A histomorphometric study of the effect of doxycycline and erythromycin on bone formation in dental alveolar socket of rat

Shahabooei, Mohammad; Razavi, Sayed Mohammad; Minaiyan, Mohsen; Birang, Reza; Behfarnia, Parichehr; Yaghini, Jaber; Naghsh, Narges; Ghalayani, Parichehr; Hajisadeghi, Samira

doi:10.4103/2277-9175.153895

Cited by 17 publications

(11 citation statements)

References 29 publications

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“… 28 Recently, low doses of orally administered DOX for 7 days have been found to enhance bone formation in the alveolar sockets of rats. 29 The bone matrix is composed primarily of type I collagen fibres, which are a marker of osteoblast differentiation. Bone strength depends on more than quantity and mainly depends on quality, which is characterised by the geometry and shape of bones, the microarchitecture of trabecular bone, bone turnover, and the presence of minerals and collagen.…”

Section: Discussionmentioning

confidence: 99%

Doxycycline induces bone repair and changes in Wnt signalling

et al. 2017

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Doxycycline (DOX) exhibits anti-inflammatory and MMP inhibitory properties. The objectives of this study were to evaluate the effects of DOX on alveolar bone repair. Controls (CTL) and DOX-treated (10 and 25 mg·kg−1) molars were extracted, and rats were killed 7 or 14 days later. The maxillae were processed and subjected to histological and immunohistochemical assays. Hematoxylin-eosin staining (7th day) revealed inflammation in the CTL group that was partly reversed after DOX treatment. On the 14th day, the CTL group exhibited bone neoformation, conjunctive tissue, re-epithelization and the absence of inflammatory infiltrate. DOX-treated groups exhibited complete re-epithelization, tissue remodelling and almost no inflammation. Picrosirius red staining in the DOX10 group (7th and 14th days) revealed an increased percentage of type I and III collagen fibres compared with the CTL and DOX25 groups. The DOX10 and DOX25 groups exhibited increases in osteoblasts on the 7th and 14th days. However, there were fewer osteoclasts in the DOX10 and DOX25 groups on the 7th and 14th days. Wnt-10b-immunopositive cells increased by 130% and 150% on the 7th and 14th days, respectively, in DOX-treated groups compared with the CTL group. On the 7th day, Dickkopf (Dkk)-1 immunostaining was decreased by 63% and 46% in the DOX10 and DOX25 groups, respectively. On the 14th day, 69% and 42% decreases in immunopositive cells were observed in the DOX10 and DOX25 groups, respectively, compared with the CTL group. By increasing osteoblasts, decreasing osteoclasts, activating Wnt 10b and neutralising Dkk, DOX is a potential candidate for bone repair in periodontal diseases.

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Section: Discussionmentioning

confidence: 99%

Doxycycline induces bone repair and changes in Wnt signalling

et al. 2017

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“…Another study performed in dogs, showed that doxycycline is able to reduce bone loss caused by endodontic peri radicular surgery 77 . However, another study showed that doxycycline does not improved the quantity and quality of tissue repair on bone in Wistar rats subjected to dental extraction and subsequently treated for 7 days on doxycycline and erythromycin 78 . This fact may be comprehended in this previous cited work, explaining that the rats were subjected to a short period of treatment with doxycycline (7 days) and the quantification was injured because of this short time of treatment.…”

Section: Discussionmentioning

confidence: 98%

Doxycycline reduces osteopenia in female rats

Figueiredo

Shimano

Ervolino

et al. 2019

Sci Rep

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Doxycycline, a member of the tetracycline family, is a drug used as an antibiotic (dosage of 100 mg/day) and as an anti-inflammatory drug on the dosage of 20 mg twice a day, this use has Matrix Metalloproteinases (MMP) inhibitor action. Doxycycline is a calcium chelator and therefore interferes in bone remodeling. The main objective of this study was to evaluate the action of the drug doxycycline in the control of osteopenia. Sixty three Wistars rats were divided into 9 groups with n = 7 each, as follow: the control group with doxycycline 10 mg/kg/day (C10), control with doxycycline 30 mg/kg/day (C30) and control (C), ovariectomized group with doxycycline 10 mg/kg/day (OVX10), ovariectomized with doxycycline 30 mg/kg/day (OVX30), and ovariectomized with water (OVX), sedentary group with 10 mg/kg/day (Se10), sedentary with doxycycline 30 mg/kg/day (Se30), and sedentary group with water (Se). Left femoral bone was used for bone densitometry, right femoral bone for histological analysis. The right tibia was intended for chemical quantifications, the total serum was used for cholesterol and calcium quantification. The length of the left femoral bone was measured after the densitometry analysis. Statistical analysis was performed using multivariate general linear model (ANOVA two factors with Bonferroni adjustment) and the TRAP analysis was subjected to normality test and then were subjected to nonparametric test, both with p < 0.05 significance. Statistically significant differences were found, with better results for the groups exposed to the medication (10 and 30 mg/kg/day): Se vs. Se10 and Se vs. Se30 for BMC, quantification of magnesium, amount of cancellous bone in the distal portion; OVX vs. OVX10 for BMC, BMD and calcium in serum; OVX vs. OVX10 and OVX30 for quantification in proximal and distal portion of cancellous bone; Se vs. Se30 and OVX vs. OVX30 for immunostaining for TRAP, all results with minimum of p ≤ 0.05. Doxycycline had a deleterious effect on control groups and positive action for bone organization on female rats affected by bilateral ovariectomy-induced osteopenia and sedentary lifestyle.

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“…Erythromycin has bacteriostatic activities and is commonly prescribed for dental caries and dental plaque [ 55 , 56 , 58 ]. The most common microorganism that causes dental caries is Streptococcus mutans , which is highly sensitive to erythromycin [ 59 ].…”

Section: The Most Common Prescribed Antibioticsmentioning

confidence: 99%

Antibiotic Therapy in Dentistry

Ahmadi

Ebrahimi

Ahmadi

2021

International Journal of Dentistry

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Dental caries, pulpal necrosis, trauma, and periodontal diseases can result in dental infections which could have severe consequences that affect both soft and hard tissues of the oral cavity. Dental infections commonly present with symptoms of pain, fever, and swelling. Surgical and endodontic treatments are the early management of infected teeth, followed by antibiotic therapy. Some alternative methods also exist for treating infection such as low-level laser therapy and photodynamic therapy. Antibiotics are generally used in dental procedures to treat odontogenic infections, nonodontogenic infections, local infection, focal infection, and prophylaxis. Antibiotic prophylaxis is prescribed for patients with immunosuppressed conditions, infective endocarditis, metabolic disorders, and patients with prosthetic joints. To reduce the complications of unnecessary antibiotic prescriptions especially bacterial resistance, comprehensive guidelines should be established. It has been noted that only about 12% of dentists adequately and correctly prescribe antibiotics, which shows the importance of comprehensive guidelines. Antibiotics prescription may result in some adverse effects such as hypersensitivity reactions and dermatological and allergic disorders. Furthermore, unnecessary prescription of antibiotics could result in several serious sequelae, for example, bacterial resistance, gastric and hematological problems, and diversion of bacterial microbiota. The present review attempts to summarize the indications of antibiotic therapy in dentistry and discuss the common types of antibiotics that are routinely used in dental practice based on pharmacologic classes. Moreover, types of antibiotics that are considered safe during pregnancy and childhood are also reviewed.

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A histomorphometric study of the effect of doxycycline and erythromycin on bone formation in dental alveolar socket of rat

Cited by 17 publications

References 29 publications

Doxycycline induces bone repair and changes in Wnt signalling

Doxycycline induces bone repair and changes in Wnt signalling

Doxycycline reduces osteopenia in female rats

Antibiotic Therapy in Dentistry

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