A histological study of mineralised tissue formation around implants with 3D culture of HMS0014 cells in Cellmatrix Type I-A collagen gel scaffold &lt;i&gt;in vitro&lt;/i&gt;

Morishita, Aiko; Kumabe, Shunji; Nakatsuka, Michiko; Iwai, Yoshio

doi:10.2535/ofaj.91.57

Cited by 2 publications

(3 citation statements)

References 63 publications

(76 reference statements)

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“…As shown in Figure 1 , we evaluated the performance of the gel and the Neu embedded in it. Cellmatrix type I collagen has been used to culture mesenchymal stem cells by other researchers before ( 27 ). We examined the characteristics of the four collagen gels before exploring the role of collagen gels in the cultivation of Neu.…”

Section: Resultsmentioning

confidence: 99%

Neutrophils Culture in Collagen Gel System

Wang

Huang

et al. 2022

Front. Immunol.

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Neutrophils (Neu) migrate rapidly to damaged tissue and play critical roles in host defense and tissue homeostasis, including the intestinal epithelia injuries and immune responses. Although their important roles in these diseases, they are challenging to study due to their short life span and the inability to cryopreserve or expand them in vitro. Moreover, the standard cell culturing on plastic plates (two-dimensional (2D) cultures) does not represent the actual microenvironment where cells reside in tissues. In this study, we developed a new three-dimensional (3D) culture system for human and mouse peripheral blood Neu, which is made of hydrogel. The Neu showed much better cell integrity and less cell debris in the 3D culture system compared to that in 2D culture system. Moreover, the 3D culture system was more suitable for the observation of neutrophil extracellular traps (NETs) stimulated by the classical stimulation phorbol ester (PMA), and other damage associated molecular patterns (DAMPs) such as Lipopolysaccharide (LPS)/ATP, interleukin-1 β (IL-1β) and tumor necrosis factor α (TNFα) than the 2D culture system. Moreover, NETs phenomenon in 3D culture system is similar to that in vivo. In addition, the 3D culture system was evaluated to co-culturing Neu and other parenchymal cells, such as colon mucosal epithelial cell lines. In conclusion, the 3D culture system could maintain better properties of Neu than that in 2D culture system and it may reduce the gap between in vitro an in vivo experimentations.

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Section: Resultsmentioning

confidence: 99%

Neutrophils Culture in Collagen Gel System

Wang

Huang

et al. 2022

Front. Immunol.

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“…Their study showed that collagen composites were able to enhance stiffness and porosity for cell penetration, thus providing a favorable scaffold for cell culture. Moreover, several studies have provided evidence that 3D cell cultivation improves the differentiation in embryonic stem cell-derived bone progenitors in a nano-fibrous scaffold that can maintain 3D cell morphology and pluripotency, promoting BSP and OC mRNA expression after 4 weeks of cultivation (18)(19)(20).…”

Section: Discussionmentioning

confidence: 99%

“…PDLSCs at the third to fifth passages were used in all experiments. To provide a static microenvironment for cell culture, we used a scaffold of collagen type I-A gel for collagen gel-embedded culture, creating an in vitro 3D structure to promote homogeneous osteogenic differentiation, as modified from a previous study (20). CHP-HA or NanoHA was dissolved in GM at a concentration of 1 mg/mL followed by sonication for 10 min and mixing with PDLSCs and collagen gel.…”

Section: Three-dimensional (3d) Collagen Gel-embedded Culturementioning

confidence: 99%

Cellular responses of periodontal ligament stem cells to a novel synthesized form of calcium hydrogen phosphate with a hydroxyapatite-like surface for periodontal tissue engineering

et al. 2018

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Calcium hydrogen phosphate with a hydroxyapatite-like surface (CHP-HA) is a novel synthesized compound designed to overcome the limitations of bioactive ceramics. It was originally applied as nano-sized HA strips covering core plates to enhance the degree of interfacial attachment. The objective of the present study was to examine the cellular attachment, proliferation, and osteogenic differentiation of periodontal ligament stem cells (PDLSCs) on a CHP-HA substrate in comparison with conventional nanohydroxyapatite (NanoHA). The PDLSCs were cultivated with either CHP-HA or NanoHA for cellular attachment, proliferation, and osteogenic differentiation assay. Osteogenic differentiation was examined using quantitative polymerase chain reaction and immunofluorescence after confirmation by Alizarin red staining. We found that between 14 and 21 days, CHP-HA exhibited a well-organized matrix distribution, a high degree of cell proliferation, and a high level of Alizarin red staining in comparison to NanoHA. Expression of all the osteogenic markers examined was increased significantly relative to NanoHA at 14 days, but no significant differences in some osteogenic genes were found at 21 days. Immunofluorescence revealed stronger staining in the CHP-HA group. In conclusion, PDLSCs cultivated with this novel CHP-HA show enhanced cellular responses. We propose that CHP-HA may be a promising alternative biomaterial for periodontal tissue engineering.

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A histological study of mineralised tissue formation around implants with 3D culture of HMS0014 cells in Cellmatrix Type I-A collagen gel scaffold <i>in vitro</i>

Cited by 2 publications

References 63 publications

Neutrophils Culture in Collagen Gel System

Neutrophils Culture in Collagen Gel System

Cellular responses of periodontal ligament stem cells to a novel synthesized form of calcium hydrogen phosphate with a hydroxyapatite-like surface for periodontal tissue engineering

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