldite CY 212. [ 19 20 ] Semi-thin (1 μm thick) and ultra-thin sections (80 nm thick) were cut using a Leica RM2125RT microtome (Gaintenbain Comp., Ankara, Turkey) and ultramicrotome (Nova LKB, Bromma, Sweden), respectively. Semi-thin sections were stained with toluidine blue for the stereological and histological examination in the light microscope.…”
Background:
Umbilical cord plays a crucial role in the continuation of pregnancy by transferring nutrition and oxygen across the placenta to the fetus. We aimed to investigate the morphometrical and histological features of the umbilical cords in new-born rats.
Materials and Methods:
The adult male and female rats were chosen for matting purpose in the present study. Briefly, ten adult
Wistar albino
rats (female,
n
= 5; male,
n
= 5) were randomly assigned into five groups of two animals (female,
n
= 1; male,
n
= 1). Immediately after parturition, two umbilical cords of new-born rats (0-day-old) from each group were randomly selected. Finally, ten umbilical cord samples were examined using the histological and stereological methods in the light and electron microscopes.
Results:
The total numbers of Hofbauer cells and mesenchymal stromal cells was estimated statistically. We also calculated the mean volume of umbilical cords, arteries and veins, as well as arterial and venous lumens. Our histological findings also exhibited the histological features of Hofbauer cells, mesenchymal stromal cell cells, and blood vessels.
Conclusion:
Our findings showed more detailed information about umbilical cord tissues and their components, and that may contribute to the diagnose of umbilical cord complications in the developing fetus.
“…The mean volume of sinusoids and livers was calculated using both the Cavalieri and the point-counting methods (25,26). The systematic randomly sampling technique was performed to obtain the accurate and valuable results.…”
OBJECTIVE: We aimed to investigate the possible effect of topiramate (TOP, 0.02 mg/kg/day) on the livers in a high-fat diet (HFD)-induced obesity rat model. The other objective was to evaluate the relationship between TOP administration and NPY level using anti-NPY1R antibody. METHODS: Twenty-four adult female Wistar albino rats were randomly assigned into four equal groups as follow: control (CONT), obese (OBS), TOP, and OBS+TOP. All liver samples were investigated using the stereological analysis, as well as immunohistochemical and histopathological examination. RESULTS: The total number of hepatocytes was signifi cantly decreased in the OBS+TOP group compared to the CONT group or the OBS group (p < 0.05). We found a signifi cant increase in the mean volume of liver in the OBS group compared to the CONT group (p < 0.05). Also, the mean volume of liver was signifi cantly decreased in the OBS+TOP group compared to the OBS group (p < 0.05). CONCLUSION: Taken together, our fi ndings suggest that decreased liver volume is possibly attributed to TOP administration via setting the NPY level in the obese rats. Further, the side effects of TOP in combination with health risk of obesity may have led to an increase in hepatotoxicity and the subsequent hepatocyte loss (Fig. 7, Ref. 56).
“…The mercury concentration of the chamber was measured every hour using a Jerome Model 431-X mercury analyzer (Arizona Instruments, Phoenix AR). After 17:00 h, the mercury vapor unit attached to the chamber was turned off and the animals remained in the closed chamber without exposure to mercury vapor during the night (Altunkaynak et al 2016, Yahyazedeh et al 2017.…”
Mercury in the environment that arises from organic and inorganic sources can cause irreversible damage to the nervous system. Toxicity may be direct or may arise from interactions with other metals in the environment. We evaluated the possible effects of mercury vapor on rat cerebellum. Twelve adult female rats were divided into control and experimental groups. The rats in the experimental group were exposed to mercury vapor for 9 h/day for 45 days. Cerebellar tissue samples were evaluated using stereology and for histopathology. The total number of Purkinje cells was estimated using a physical disector method. We found that in the experimental group, overall volume decreased and the number of Purkinje cells was reduced. We also found cellular damage including pycnotic nuclei, eosinophilic cytoplasm and vacuolization; these features were absent in the control group. We found that chronic exposure to inorganic mercury vapor is toxic to the cerebellum.
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