A Highly Sensitive Fluorescence Probe for Fast Thiol‐Quantification Assay of Glutathione Reductase

Abstract

Fast detection of cellular thiols in aqueous medium was achieved using a newly developed fluorescence probe (see picture). Based on this probe, a high-throughput fluorescence assay for glutathione reductase was developed.

Styryl-BODIPY based red-emitting fluorescent OFF–ON molecular probe for specific detection of cysteine

“…Selective detection of biological thiols, such as cysteine, homocysteine and glutathione, have attracted much attention due to the pivotal role of these -SH containing molecules in physiological pathways (Chen et al, 2010a;Weh et al, 2007;Tang et al, 2007;Refsum et al, 2004;Hong et al, 2009;Lee et al, 2010;Huo et al, 2010;Shang and Dong, 2009;Yu et al, 2007;Huang et al, 2010;Yi et al, 2009;Wang et al, 2009). For example, increased white cell cysteine concentrations are only observed in individuals with cystinosis.…”

“…A wide variety of sensing mechanisms have been developed for molecular fluorescent thiol probes Chen et al, 2010a,b;Lin et al, 2010;Ruan et al, 2010;Yi et al, 2009;Amoroso et al, 2008;Lee et al, 2008;Tang et al, 2009;Zhu et al, 2010;Zhang et al, 2007Zhang et al, , 2010a. One of the methods is to attach electrophilic group to the fluorophores (e.g.…”

Oligonucleotide-stabilized fluorescent silver nanoclusters for sensitive detection of biothiols in biological fluids

“…Hcy is a risk factor for cardiovascular diseases and Alzheimer's disease (Seshadri et al, 2002). Besides traditional methods such as chromatography, mass spectroscopy, capillary electrophoresis (Jin and Wang, 1997;Lu et al, 2007;Satterfield et al, 2003), a rapidly growing interest focuses on fluorescent approaches, developing all kinds of fluorescent probes for biothiol detecting or imaging, owing to its high sensitivity, remote control and easy operation (Chen et al, 2010;Han et al, 2009;Lin et al, 2010;Liu et al, 2010;Shiu et al, 2010;Yi et al, 2009;Zhang et al, 2009). Fluorescent silver nanoclusters (Ag NCs) stabilized by singlestranded DNA or oligonucleotides (DNA-Ag NCs) in aqueous solution have attracted special attention in the recent years (Gwinn et al, 2008;O'Neill et al, 2009;Richards et al, 2008;Ritchie et al, 2007;Vosch et al, 2007;Xu and Suslick, 2010).…”

Detection of biological thiols based on a colorimetric method

“…Several methods based on chromatography, electrophoresis, and fluorescent technology for biothiol detection have been reported, especially nanomaterial-based methods much facilitated by the development of the underlying nanotechnology (Lee et al, 2008;Hong et al, 2009;Yi et al, 2009;Shiu et al, 2010;Liu et al, 2014;Zhang et al, 2014;Zhu et al, 2014). Each method has its own advantage; however, pretreatments of the materials or expensive equipments are often necessary to obtain a good performance.…”