A highly sensitive silver stain for detecting proteins and peptides in polyacrylamide gels

Switzer, Robert C.; Merril, Carl R.; Shifrin, Sidney

doi:10.1016/0003-2697(79)90732-2

Cited by 1,026 publications

(373 citation statements)

References 8 publications

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“…Silver staining with diamine or nitrate was carried out according to the methods [16][17][18][19][20]. The SYPRO Ruby staining procedure is described in Section 2.2.…”

Section: -D Electrophoresismentioning

confidence: 99%

A fluorescent natural product for ultra sensitive detection of proteins in one‐dimensional and two‐dimensional gel electrophoresis

et al. 2003

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Lightning Fast is a sensitive fluorescence-based stain for detecting proteins in onedimensional and two-dimensional polyacrylamide electrophoresis gels. It contains the fluorophore epicocconone from the fungus Epicoccum nigrum that interacts noncovalently with sodium dodecyl sulfate and protein. Stained proteins can be excited optimally by near-ultraviolet light of about 395 nm or with visible light of about 520 nm. The stain can be excited using a range of sources used in image analysis systems including UVA (ca. 365 nm) and UVB (ca. 302 nm) transilluminators; Xenon-arc lamps; 488 nm and 457 nm Argon-ion lasers; 473 nm and 532 nm neodymium: yttrium aluminum garnet (Nd:YAG) solid-state lasers; 543 nm helium-neon lasers, and emerging violet, blue and green diode lasers. Maximum fluorescence emission of the dye is at approximately 610 nm. The limit of detection in one-dimensional gels stained with Lightning Fast protein gel stain is less than 100 pg of protein, rivaling the current limits of matrix-assisted laser desorption/ionization-mass spectrometry (MALDI-MS). Lightning Fast was found to be considerably more sensitive than SYPRO Ruby, SYPRO Orange, silver and Coomassie Brilliant Blue G-250 in matched experiments. Staining takes as little as 3.5 h and stained proteins displayed quantitative linearity over more than four orders of magnitude, thereby allowing visualization of entire proteomes. Lightning Fast protein gel staining is compatible with subsequent peptide mass fingerprinting using MALDI-MS and Edman-based sequencing chemistry.

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“…Silver staining with diamine or nitrate was carried out according to the methods [16][17][18][19][20]. The SYPRO Ruby staining procedure is described in Section 2.2.…”

Section: -D Electrophoresismentioning

confidence: 99%

A fluorescent natural product for ultra sensitive detection of proteins in one‐dimensional and two‐dimensional gel electrophoresis

et al. 2003

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“…The homogenate was centrifuged at 14,000 g for 15 min at 4°C and the protein concentration was estimated (Lowry et al 1951). The equal amounts (50 μg) of proteins were separated on 5 % stacking and 10 % resolving polyacrylamide slab gels (Laemmli 1970) at a constant current of 35 mA for 4 h. Separated polypeptides on the gel were visualized by the silver staining method (Switzer 1979). The gels were then scanned and photographed by gel documentation system and analyzed with quantity one software from Bio-Rad (Bio-Rad, Italy).…”

Section: Analysis Of Protein Profile By Sds-pagementioning

confidence: 99%

Copper-stress induced alterations in protein profile and antioxidant enzymes activities in the in vitro grown Withania somnifera L.

Rout

Ram

Das

et al. 2013

Physiol Mol Biol Plants

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Withania somnifera L. seedlings were grown in half-strength MS (Murashige and Skoog) basal medium for 4 weeks and then transferred to full-strength MS liquid medium for 3 weeks. The sustainable plants were subcultured in the same medium but with different concentrations (0, 25, 50, 100 and 200 μM) of Cu for 7 and 14 days. The growth parameters (root length, shoot length, leaf length and total number of leaves per plant) showed a declining trend in the treated plants in a concentration dependant manner. Roots and leaves were analyzed for protein profiling and antioxidant enzymes [catalase (CAT, EC 1.11

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“…The TCR α -chain gene was truncated to allow secretion (4,5). The cells were transfected by electroporation and plated onto RDB plates to select for incorporation of the HIS4gene.…”

Section: Antibody Screening For Secreted Proteins Expressed In Pichiamentioning

confidence: 99%

“…Samples are prepared from cell lysates, subjected to electrophoresis and stained with silver nitrate or Coomassie ® Brilliant Blue (CBB) allowing direct visualization of bands within gels (1,5). Samples can also be radiolabeled, for example with [ 35 S]methionine, and after electrophoresis, the resulting gels are dried on chromatography paper and autoradiographed (3).…”

mentioning

confidence: 99%

Use of a DNA Sequencing Gel Apparatus for Analysis of Polypeptides

Whelan

Taylor

1996

BioTechniques

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A highly sensitive silver stain for detecting proteins and peptides in polyacrylamide gels

Cited by 1,026 publications

References 8 publications

A fluorescent natural product for ultra sensitive detection of proteins in one‐dimensional and two‐dimensional gel electrophoresis

A fluorescent natural product for ultra sensitive detection of proteins in one‐dimensional and two‐dimensional gel electrophoresis

Copper-stress induced alterations in protein profile and antioxidant enzymes activities in the in vitro grown Withania somnifera L.

Use of a DNA Sequencing Gel Apparatus for Analysis of Polypeptides

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