A Histological Study of Anodic Oxide Titanium Implant after Hydrothermal Treatment.

Kajimura, Kouichi; Shioyama, Tsukasa; Yamamori, Tetsuo; Itoh, Sozo; Hosokawa, Mitsugu; Shimazaki, Nobuko; Arizumi, Tatsuya; Ishibashi, Kanji; Ishizawa, Hitoshi

doi:10.2186/jjps.40.946

Cited by 14 publications

(12 citation statements)

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“…It was anticipated that anodization and hydrothermal modification of the topographically enhanced cpTi surface would influence bone formation by modulation of cellular activities at relatively early stages in wound healing. It has recently been confirmed that marked increases in bone‐to‐endosseous implant contact occur at HA/cpTi endosseous implants after 4 weeks in the mandibles of beagles 13, 16. Surface topography and physicochemical properties have been suggested to positively influence bone formation at the early stages of wound healing and blood clot formation and retention 38.…”

Section: Discussionmentioning

confidence: 93%

“…It has recently been confirmed that marked increases in boneto-endosseous implant contact occur at HA/cpTi endosseous implants after 4 weeks in the mandibles of bea-gles. 13,16 Surface topography and physicochemical properties have been suggested to positively influence bone formation at the early stages of wound healing and blood clot formation and retention. 38 In this study, chemical modification of a topographically enhanced surface maintained the rough surface topography and the associated advantages on the early stages of healing that promote contact osseointegration.…”

Section: Discussionmentioning

confidence: 99%

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Anodic oxidation and hydrothermal treatment of commercially pure titanium surfaces increases expression of bone morphogenetic protein‐2 in the adherent macrophage cell line J774A.1

Takebe

Ito

Champagne

et al. 2007

J Biomedical Materials Res

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The surface property of commercially pure titanium (cpTi) was improved by forming a thin hydroxyapatite (HA) layer by anodic oxidation and hydrothermal treatment (HA/cpTi). We hypothesize that the adhesion of macrophages to HA/cpTi surfaces is important to the process of osseointegration. This study investigates the effect of adhesion of macrophages to HA/cpTi surfaces on the expression of bone morphogenetic protein-2 (BMP-2). The murine macrophage cell line J774A.1 was cultured on HA/cpTi and polished cpTi (S/cpTi). Macrophage cell adhesion was examined by SEM, 0-72 h following plating onto HA/cpTi and S/cpTi. BMP-2 gene expression was examined by RT-PCR analysis. The level of BMP-2 secreted into the supernatant was measured using an ELISA assay. The extent of macrophage adhesion increased with time on both the HA/cpTi and S/cpTi surfaces, with a" higher degree of spreading observed on HA/cpTi than onS/cpTi surfaces after 24 or 72 h. The ratio of BMP-2 mRNA was higher on HA/cpTi than on S/cpTi after 24 h (0.348 vs. 0, p < 0.05) and 72 h (0.584 vs. 0.189, p < 0.05). After 24 h, secretion of BMP-2 was detected in cultures grown on HA/cpTi, but not on S/cpTi. After 72 h, secretion of BMP-2 was detected in cultures grown on S/cpTi, but the levels were higher in cultures grown on HA/cpTi. These findings show that macrophages have the capacity to adhere to HA/cpTi endosseous implants and provide a source of osteoinductive cytokines that may play a key role in the process of osseointegration.

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Section: Discussionmentioning

confidence: 93%

Section: Discussionmentioning

confidence: 99%

Anodic oxidation and hydrothermal treatment of commercially pure titanium surfaces increases expression of bone morphogenetic protein‐2 in the adherent macrophage cell line J774A.1

Takebe

Ito

Champagne

et al. 2007

J Biomedical Materials Res

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“…This in vivo study suggested that HA/Ti implant materials have more direct contact with its surrounding bone tissue than nontreated Ti implant materials, and that SA treatment increases the compatibility of Ti surfaces. 17 Furthermore, plasmasprayed HA-coated implant materials show excoriation and bioresorption of HA films in contrast to HA/ Ti implant materials. When HA/Ti implant materials were compared with the plasma-sprayed HA-coated implant materials, similar results were found in relation to the bone tissue compatibility.…”

Section: Discussionmentioning

confidence: 99%

The effect on immunocytes of anodic oxide titanium after hydrothermal treatment

Takebe

Itoh

Ariake

et al. 1998

J. Biomed. Mater. Res.

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All dental root implants come in contact with the oral epithelium, and many complex factors are found to arise in this region. In order to perform a successful dental root implantation, it is necessary to clarify the interaction of the dental root implant material with the host defense mechanisms involved in the specific and nonspecific immune responses to many antigens in oral bacteria and their components. Recently, focusing on developing the dental root implant, the Nikon Corporation improved the surface characteristics of pure titanium even further by developing a hydroxyapatite (HA) layer formed on an anodic titanium oxide film containing Ca and P via hydrothermal treatment (SA treatment). However, since little is known about the effect of SA-treated pure titanium (HA/Ti) on the defense mechanisms of the oral membrane epithelium, we investigated (1) the in vitro proliferation of murine splenic B lymphocytes on the surface of HA/Ti in the presence of three lipopolysaccharide (LPS) concentrations and (2) interleukin-1alpha (IL-1alpha) production by the reaction of human peripheral blood mononuclear cells (PBM cells) on the surface of HA/Ti under the same concentrations. After culture, murine splenic lymphocytes were measured by uptake of 3H-thymidine, and cytokine release (IL-1alpha) from PBM cells was measured by ELISA. Results showed that HA/Ti had hardly any effect on the LPS-induced proliferation of B lymphocytes and IL-1alpha production. In vitro investigations of the effects of HA/Ti on the LPS-induced proliferation of murine splenic B lymphocytes and IL-1alpha from PBM cells might be a useful way of elucidating the defense mechanism between implants and the oral epithelium.

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“…17,28,46,47 Efforts are being made to improve the bioactivity of titanium implants using different methods. By calcium-ion implantation, 56 by alkali treatment, 57 or by developing a thin HA film on a thick porous anodic oxide film on titanium, 58,59 it has been reported that the bioactivity of titanium can be improved, as evidenced by enhanced bone-like apatite formation on titanium surfaces. The surface hydroxyl groups on the oxide have been suggested as playing an important role in the improvement.…”

Section: Possibility Of Improving Bioactivity Of Titanium Implantsmentioning

confidence: 99%

Variation of oxide films on titanium induced by osteoblast-like cell culture and the influence of an H2O2 pretreatment

Pan

Liao

Leygraf

et al. 1998

J. Biomed. Mater. Res.

163

130

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Variations of titanium oxide films induced by osteoblast-like cells in a rat calvaria culture system and the influence of an H2O2 pretreatment have been investigated by using X-ray photoelectron spectroscopy and electrochemical impedance spectroscopy. For abraded titanium, the results revealed that phosphate and calcium ions may incorporate into the surface oxide film during the cell culture, forming a precipitate with a Ca/P ratio near that of hydroxyapatite. Oxidized carbon also was found in the surface layer, most likely precipitated hydroxylcarbonated apatite (HCA). The H2O2 pretreatment of titanium in a phosphate-buffered saline solution results in a 10-fold thickened porous oxide film and large amounts of surface hydroxyl groups as well as a certain amount of phosphate ions inside the oxide film. During the cell culture, the H2O2-treated titanium surface favors the ion incorporation and precipitation of the HCA-like compound, which probably is inlaid into the oxide film. Osteoblast-like cells on the H2O2-treated titanium showed a more active morphology during the initial stage compared with cells on abraded titanium. Moreover, bone-like nodule formation and mineralization appear to be related to the precipitation of the HCA-like compound on the surface. The results are discussed with respect to corrosion resistance, ion incorporation and precipitation of the HCA-like compound on the surface, osseointegration, and bioactivity of titanium implants.

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A Histological Study of Anodic Oxide Titanium Implant after Hydrothermal Treatment.

Cited by 14 publications

References 0 publications

Anodic oxidation and hydrothermal treatment of commercially pure titanium surfaces increases expression of bone morphogenetic protein‐2 in the adherent macrophage cell line J774A.1

Anodic oxidation and hydrothermal treatment of commercially pure titanium surfaces increases expression of bone morphogenetic protein‐2 in the adherent macrophage cell line J774A.1

The effect on immunocytes of anodic oxide titanium after hydrothermal treatment

Variation of oxide films on titanium induced by osteoblast-like cell culture and the influence of an H2O2 pretreatment

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