A Histological, Immunological, and Electron Microscopic Study of Bovine Collagen Implants in the Human

Burke, Karen E.; Naughton, Gail K.; Cassai, Nick

doi:10.1097/00000637-198506000-00004

Cited by 64 publications

(30 citation statements)

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“…This precise effect has been recently demonstrated by experiments in which intentionally differentially loaded IVDs (half of the disc in compression and half in tension) in a disc degeneration model (Lotz et al 2008). There are numerous other phenomena where a mechanochemical relationship between collagen and MMP may have relevance, including the instability of unloaded collagen implants (Burke et al 1983(Burke et al , 1985Donald 1986) and the documented loss of bone and ligament strength in microgravity (Vailas et al 1990;Zerath 1998). It is also interesting to consider exactly why fibroblasts, upon entering a randomly self-assembled collagen gel, contract the matrix (Bell et al 1979).…”

Section: Phil Trans R Soc a (2009)mentioning

confidence: 91%

Mechanical strain enhances survivability of collagen micronetworks in the presence of collagenase: implications for load-bearing matrix growth and stability

Bhole

Flynn

Liles

et al. 2009

Phil. Trans. R. Soc. A.

118

109

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There has been great interest in understanding the methods by which collagen-based load-bearing tissue is constructed, grown and maintained in vertebrate animals. To date, the responsibility for this process has largely been placed with mesenchymal fibroblastic cells that are thought to fully control the morphology of load-bearing extracellular matrix (ECM). However, given clear limitations in the ability of fibroblastic cells to precisely place or remove single collagen molecules to sculpt tissue, we have hypothesized that the material itself must play a critical role in the determination of the form of structural ECM. We here demonstrate directly, using live, dynamic, differential interference contrast imaging, that mechanically strained networks of collagen fibrils, exposed to collagenase (Clostridium histolyticum), degrade preferentially. Specifically, unstrained fibrils are removed 'quickly', while strained fibrils persist significantly longer. The demonstration supports the idea that collagen networks are mechanosensitive in that they are stabilized by mechanical strain. Thus, collagen molecules (together with their complement enzymes) may comprise the basis of a smart, load-adaptive, structural material system. This concept has the potential to drastically simplify the assumed role of the fibroblast, which would need only to provide ECM molecules and mechanical force to sculpt collagenous tissue.

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Section: Phil Trans R Soc a (2009)mentioning

confidence: 91%

Mechanical strain enhances survivability of collagen micronetworks in the presence of collagenase: implications for load-bearing matrix growth and stability

Bhole

Flynn

Liles

et al. 2009

Phil. Trans. R. Soc. A.

118

109

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“…Collagen gels have not been previously used for injectable for cartilage tissue engineering, but have been proven as a useful injectable dermal filler, 10,11 and have been proposed as an injectable for other tissue engineering applications. [12][13][14][15] Type I collagen gels have been shown to be a viable scaffold for tissue-engineered cartilage constructs grown in vitro and when implanted into an in vivo surface, focal, and critical size defects in rabbit articular cartilage. [16][17][18][19] The main limitation of collagen gels as a scaffold for the tissue engineering of cartilage is that they may not provide sufficient structural support due to their lack of stiffness.…”

mentioning

confidence: 99%

Non‐enzymatic glycation of chondrocyte‐seeded collagen gels for cartilage tissue engineering

Roy¹,

Boskey²,

Bonassar³

2008

Journal Orthopaedic Research

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Collagen glycated with ribose (250 mM) in solution (pre-glycation) and as a gel (post-glycation) was seeded with chondrocytes and the effects of glycation on chondrocyte matrix assembly in culture were determined. Pre-glycation enhanced GAG accumulation significantly over controls at both 2 and 4 weeks (p < 0.05), although at both time points there were no statistical differences in cell number between pre-glycated and control gels. The increased proteoglycan accumulation was shown to be in part due to significantly increased GAG retention by the pre-glycated constructs (p < 0.05). Total collagen content in these pre-glycated gels was also significantly higher than unglycated gels at 4 weeks (p < 0.05). With post-glycation of collagen gels, chondrocyte number and GAG accumulation were all significantly lower than controls (p < 0.05). Post-glycation also inhibited GAG retention by the constructs (p < 0.05). Given these results, pre-glycation may be an improved processing method for collagen gels for tissue engineering techniques. ß

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“…The formation of this neodermis occurs within 14-21 days. After the completion of regeneration, the neodermis is histologically and functionally similar to normal dermis [98,99]. This dermal substitute is considered to be an effective treatment for burns and tissue reconstruction in patients with cancer [100,101].…”

Section: Tissue Engineering 238mentioning

confidence: 99%

Bioresorbable Polymers for Tissue Engineering

Santos¹

2010

Tissue Engineering

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Polymeric biomaterials are used as substitutes for damaged tissue and for the stimulation of tissue regeneration. One class of polymeric biomaterials are bioresorbable polymers that degrade both in vitro and in vivo and are used as a temporary support for tissue regeneration. Among the various types of bioresorbable polymers, -hydroxy acids including the different forms of poly(lactic acid) (PLA), such as poly(L-lactic acid), poly(Dlactic acid) and poly(DL-lactic acid), as well as poly(glycolic acid) and polycaprolactone, have been extensively studied. These polymers are well known for their good biocompatibility, with their degradation products being eliminated from the body by metabolic pathways. Many reports have shown that the different PLA-based substrates do not present toxicity since the cells were found to differentiate over the different polymers, as demonstrated by the production of extracellular matrix components by various cell types. In this chapter, we describe the use of -hydroxy acids, highlighting the different forms of PLA scaffolds used as cell culture substrates and their applications in clinical practice. The chapter is divided into (1) Introduction; (2) Bioresorbable devices as cell culture substrates; (3) Cell adhesion to polymer substrates; (4) Tissue engineering and bioresorbable polymers; (5) Cell growth and proliferation on bioresorbable polymers; (6) Bioresorbable polymers for cartilage engineering; (7) Bioresorbable polymers for bone tissue engineering; (8) Bioresorbable polymers for skin tissue engineering, and (9) Conclusion.

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A Histological, Immunological, and Electron Microscopic Study of Bovine Collagen Implants in the Human

Cited by 64 publications

References 0 publications

Mechanical strain enhances survivability of collagen micronetworks in the presence of collagenase: implications for load-bearing matrix growth and stability

Mechanical strain enhances survivability of collagen micronetworks in the presence of collagenase: implications for load-bearing matrix growth and stability

Non‐enzymatic glycation of chondrocyte‐seeded collagen gels for cartilage tissue engineering

Bioresorbable Polymers for Tissue Engineering

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