A histomorphometric classification system for normal and inflamed mouse duodenum—Quali‐quantitative approach

Silva, Airton Pereira e; Soares, João Ricardo Almeida; Mattos, Érika Bertozzi de Aquino; Josetti, Claudia; Guimarães, Isabelle Mazza; Campos, Sylvia Maria Nicolau; Teixeira, Gerlinde Agate Platais Brasil

doi:10.1111/iep.12286

Cited by 5 publications

(8 citation statements)

References 54 publications

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“…The decrease in villus width in the high-iron group may be related to the decreased crypt depth at 3 DPA. According to the study by Holle, GE ( 38 ), the proliferative zone in the crypt expanded in proportion to the total crypt depth. However, the results of studies examining the effects of a high iron concentration on the morphology of the small intestine are contradictory.…”

Section: Discussionmentioning

confidence: 99%

Dietary High Dose of Iron Aggravates the Intestinal Injury but Promotes Intestinal Regeneration by Regulating Intestinal Stem Cells Activity in Adult Mice With Dextran Sodium Sulfate-Induced Colitis

et al. 2022

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The effects of excessive dietary iron intake on the body have been an important topic. The purpose of this study was to investigate the effects of high-dose iron on intestinal damage and regeneration in dextran sodium sulfate (DSS)-induced colitis model mice. A total of 72 8-week-old adult C57BL/6 mice were randomly divided into two dietary treatment groups: the basal diet supplemented with 45 (control) and 450 mg/kg iron (high-iron) from ferrous sulfate. The mice were fed different diets for 2 weeks, and then 2.5% DSS was orally administered to all mice for 7 days. Samples of different tissues were collected on days 0, 3, and 7 post administration (DPA). High-iron treatment significantly decreased the relative weight of the large intestine at 7 DPA but not at 0 DPA or 3 DPA. High dietary iron increased the jejunal villus width at 0 DPA, decreased the villus width and the crypt depth of the jejunum at 3 DPA, and decreased the number of colonic crypts at 7 DPA. Meanwhile, high dietary iron decreased the number of goblet cells in the jejunal villi and the Paneth cells in the jejunal crypts at 0 DPA, increased the number of goblet cells per crypt of the colon at 3 DPA, and the number of Paneth cells in the jejunal crypts, the goblet cells in the colon, the Ki67-positive proliferating cells in the colon, and the Sex-determining region Y-box transcription factor 9+ (SOX9) cells in the jejunum crypts and colon at 7 DPA. The organoid formation rate was increased by high-iron treatments at 3 DPA and 7 DPA. High dietary iron treatment decreased the mRNA level of jejunal jagged canonical Notch ligand 2 (Jag-2) at 0 DPA and bone morphogenetic protein 4 (Bmp4) and neural precursor cell-expressed developmentally downregulated 8 (Nedd8) in the jejunum and colon at 7 DPA, whereas it increased the mRNA expression of the serum/glucocorticoid-regulated kinase 1 (Sgk1) in the colon at 3 DPA. The results suggested that a high dose of iron aggravated intestinal injury but promoted intestinal repair by regulating intestinal epithelial cell renewal and intestinal stem cell activity in adult mice with colitis.

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Section: Discussionmentioning

confidence: 99%

Dietary High Dose of Iron Aggravates the Intestinal Injury but Promotes Intestinal Regeneration by Regulating Intestinal Stem Cells Activity in Adult Mice With Dextran Sodium Sulfate-Induced Colitis

et al. 2022

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“…Villus area is a parameter still poorly described in the literature. Studies from our group have already proposed that this is an important aspect to be assessed since the identification of the occurrence of histological adaptation may indicate a change in the absorption surface of the small intestine 31,32,68 .…”

Section: Discussionmentioning

confidence: 99%

Prior Food-Induced Allergic Gut Inflammation Leading To Multiple Food Allergies in C57Bl/6 Mice

Silva¹,

Marmello²,

et al. 2020

Preprint

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BACKGROUND: Food allergies are usually managed by food avoidance. Hidden allergens in food, due to cross-contamination and/or allergenic additives added during production, place an important concern in today's increasing food allergy cases worldwide. Previous studies showed that introduction of new food components, in an inflamed intestine, results in sensitization to this food. Thus, our aim was to evaluate the kinetics of multiple food allergy induction. METHODS: Adult male C57BL/6 mice were divided into five groups, four of which were submitted to an intestinal inflammation induction protocol to peanuts. Egg white (OVA) diluted 1:5 v/v in distilled water was instilled by gavage 6h-before (EXP-1), concomitant (EXP-2) and 6h-after (EXP-3) the onset of the peanut challenge diet. Positive control (POS CONT) and NEG CONT received saline per gavage. Finally, animals were challenged with subcutaneous injections of OVA. RESULTS: No changes in diet intake were observed. Anti-OVA total IgG antibody titers significantly increased in EXP-2. Flow cytometry revealed significant decrease in CD4+CD25+Foxp3+ and significant increase in TCD8+ in EXP-2. Histomorphometrically, EXP-2 and EXP-3 were classified as Infiltrative and Partial Destruction stages. EXP-1 was classified as Infiltrative, while POS CONT was classified as Partial Destruction. NEG CONT was classified as Normal. CONCLUSION: The introduction of a new food only a few hours before the initiation of a gut inflammation is able to induce oral tolerance, however the introduction of a new dietary protein concomitant to the onset or during an ongoing gut inflammation may induce multiple allergies.

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“…were classified as Normal in our system and EXP-2 (Figure 5C)was staged as Partial Destruction, which is the third level of a 5-level classification system [4]. 8A).…”

Section: Histopathological Evaluation Of Intestinal Samplesmentioning

confidence: 99%

“…Tissue sections were scanned using Aperio ® Scanscope (Leica Microsystems GmbH, Wetzlar, Germany) and then analyzed with ImageScope software (version 11.2.0.780; Leica Microsystems GmbH, Wetzlar, Germany) to quantify histological parameters. A histomorphometric classification system for normal and inflamed mouse duodenum developed by our group [35] was used to better stage the intestinal situation of each animal.…”

Section: Determination Of T and B Lymphocyte Profilementioning

confidence: 99%

Expansion of Antigen- Specific Memory Cells as a Potential Booster for Food Tolerance Induction

Silva¹,

Marmello²,

Bentes³

et al. 2023

Preprint

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Approximately 3% of children in Western countries are diagnosed with peanut allergy, a likely lifelong disease. The preferred treatment for food allergy is allergen avoidance. However, oral immunotherapy is an FDA-approved treatment to re-induce tolerance, still, not all patients respond as expected. Thus, the aim of this work is to evaluate whether the association of an antigen-specific tolerogenic (oral tolerance) bystander effect can ameliorate the recovery of inflamed intestinal mucosa. Adult male C57BL/6 mice were divided into five groups, four of which were submitted to an intestinal inflammation induction protocol to peanuts. After sensitization, experimental groups were orally challenged with either peanuts or a hybrid diet (peanuts + mouse chow). In a second stage, groups were sensitized, challenged with peanuts, and then received either peanuts, hybrid diet, or ovalbumin chow during the recovery period of the inflamed mucosa. Results showed no changes in diet intake and body weight. Polyisotypic anti-peanut IgG and IgG1 were significantly increased in the serum from animals in allergic groups. The group that received the hybrid diet showed an increase in CD4+CD25+Foxp3+ regulatory T cells, as well as in B220+CD3-CD27+ memory B cells. Histology of the duodenum showed a decrease in intraepithelial leukocytes in animals who received hybrid diet. Together, our results show that when the tolerogen is added to a diet containing the allergen, it can ameliorate the induction of local inflammation. Simultaneously offering the allergen with a tolerated food increased the mucosal recovery due to the expansion of previously induced memory cells.

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A histomorphometric classification system for normal and inflamed mouse duodenum—Quali‐quantitative approach

Cited by 5 publications

References 54 publications

Dietary High Dose of Iron Aggravates the Intestinal Injury but Promotes Intestinal Regeneration by Regulating Intestinal Stem Cells Activity in Adult Mice With Dextran Sodium Sulfate-Induced Colitis

Dietary High Dose of Iron Aggravates the Intestinal Injury but Promotes Intestinal Regeneration by Regulating Intestinal Stem Cells Activity in Adult Mice With Dextran Sodium Sulfate-Induced Colitis

Prior Food-Induced Allergic Gut Inflammation Leading To Multiple Food Allergies in C57Bl/6 Mice

Expansion of Antigen- Specific Memory Cells as a Potential Booster for Food Tolerance Induction

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