A highly sensitive “switch-on” fluorescent probe for protein quantification and visualization based on aggregation-induced emission

Wang, Fangfang; Wen, Jiying; Huang, Lingyun; Huang, Jinjiu; Ouyang, Jin

doi:10.1039/c2cc33172a

Cited by 70 publications

(52 citation statements)

References 38 publications

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“…However, it is difficult to make the hydrophobic luminophores hydrophilic with unchangeable functions. In addition, the water-soluble probes in solution also exhibit some inborn shortcomings, impeding their extensive applications: (1) the solution-based luminescent platform is inconvenient to store and transport; 18,19 (2) the water-soluble probes cannot be recycled and used because they are not easily separated from the analytes in solution; [20][21][22] (3) the random disposal of these one-off probes leads to the reagent consumption and environmental pollution; 23,24 (4) for the biological samples (e.g., cells and tissue), the water-soluble probes might induce unexpected chemicals released from biological samples; 25 and (5) the water-soluble probes are difficult to be used for vapor/gas detection. On the other hand, the development of inorganic luminophores with water-soluble ligands, such as quantum dots (QDs) [13][14][15] and luminescent metal nanoclusters (NCs), 16,17 seems to be an alternative method but encounters new problems (e.g., limited variety).…”

Section: Introductionmentioning

confidence: 99%

Luminescent films for chemo- and biosensing

et al. 2015

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Luminescent films have received great interest for chemo-/bio-sensing applications due to their distinct advantages over solution-based probes, such as good stability and portability, tunable shape and size, non-invasion, real-time detection, extensive suitability in gas/vapor sensing, and recycling. On the other hand, they can achieve selective and sensitive detection of chemical/biological species using special luminophores with a recognition moiety or the assembly of common luminophores and functional materials. Nowadays, the extensively used assembly techniques include drop-casting/spin-coating, Langmuir-Blodgett (LB), self-assembled monolayers (SAMs), layer-by-layer (LBL), and electrospinning. Therefore, this review summarizes the recent advances in luminescent films with these assembly techniques and their applications in chemo-/bio-sensing. We mainly focused on the discussion of the relationship between the sensing properties of the films and their architecture. Furthermore, we discussed some critical challenges existing in this field and possible solutions that have been or are being developed to overcome these challenges.

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Section: Introductionmentioning

confidence: 99%

Luminescent films for chemo- and biosensing

et al. 2015

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“…The nanoribbons were found to interact with the protein and induce conformational changes to its secondary structure, including the α‐helix content, that unfolded the ferritin protein and disrupted the emissive nanoribbons. The turn‐off detection of ferritin seemed to be instant and gave a good LOD of up to 0.33 ng mL −1 ; this is superior to the AIE‐based light‐up method (0.78 ng mL −1 ) . Detection was selective and showed negligible responses to a range of other metalloproteins.…”

Section: Detection Localization and Quantification Of Proteinsmentioning

confidence: 99%

Fluorogenic Detection and Characterization of Proteins by Aggregation‐Induced Emission Methods

Xie

Wong

Chen

et al. 2019

Chemistry A European J

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Protein is one of the four most important biomacromolecules in living systems. The detection, quantification, localization, and characterization of proteins is essential for an understanding of biological fundamentals, as well as for the diagnostics and treatment of protein‐related diseases. By using intrinsic and extrinsic fluorescence, different techniques have been established to study proteins, many of which are now being routinely used in research laboratories and clinics. This review summarizes the applications of aggregation‐induced emission (AIE) fluorescence in protein science. In contrast to traditional fluorescent dyes, the activation of AIE dyes is mainly attributed to the restriction of intramolecular motions. This unique turn‐on mechanism of AIE dyes allows researchers to develop novel fluorogenic strategies for sensitive, selective, and reliable analysis of proteins. This review focuses on introducing AIE strategies for 1) detection, localization, and quantification of proteins; 2) probing polymer conformational transitions of proteins; 3) characterization of protein–ligand interactions; and 4) evaluation of enzyme activities. Perspectives and challenges with respect to this emerging field of protein characterization are offered.

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“…Recently we discovered an AIE-active molecule, 1,2-Bis [4-(3sulfonatopropoxyl) phenyl]-1,2-diphenylethene salt (BSPOTPE), for staining live particles [24]. BSPOTPE is a water-soluble and biocompatible fluorogen without fluorescence in physiological buffers.…”

Section: Microalgae Can Be a Valuable Indicator In Water Pollution Momentioning

confidence: 99%

Aggregation-induced emission fluorogens as biomarkers to assess the viability of microalgae in aquatic ecosystems

et al. 2015

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Microalgae can be a valuable indicator for monitoring water pollution due to their sensitivity to the changes induced by pollutants in the environment. In this study, an aggregation-induced emission fluorogen was used as a novel tool to differentiate dead and live microalgae and quantify the link between live algal concentration and fluorogen intensity. Protein in the cell protoplasm is the key component contributing to fluorescence emission in algae.

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A highly sensitive “switch-on” fluorescent probe for protein quantification and visualization based on aggregation-induced emission

Cited by 70 publications

References 38 publications

Luminescent films for chemo- and biosensing

Luminescent films for chemo- and biosensing

Fluorogenic Detection and Characterization of Proteins by Aggregation‐Induced Emission Methods

Aggregation-induced emission fluorogens as biomarkers to assess the viability of microalgae in aquatic ecosystems

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