A Highly Sensitive Fluorescence Probe for Fast Thiol‐Quantification Assay of Glutathione Reductase

Abstract

Die schnelle Detektion zellulärer Thiole in wässrigem Medium gelingt mithilfe einer neuen Fluoreszenzsonde (siehe Bild). Ein Hochdurchsatz‐Fluoreszenzassay für Glutathion‐Reduktase auf Basis dieser Sonde wurde entwickelt.

Selective fluorescent probes for live-cell monitoring of sulphide

“…The development of innovative fluorescent imaging probes has revolutionized cell biology, allowing localization and dynamic monitoring of cellular metabolite and inorganic ion pools 15,16,[24][25][26][27][28][29] . A significant bottleneck in the emerging field of H 2 S/aqueous sulphide signalling is the absence of technology for effective in vivo detection and imaging, a problem that is exacerbated by the high intracellular thiol concentration.…”

“…A major challenge is to develop molecular probes that are capable of detecting aqueous sulphides (H 2 S and HS − at neural pH) in the presence of other cellular molecules, in particular, millimolar concentrations of thiols found inside most cells. Recently, a thiolmaleimide reaction was employed to tune photoinduced electron transfer of a conjugated fluorophore for thiol detection and imaging 15 . Although effective for detection of abundant thiol molecules, this method is unable to differentiate sulphide from thiols.…”

Thioester derivatives of the natural product psammaplin A as potent histone deacetylase inhibitors

“…We first envisaged that our assay buffer could potentially be responsible for thioester hydrolysis. We prepared and used synthetic probe 10 [30] (Scheme 3) in order to test this hypothesis. Coumarin-based probe 10 is known to react extremely fast with thiols through a Michael addition reaction.…”

Chemoselective reactions and their applications in metabolite isolation and analysis.

“…Such chemoseletive (CS) reagents are usually chemically modified to suit a particular analytical technique, e.g. fluorescent detection [119,120], colorimetric assays [121][122][123], capillary electrophoresis [124], electrochemical detection [125], SALDI-MS [126] and chromatographic methods coupled with mass spectrometry(GC-MS [127], LC-MS [128]). These are designed to covalently modify (i.e., tag) glutathione for detection and quantification using UV absorption studies.…”

“…A variation of NEM where open chain Michael acceptors are used for thiol capture to induce intramolecular PET modulation of fluorescence also have been reported [49]. linked by a disulfide bond.…”