A histone H2A derived antimicrobial peptide, Fi-Histin from the Indian White shrimp, Fenneropenaeus indicus: Molecular and functional characterization

Sruthy, K S; Nair, Aishwarya; Antony, Swapna P.; Puthumana, Jayesh; Singh, I. S. Bright; Philip, Rosamma

doi:10.1016/j.fsi.2019.06.044

Cited by 29 publications

(18 citation statements)

References 48 publications

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“…Histones H2A, H2B, H3, and H4 were identified to be deiminated in the Mollusca in the current study and these are known deimination targets with roles in epigenetic regulation and antipathogenic responses in a range of taxa [23][24][25][26]47] as well as in relation to gene regulation in human pathologies, including cancer [37,78,79]. Histones serve as antimicrobial compounds in various species, ranging from crustaceans [80,81], amphibian [82], teleost fish [21,83], reptiles [84], pinnipeds and cetaceans [49,85], to human [86], including in mucosal immunity [87]. Histones have also been identified to have antimicrobial properties in Mollusca, for example H2A derived ones in disk abalone [88] and scallop [89], and histones H2B and H4 in oyster [90,91], where extracellular release of antimicrobial histones (extracellular trap formation/ETosis) is triggered by ROS [92,93].…”

Section: Discussionmentioning

confidence: 64%

Extracellular Vesicles and Post-Translational Protein Deimination Signatures in Mollusca—The Blue Mussel (Mytilus edulis), Soft Shell Clam (Mya arenaria), Eastern Oyster (Crassostrea virginica) and Atlantic Jacknife Clam (Ensis leei)

Bowden

Kraev

Lange

2020

Biology

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Oysters and clams are important for food security and of commercial value worldwide. They are affected by anthropogenic changes and opportunistic pathogens and can be indicators of changes in ocean environments. Therefore, studies into biomarker discovery are of considerable value. This study aimed at assessing extracellular vesicle (EV) signatures and post-translational protein deimination profiles of hemolymph from four commercially valuable Mollusca species, the blue mussel (Mytilus edulis), soft shell clam (Mya arenaria), Eastern oyster (Crassostrea virginica), and Atlantic jacknife clam (Ensis leei). EVs form part of cellular communication by transporting protein and genetic cargo and play roles in immunity and host–pathogen interactions. Protein deimination is a post-translational modification caused by peptidylarginine deiminases (PADs), and can facilitate protein moonlighting in health and disease. The current study identified hemolymph-EV profiles in the four Mollusca species, revealing some species differences. Deiminated protein candidates differed in hemolymph between the species, with some common targets between all four species (e.g., histone H3 and H4, actin, and GAPDH), while other hits were species-specific; in blue mussel these included heavy metal binding protein, heat shock proteins 60 and 90, 2-phospho-D-glycerate hydrolyase, GTP cyclohydrolase feedback regulatory protein, sodium/potassium-transporting ATPase, and fibrinogen domain containing protein. In soft shell clam specific deimination hits included dynein, MCM3-associated protein, and SCRN. In Eastern oyster specific deimination hits included muscle LIM protein, beta-1,3-glucan-binding protein, myosin heavy chain, thaumatin-like protein, vWFA domain-containing protein, BTB domain-containing protein, amylase, and beta-catenin. Deiminated proteins specific to Atlantic jackknife clam included nacre c1q domain-containing protein and PDZ domain-containing protein In addition, some proteins were common as deiminated targets between two or three of the Bivalvia species under study (e.g., EP protein, C1q domain containing protein, histone H2B, tubulin, elongation factor 1-alpha, dominin, extracellular superoxide dismutase). Protein interaction network analysis for the deiminated protein hits revealed major pathways relevant for immunity and metabolism, providing novel insights into post-translational regulation via deimination. The study contributes to EV characterization in diverse taxa and understanding of roles for PAD-mediated regulation of immune and metabolic pathways throughout phylogeny.

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Section: Discussionmentioning

confidence: 64%

Extracellular Vesicles and Post-Translational Protein Deimination Signatures in Mollusca—The Blue Mussel (Mytilus edulis), Soft Shell Clam (Mya arenaria), Eastern Oyster (Crassostrea virginica) and Atlantic Jacknife Clam (Ensis leei)

Bowden

Kraev

Lange

2020

Biology

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“…Histone H3 deimination is in many species associated with the trapping of foreign pathogens via extracellular traps, as reported in a range of taxa [ 85 ]. Histones can furthermore serve as antimicrobial compounds as reported in humans [ 86 ] and various other taxa including molluscs [ 87 , 88 , 89 , 90 ], crustaceans [ 91 , 92 ], amphibians [ 93 ], teleosts [ 94 ], reptiles [ 95 ], and pinnipeds [ 96 ]. However, histone H3 deimination has also been shown to relate to the loss of bactericidal activity [ 97 ].…”

Section: Discussionmentioning

confidence: 99%

Extracellular Vesicle Signatures and Post-Translational Protein Deimination in Purple Sea Urchin (Strongylocentrotus purpuratus) Coelomic Fluid—Novel Insights into Echinodermata Biology

D’Alessio

Buckley

Kraev

et al. 2021

Biology

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The purple sea urchin (Strongylocentrotus purpuratus) is a marine invertebrate of the class Echinoidea that serves as an important research model for developmental biology, cell biology, and immunology, as well as for understanding regenerative responses and ageing. Peptidylarginine deiminases (PADs) are calcium-dependent enzymes that mediate post-translational protein deimination/citrullination. These alterations affect protein function and may also play roles in protein moonlighting. Extracellular vesicles (EVs) are membrane-bound vesicles that are released from cells as a means of cellular communication. Their cargo includes a range of protein and RNA molecules. EVs can be isolated from many body fluids and are therefore used as biomarkers in physiological and pathological responses. This study assessed EVs present in the coelomic fluid of the purple sea urchin (Strongylocentrotus purpuratus), and identified both total protein cargo as well as the deiminated protein cargo. Deiminated proteins in coelomic fluid EVs were compared with the total deiminated proteins identified in coelomic fluid to assess putative differences in deiminated protein targets. Functional protein network analysis for deiminated proteins revealed pathways for immune, metabolic, and gene regulatory functions within both total coelomic fluid and EVs. Key KEGG and GO pathways for total EV protein cargo furthermore showed some overlap with deimination-enriched pathways. The findings presented in this study add to current understanding of how post-translational deimination may shape immunity across the phylogeny tree, including possibly via PAD activity from microbiota symbionts. Furthermore, this study provides a platform for research on EVs as biomarkers in sea urchin models.

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“…Antimicrobial peptides (AMPs) are produced as first line of defense by all eukaryotic organisms with broad activity against microbes and cancer cells (Izadpanah and Gallo 2005). AMPs produced from precursor proteins by proteolytic cleavage are cationic with significant amount of hydrophobic residues (Boman 2003;Sruthy et al 2019). Histones play an important role in the structural organisation of chromatin and also in post-translational modifications, crucial for gene regulation (Parseghian and Luhrs 2006;Poirier et al 2014).…”

Section: Introductionmentioning

confidence: 99%

“…indicus (Sruthy et al 2019). H2A-derived AMPs have been reported from fishes (Vertebrates) viz., Parasilurus asotus (Park et al 1998), Salmo salar (Richards et al 2001), Hippoglossus hippoglossus (Birkemo et al 2003), Oncorhynchus mykiss (Fernandes et al 2002), Tachysurus jella, Cynoglossus semifasciatus (Chaithanya et al 2013) and Danio rerio (Caccia et al 2017).…”

Section: Introductionmentioning

confidence: 99%

A histone H2A-derived antimicrobial peptide, Hipposin from mangrove whip ray, Himantura walga: Molecular and functional characterisation

et al. 2020

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Antimicrobial peptides (AMPs) are biologically dynamic molecules produced by all type of organisms as a fundamental component of their innate immune system. The present study deals with the identification of a histone H2A-derived antimicrobial peptide, Hipposin from mangrove whip ray, Himantura walga. A 243 base pair fragment encoding 81 amino acid residues amplified from complementary DNA was identified as Hipposin and termed as Hw-Hip. Homologous analysis showed that Hw-Hip belongs to the Histone H2A superfamily and shares sequence identity with other histone-derived AMPs from fishes. Phylogenetic analysis of Hw-Hip displayed clustering with the fish H2A histones. Secondary structure analysis showed the presence of three α-helices and four random coils with a prominent proline hinge. The physicochemical properties of Hw-Hip are in agreement with the properties of antimicrobial peptides. A 39-mer active peptide sequence was released by proteolytic cleavage in silico. Functional characterisation of active peptide in silico revealed antibacterial, anticancer and antibiofilm activities making Hw-Hip a promising candidate for further exploration.

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A histone H2A derived antimicrobial peptide, Fi-Histin from the Indian White shrimp, Fenneropenaeus indicus: Molecular and functional characterization

Cited by 29 publications

References 48 publications

Extracellular Vesicles and Post-Translational Protein Deimination Signatures in Mollusca—The Blue Mussel (Mytilus edulis), Soft Shell Clam (Mya arenaria), Eastern Oyster (Crassostrea virginica) and Atlantic Jacknife Clam (Ensis leei)

Extracellular Vesicles and Post-Translational Protein Deimination Signatures in Mollusca—The Blue Mussel (Mytilus edulis), Soft Shell Clam (Mya arenaria), Eastern Oyster (Crassostrea virginica) and Atlantic Jacknife Clam (Ensis leei)

Extracellular Vesicle Signatures and Post-Translational Protein Deimination in Purple Sea Urchin (Strongylocentrotus purpuratus) Coelomic Fluid—Novel Insights into Echinodermata Biology

A histone H2A-derived antimicrobial peptide, Hipposin from mangrove whip ray, Himantura walga: Molecular and functional characterisation

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