A histochemical study of long bones in osteogenesis imperfecta congenita

Abstract

Stewart, Robert B. (University of Rochester, Rochester, N. Y.). Growth and lethal effects of the psittacosis agent in chicken embryos of different ages. J. Bacteriol. 83:423-428. 1962.-Inoculation of the psittacosis agent (strain 6BC) into the yolk sacs of chicken eggs in different stages of embryonic development resulted in a steady decrease in ld(50) titer as the embryos increased in age from 7 to 10 days. Correlated with the decreased ld(50) titers in older eggs was the finding that the titers of the organism were lower in the yolk-sac tissues of the older eggs. The embryos themselves also gained appreciably in resistance with increase in age, since comparable titers of psittacosis organisms were found in embryonic tissues of differing ages at the time of inoculation but the mortality of older embryos was considerably less. This resistance of older embryos to the lethal effects of the psittacosis agent could not be demonstrated when the organism was introduced intravenously instead of into the yolk sac.

In vitro sulfate turnover in osteogenesis imperfecta congenita and tarda

“…structural observations suggest either a defect of the bone matrix involving collagen [25] or a functional impairment of osteoblastic activity [6]. In 1962 Spencer [24] had histochemical evidence for the presence of abnormally staining proteoglycans in long bones of one case of osteogenesis imperfecta with multiple fractures at birthprobably lethal perinatal 01. Furthermore, Solheini in 1969 [21], extracted over-sulfated chondroitin sulfates from long bones of one case of osteogenesis imperfecta "tarda."…”

Altered glycosaminoglycan production in cultured osteogenesis-imperfecta skin fibroblasts

“…Several other authors have reported alterations in glycosaminoglycans in 01 (Spencer, 1962;Solheim, 1969;Bleckmann et al, 1971;Engfeldt & Hjerpe, 1976;Cetta et al, 1977). An increase in the total amounts of glycosaminoglycans in OI bone and cartilage (Spencer, 1962;Bleckmann et al, 1971) and in their sulphation (Engfeldt & Hjerpe, 1976) has been reported. However, a low chondroitin sulphate content has been recorded in some bone and cartilage specimens (Solheim, 1969;Cetta et al, 1977).…”

Histology and Histochemistry of the Long Bones in Micromelia

“…There is evidence that an increase in acid mucopolysaccharide may inhibit the crystal nucleation process of ossification (Neuman, 1960;Spencer, 1962).…”