A histological study of testis development and ultrastructural features of spermatogenesis in cultured Acrossocheilus fasciatus

Fu, Su-Yan; Jiang, Jianhu; Yang, Wan-Xi; Zhu, Junquan

doi:10.1016/j.tice.2015.10.005

Cited by 17 publications

(5 citation statements)

References 41 publications

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“…The most distinctive feature of the primary spermatocytes was the presence of synaptonemal complexes, which are protein structures, in the nucleus; they are thought to take part in the homologous chromosome pairing step in meiosis ( 37 , 38 ). This characteristic of primary spermatocytes is also observed in Trahira Hoplias malabaricus ( 39 ), the Manila clam, Ruditapes philippinarum ( 40 ), and Acrossocheilus fasciatus ( 10 ).…”

Section: Discussionmentioning

confidence: 57%

“…Therefore, studying the ultrastructure of spermatozoa and spermatogenic cells during developmental stages may clarify the reproductive mechanism ( 2 , 9 ). The ultrastructure of spermatogenesis and the morphology of mature spermatozoa have been examined in many teleost species ( 10–13 ) using both light and electron microscopy. However, research on the ultrastructural features of teleost spermatogenesis is scarce; a number of studies have described the ultrastructural details of spermatogenic cells in the common carp, Cyprinus carpio ( 14 ), silver pomfret, Pampus argenteus ( 14 ), burbot, Lota lota ( 15 ), rock flounder, Kareius bicoloratus ( 16 ), and grey armored catfish, Liposarcus anisitsi ( 17 ).…”

Section: Introductionmentioning

confidence: 99%

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Fine ultrastructural features of germ cells and spermatozoa during spermatogenesis in the European grayling, Thymallus thymallus (Teleostei, Salmoniformes, Salmonidae)

Dadras,

Imentai

et al. 2023

Front. Vet. Sci.

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This study aimed to examine the ultrastructure of spermatogenic stages and mature spermatozoa in the European grayling, Thymallus thymallus. The testes were examined microscopically with a transmission electron microscope to find out details of the structure and morphology of the grayling germ cells, spermatozoa and some somatic cells. The grayling testis has a tubular shape, with cysts or clusters of germ cells within seminiferous lobules. The spermatogenic cells, including spermatogonia, spermatocytes, and spermatids, can be found along seminiferous tubules. There are electron-dense bodies in germ cells from the primary spermatogonia to secondary spermatocyte stages. These undergo mitosis to reach the secondary spermatogonia stage, when they form primary and secondary spermatocytes. Spermatids undergo three different stages of differentiation during spermiogenesis, characterized by the level of chromatin condensation, elimination of cytoplasm, and the occurrence of the flagellum. The midpiece of spermatozoa is short and contains spherical or ovoid mitochondria. The sperm flagellum has an axoneme with nine doublets of peripheral microtubules and two central microtubules. The result of this study is valuable to be used as a standard reference for germ cell development, which is of great importance to get a clear insight into the process of grayling breeding practice.

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Section: Discussionmentioning

confidence: 57%

Section: Introductionmentioning

confidence: 99%

Fine ultrastructural features of germ cells and spermatozoa during spermatogenesis in the European grayling, Thymallus thymallus (Teleostei, Salmoniformes, Salmonidae)

Dadras,

Imentai

et al. 2023

Front. Vet. Sci.

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“…Gwo et al reported on p.286 that in Spratelloides gracilis, “No proximal centriole was identified” [ 53 ], while it was reported that this species reproduces by external fertilization [ 54 , 55 ].…”

Section: Resultsmentioning

confidence: 99%

Atypical Centriolar Composition Correlates with Internal Fertilization in Fish

Turner

Solanki

Salouha

et al. 2022

Cells

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The sperm competition theory, as proposed by Geoff Parker, predicts that sperm evolve through a cascade of changes. As an example, internal fertilization is followed by sperm morphology diversification. However, little is known about the evolution of internal sperm structures. The centriole has an ancient and evolutionarily conserved canonical structure with signature 9-fold, radially symmetric microtubules that form the cell’s centrosomes, cilia, and flagella. Most animal spermatozoa have two centrioles, one of which forms the spermatozoan flagellum. Both are delivered to the egg and constitute the embryo’s first two centrosomes. The spermatozoa of mammals and insects only have one recognizable centriole with a canonical structure. A second sperm centriole with an atypical structure was recently reported in both animal groups and which, prior to this, eluded discovery by standard techniques and criteria. Because the ancestors of both mammals and insects reproduced by internal fertilization, we hypothesized that the transition from two centrioles with canonical composition in ancestral sperm to an atypical centriolar composition characterized by only one canonical centriole evolved preferentially after internal fertilization. We examined fish because of the diversity of species available to test this hypothesis–as some species reproduce via internal and others via external fertilization–and because their spermatozoan ultrastructure has been extensively studied. Our literature search reports on 277 fish species. Species reported with atypical centriolar composition are specifically enriched among internal fertilizers compared to external fertilizers (7/34, 20.6% versus 2/243, 0.80%; p < 0.00001, odds ratio = 32.4) and represent phylogenetically unrelated fish. Atypical centrioles are present in the internal fertilizers of the subfamily Poeciliinae. Therefore, internally fertilizing fish preferentially and independently evolved spermatozoa with atypical centriolar composition multiple times, agreeing with Parker’s cascade theory.

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“…In addition, it’s flesh is tender, delicious and contains highly polyunsaturated fatty acids (PUFA), possessing a considerable market value. Recently, the freshwater grouper A. fasciatus has become a commercially emerging aquaculture fish due to its nutritive and ornamental value 3 . Moreover, as an omnivorous fish, the growth of A. fasciatus requires to feed with moss and other algae plants, which can inhibit the rankness of these aquatic plants, thus playing a role in ecological balance.…”

Section: Background and Summarymentioning

confidence: 99%

Chromosome-level genome assembly of Acrossocheilus fasciatus using PacBio sequencing and Hi-C technology

Zheng,

Jiang,

Rui

et al. 2024

Sci Data

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Acrossocheilus fasciatus (Cypriniformes, Cyprinidae) is emerged as a newly commercial stream fish in the south of China with high economic and ornamental value. In this study, a chromosome-level reference genome of A. fasciatus was assembled using PacBio, Illumina and Hi-C sequencing technologies. As a result, a high-quality genome was generated with a size of 879.52 Mb (accession number: JAVLVS000000000), scaffold N50 of 32.7 Mb, and contig N50 of 32.7 Mb. The largest and smallest scafford was 60.57 Mb and 16 kb, respectively. BUSCO analysis showed a completeness score of 98.3%. Meanwhile, the assembled sequences were anchored to 25 pseudo-chromosomes with an integration efficiency of 96.95%. Additionally, we found approximately 390.91 Mb of repetitive sequences that accounting for 44.45% of the assembled genome, and predicted 24,900 protein-coding genes. The available genome reported in the present study provided a crucial resource to further investigate the regulation mechanism of genetic diversity, sexual dimorphism and evolutionary histories.

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A histological study of testis development and ultrastructural features of spermatogenesis in cultured Acrossocheilus fasciatus

Cited by 17 publications

References 41 publications

Fine ultrastructural features of germ cells and spermatozoa during spermatogenesis in the European grayling, Thymallus thymallus (Teleostei, Salmoniformes, Salmonidae)

Fine ultrastructural features of germ cells and spermatozoa during spermatogenesis in the European grayling, Thymallus thymallus (Teleostei, Salmoniformes, Salmonidae)

Atypical Centriolar Composition Correlates with Internal Fertilization in Fish

Chromosome-level genome assembly of Acrossocheilus fasciatus using PacBio sequencing and Hi-C technology

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