A histochemical identification of the androgen-producing cells in the gonads of the domestic fowl and albino rat

Woods, Jennifer; Domm, L. V.

doi:10.1016/0016-6480(66)90076-1

Cited by 88 publications

(14 citation statements)

References 34 publications

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“…This suggests an interference with the functional activity of the Sertoli cells, reminiscent of cadmium damage (Lofts & Murtón, 1967). Since the mammalian (Lacy, Lofts, Kinson, Hopkins & Dott, 1965) and fowl (Woods & Domm, 1966) Sertoli cell appears to be capable of steroid synthesis, it might be expected that such an anti-cholesterol agent would interfere with its functional activity and this is supported by the present results. Endogenous cholesterol production by the avian seminiferous tubules is very extensive at certain times of year (Marshall, 1955 …”

supporting

confidence: 77%

The Effects of 22,25-Diazacholesterol Dihydrochloride on the Pigeon Testis and on Reproductive Behaviour

Lofts¹,

Murton²,

Thearle³

1968

Reproduction

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In recent years, attention has been focused on the potential of reproduction inhibitors for the possible control of harmful birds (Davis, 1961(Davis, , 1962 Elder, 1964;Becker, 1966). Elder, in his search for an effective avian oral contraceptive, found that the best results were obtained with a hypocholesterolemic agent, 22,25-diazacholesterol dihydrochloride (compound SC-12937, Searle & Co., Chicago). When this constituted 0\m=.\1% of the diet of pigeons for 10 days, ovulation was inhibited for 3 months and impaired for a further 3 to 9 months. The present study was designed to investigate the effects of compound SC-12937 on the pre-incubatory behaviour of Columba livia and to assess the action of the drug on normal testis cytology.Males and females in two groups, each of three pairs, and a third group of six pairs, were laparotomized, their gonads measured and seen to be in breeding condition. The individuals were kept in separate cages for a 3-day post-operative recovery period. The members of each pair were then allowed access to each other for 15 min to ensure that the males would all give the initial bowing display. Group 1 was then dosed with 30 mg SC-12937, administered in gelatin capsules, for 5 consecutive days during the 1st week and for 5 days more during the 2nd week. Group 2 birds similarly received five doses of 60 mg/bird in the 1st week, but at this point two birds (one male, one female) died from the effects of the drug and the remaining two pairs were given only two further doses during the 2nd week. The total dose/bird for each group was, therefore, 300 and 420 mg, respectively. Group 3 (six pairs) served as controls and were given only empty gelatin capsules. Observations on the behaviour of all birds began at the start of the 2nd week and continued for a week after dosing had ceased. The method used was that adopted by Fabricius & Jansson (1963), based on the techniques of Carpenter (1933) and Beach (1951), in which the birds were housed in individual cages and were only paired for \ hr daily. During this time a record was kept of certain activities and displays occurring in each 2-min period throughout the \ hr and also the frequency with which particular displays followed each other. However, on Days 6 and 7 the birds 145

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supporting

confidence: 77%

The Effects of 22,25-Diazacholesterol Dihydrochloride on the Pigeon Testis and on Reproductive Behaviour

Lofts¹,

Murton²,

Thearle³

1968

Reproduction

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“…The localization of testosterone has been detected in frozen and paraffin testicular sections of domestic fowl, rat, squirrel monkey and rainbow trout [2,11,12,15] in biochemical and immunohistochemical studies. Although antisera to steroid hormones has repeatedly been used in immunohisto-or cytochemical study, their suitability is a matter of discussion [3,13] regarding information provided by the staining.…”

mentioning

confidence: 99%

Comparison of the Effects of Two Fixatives for Immunolocalization of Testosterone in the Testes of the Cynomolgus Monkey, Mouse and Rat.

et al. 2000

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Abstract:We compared the effect of two fixatives, Bouin's fixative and neutralized buffered 4% formaldehyde (10% formalin), for immunolocalization of testosterone in the testes of cynomolgus monkeys, mice and rats. In the samples fixed with Bouin's fixative, immunoreactive testosterone was detected as intense deposits in the cytoplasm of Leydig cells of monkeys and mice. Immunoreactive testosterone was detected not only in Leydig cells of rats but also moderately shown within tubules. Immunoreactive testosterone could not be detected in the testes of monkeys, mice or rats fixed with neutralized buffered formalin because of the poor morphology caused by the fixative. It is concluded that Bouin's fixative is a suitable fixative for immunolocalization of testosterone in the testes of cynomolgus monkeys, mice and rats.

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“…In the literature various immunocytochemical methods have been reported (14,18,24). However, as yet, no adequate immunocytochemical technique has been reported.…”

Section: Discussionmentioning

confidence: 99%

Immunocytochemical localization of catecholestrogen in the rat liver after using various fixatives.

Inoue

Yoshizawa

1985

Acta Histochem. Cytochem

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A histochemical identification of the androgen-producing cells in the gonads of the domestic fowl and albino rat

Cited by 88 publications

References 34 publications

The Effects of 22,25-Diazacholesterol Dihydrochloride on the Pigeon Testis and on Reproductive Behaviour

The Effects of 22,25-Diazacholesterol Dihydrochloride on the Pigeon Testis and on Reproductive Behaviour

Comparison of the Effects of Two Fixatives for Immunolocalization of Testosterone in the Testes of the Cynomolgus Monkey, Mouse and Rat.

Immunocytochemical localization of catecholestrogen in the rat liver after using various fixatives.

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