A histological study of the developing condylar cartilage of the fetal mouse mandible using coronal sections

Shibata, Shunichi; Suzuki, Shigehiko; Tengan, Toshimoto; Ishii, Masako; Kuroda, Takayuki

doi:10.1016/0003-9969(95)00105-0

Cited by 52 publications

(58 citation statements)

References 10 publications

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“…6) The cells of the central region of the core cell aggregate differentiate into hypertrophic chondrocytes as early as 14.5 dpc. 6,17) The P 1 layer expressed type II collagen in the deep hypertrophic chondrocyte zone as early as 15.0 dpc and, thereafter, its expression was expanded until it covered the whole area of the central region of the core cell aggregate of the anlage by 17.0 dpc. A large number of studies have examined the expression of type II collagen in the mandibular condylar cartilage (MCC).…”

Section: Discussionmentioning

confidence: 99%

An immunohistochemical study on the localization of type II collagen in the developing mouse mandibular condyle

Kenzaki

Tsuchikawa

Kuwahara

2011

Okajimas Folia Anat. Jpn.

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The present chronological investigation assessed the distribution of type II collagen expression in the developing mouse mandibular condyle using immunohistochemical staining with respect to the anatomy of the anlage of the mandibular condyle, the histological characteristics of which were disclosed in our previous investigation. we analyzed fetuses, obtained by cross breeding of Icr strain mice, between 14.0 and 19.0 days post-conception (dpc) and pups on 1, 3, and 5 days post-natal (dpn) using immunohistochemical staining with 2 anti-type II collagen antibodies. The expression of type II collagen was first detected at 15.0 dpc in the lower part of the hypertrophic chondrocyte zone; thereafter, this type II collagen-positive layer was expanded and intensified (P 1 layer). At 17.0 dpc, we identified a type II collagen-negative layer ( n layer) around the P 1 layer and we also identified another newly formed type II collagen-positive layer (P 2 layer) on the outer surface of the n layer. The most typical and conspicuous 3-layered distribution was observed at 1 dpn; thereafter, there was a reduction in the intensity of expression, and with it, the demarcation between the layers was weakened by 5 dpn. The P 1 layer was derived from the central region of the core cell aggregate of the anlage of the mandibular condyle and participated in endochondral bone formation. The n layer was derived from the fringe of the core cell aggregate of the anlage, formed the bone collar at the side of the condyle by intramembranous bone formation, and showed a high level of proliferative activity at the vault. The P 2 layer was formed from the outgrowth of the n layer, and could be considered as the secondary cartilage. The intensive expression of type II collagen from 17.0 dpc to 3 dpn was detected in the fibrous sheath covering the condylar head, which is derived from the peripheral cell aggregate of the anlage. since its expression in the fibrous sheath was not detected in the neighboring section in the absence of hyaluronidase digestion, some changes in the extracellular matrix of the fibrous sheath appear to participate in the generation of the lower joint space. The results of the present investigation indicate that further studies are required to fully characterize the development of the mouse mandibular condyle.

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Section: Discussionmentioning

confidence: 99%

An immunohistochemical study on the localization of type II collagen in the developing mouse mandibular condyle

Kenzaki

Tsuchikawa

Kuwahara

2011

Okajimas Folia Anat. Jpn.

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“…Indeed, mandibular condylar cartilage is derived from ALP-positive progenitor cells of the periosteum that are continuous with the earlierformed ossifying mandible (Shibata et al 1996(Shibata et al , 1997 Miyake et al 1997;Fukada et al 1999;Fukuoka et al 2007). Thus secondary cartilage formation is closely related to the formation of perichondrium ⁄ periosteum.…”

Section: Discussionmentioning

confidence: 99%

“…Mandibular condylar cartilage is a representative mammalian secondary cartilage derived from alkaline phosphatase (ALP)-positive progenitor cells of the periosteum that are continuous with the preliminarily formed ossifying mandible (Shibata et al 1996(Shibata et al , 1997Miyake et al 1997;Fukada et al 1999;Fukuoka et al 2007). Progenitor cells of this cartilage rapidly differentiate into hypertrophic chondrocytes (Shibata et al 1997;Fukada et al 1999;Fukuoka et al 2007), after which the temporomandibular joint, consisting of condylar cartilage, an articular disc, and upper and lower joint cavities, can be morphologically identified by E18.5 (Shibukawa et al 2007).…”

Section: Introductionmentioning

confidence: 99%

Functional analysis of CTRP3/cartducin in Meckel’s cartilage and developing condylar cartilage in the fetal mouse mandible

et al. 2011

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CTRP3 ⁄ cartducin, a novel C1q family protein, is expressed in proliferating chondrocytes in the growth plate and has an important role in regulating the growth of both chondrogenic precursors and chondrocytes in vitro. We examined the expression of CTRP3 ⁄ cartducin mRNA in Meckel's cartilage and in condylar cartilage of the fetal mouse mandible. Based on in situ hybridization studies, CTRP3 ⁄ cartducin mRNA was not expressed in the anlagen of Meckel's cartilage at embryonic day (E)11.5, but it was strongly expressed in Meckel's cartilage at E14.0, and then reduced in the hypertrophic chondrocytes at E16.0. CTRP3 ⁄ cartducin mRNA was not expressed in the condylar anlagen at E14.0, but was expressed in the upper part of newly formed condylar cartilage at E15.0. At E16.0, CTRP3 ⁄ cartducin mRNA was expressed from the polymorphic cell zone to the upper part of the hypertrophic cell zone, but was reduced in the lower part of the hypertrophic cell zone. CTRP3 ⁄ cartducin-antisense oligodeoxynucleotide (AS-ODN) treatment of Meckel's cartilage and condylar anlagen from E14.0 using an organ culture system indicated that, after 4-day culture, CTRP3 ⁄ cartducin abrogation induced curvature deformation of Meckel's cartilage with loss of the perichondrium and new cartilage formation. Aggrecan, type I collagen, and tenascin-C were simultaneously immunostained in this newly formed cartilage, indicating possible transformation from the perichondrium into cartilage. Further, addition of recombinant mouse CTRP3 ⁄ cartducin protein to the organ culture medium with AS-ODN tended to reverse the deformation. These results suggest a novel function for CTRP3 ⁄ cartducin in maintaining the perichondrium. Moreover, AS-ODN induced a deformation of the shape, loss of the perichondrium ⁄ fibrous cell zone, and disorder of the distinct architecture of zones in the mandibular condylar cartilage. Additionally, AS-ODN-treated condylar cartilage showed reduced levels of mRNA expression of aggrecan, collagen types I and X, and reduced BrdU-incorporation. These results suggest that CTRP3 ⁄ cartducin is not only involved in the proliferation and differentiation of chondrocytes, but also contributes to the regulation of mandibular condylar cartilage.

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“…Although the condyle cartilage basically contributes to mandible growth through endochondral bone formation as that of growth plate, it also displays unique features in development and regulation. The condyle cartilage differs to the primary cartilage in embryonic origin, histological organization, growth mode and regulation (Delatte et al, 2004, Shibata et al, 1996.…”

Section: Bmp Signalling In Craniofacial Skeletogenesismentioning

confidence: 99%

BMP signalling in craniofacial development

Nie

Luukko²,

Kettunen³

2006

Int. J. Dev. Biol.

146

130

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A histological study of the developing condylar cartilage of the fetal mouse mandible using coronal sections

Cited by 52 publications

References 10 publications

An immunohistochemical study on the localization of type II collagen in the developing mouse mandibular condyle

An immunohistochemical study on the localization of type II collagen in the developing mouse mandibular condyle

Functional analysis of CTRP3/cartducin in Meckel’s cartilage and developing condylar cartilage in the fetal mouse mandible

BMP signalling in craniofacial development

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