A Highly Sensitive Enzymatic Catalysis System for Trace Detection of Arsenic in Water

Liu, Yuanli; Yu, Claire; Cao, Zhixin; Shvarts, Daniel; Trogler, William C.

doi:10.1002/chem.201702769

Cited by 7 publications

(4 citation statements)

References 37 publications

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“…An aptamer (named Ars-3) claimed to bind inorganic arsenic was reported in 2009 with high affinities for both As(III) ( K d ≈ 7 nM) and As(V) ( K d ≈ 5 nM) . Arsenic is an important toxic element, , and arsenic sensors have been extensively reported, using various nanomaterials, − proteins, cells, and Raman spectroscopy. , An arsenic aptamer would expand the range of recognition molecules. Indeed, Ars-3 has become quite popular, and the number of related papers for As(III) detection has reached at least 24 to date.…”

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confidence: 99%

The Arsenic-Binding Aptamer Cannot Bind Arsenic: Critical Evaluation of Aptamer Selection and Binding

Zong

Liu

2019

Anal. Chem.

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An arsenic-binding aptamer named Ars-3 was reported in 2009, and it has been used for detection of As(III) in more than two dozen papers. In this work, we performed extensive binding assays using isothermal titration calorimetry, various DNA-staining dyes, and gold nanoparticles. By carefully comparing Ars-3 and a few random control DNA sequences, no specific binding of As(III) was observed in each case. Therefore, we conclude that Ars-3 cannot bind As(III). Possible reasons for some of the previously reported binding and detection were speculated to be related to the adsorption of As(III) onto gold surfaces, which were used in many related sensor designs, and As(III)/Au interactions were not considered before. The selection data in the original paper were then analyzed in terms of sequence alignment, secondary structure prediction, and dissociation constant measurement. These steps need rigorous testing before confirming specific binding of newly selected aptamers. This study calls for attention to the gap between aptamer selection and biosensor design, and the gap needs to be filled by careful binding assays to further the growth of the aptamer field.

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confidence: 99%

The Arsenic-Binding Aptamer Cannot Bind Arsenic: Critical Evaluation of Aptamer Selection and Binding

Zong

Liu

2019

Anal. Chem.

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“…But mo s t are toxic, difficult to separate after adsorption, and ineffective in impurities. Therefore, iron oxide and its modifications overcome these limitations [89][90][91][92][93]. Liu and co-workers [93] proposed an arsenate detection method that relies on the s trong interaction between As (V) and the surface of metal oxides.…”

Section: Fluorometric Determination Of Arsenate 231detection Based On...mentioning

confidence: 99%

“…Therefore, iron oxide and its modifications overcome these limitations [89][90][91][92][93]. Liu and co-workers [93] proposed an arsenate detection method that relies on the s trong interaction between As (V) and the surface of metal oxides. They synthesized fluorescent DNA-loaded ferric oxide nanoparticles by incubating carboxyfluorescein (FAM)labeled DNA with nanoparticles.…”

Section: Fluorometric Determination Of Arsenate 231detection Based On...mentioning

confidence: 99%

Colorimetric and Fluorometric detection of arsenic: arsenate and arsenite

Nawarathne

Weerasinghe

Dharmarathne³

2023

Anal. Method Environ. Chem. J.

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Arsenic is a highly toxic metalloid that forms different chemical states in nature, including arsenate and arsenite, as common inorganic forms. Exposure to arsenic may cause adverse effects on human health and the environment. Therefore, the detection of arsenic is critical. Exploring new approaches with low detection ranges and high sensitivity is crucial. This review paper consists of optical methods, including colorimetric and fluorometric methods, which detect arsenite and arsenate. Initially proposed colorimetric approaches such as the Gutzeit and molybdenum blue method can easily to use. However, the production of toxic substances limits their applications. Later, structurally modified molecules, nanoparticle-based assays, and their modifications are used for arsenic detection. Fluorometric methods also have noticeable attention to arsenic detection. Fluorescent approaches reported in this paper are based on semiconductor nanomaterials, other nanomaterials, and their modifications, etc. In addition, arsenate's catalytic and inhibitory activity on enzyme activity can be used to detect arsenic through colorimetric and fluorometric methods. This review highlighted the advantages, disadvantages, comparisons, and uses of colorimetric and fluorometric methods in detecting arsenite and arsenate.

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“…The devices that are in use for on-line monitoring can be divided in three categories. The first are sensors that measure chemical or physical parameters, such as temperature, conductivity, turbidity, acidity, metal ions [ 6 , 7 , 8 ], or oxygen content. The second group measures several specific organic pollutants by chemical analysis such as on-line HPLC-UV.…”

Section: Introductionmentioning

confidence: 99%

Development and Validation of an On-Line Water Toxicity Sensor with Immobilized Luminescent Bacteria for On-Line Surface Water Monitoring

Woutersen

Gaag²,

Boakye

et al. 2017

Sensors

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Surface water used for drinking water production is frequently monitored in The Netherlands using whole organism biomonitors, with for example Daphnia magna or Dreissena mussels, which respond to changes in the water quality. However, not all human-relevant toxic compounds can be detected by these biomonitors. Therefore, a new on-line biosensor has been developed, containing immobilized genetically modified bacteria, which respond to genotoxicity in the water by emitting luminescence. The performance of this sensor was tested under laboratory conditions, as well as under field conditions at a monitoring station along the river Meuse in The Netherlands. The sensor was robust and easy to clean, with inert materials, temperature control and nutrient feed for the reporter organisms. The bacteria were immobilized in sol-gel on either an optical fiber or a glass slide and then continuously exposed to water. Since the glass slide was more sensitive and robust, only this setup was used in the field. The sensor responded to spikes of genotoxic compounds in the water with a minimal detectable concentration of 0.01 mg/L mitomycin C in the laboratory and 0.1 mg/L mitomycin C in the field. With further optimization, which should include a reduction in daily maintenance, the sensor has the potential to become a useful addition to the currently available biomonitors.

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A Highly Sensitive Enzymatic Catalysis System for Trace Detection of Arsenic in Water

Cited by 7 publications

References 37 publications

The Arsenic-Binding Aptamer Cannot Bind Arsenic: Critical Evaluation of Aptamer Selection and Binding

The Arsenic-Binding Aptamer Cannot Bind Arsenic: Critical Evaluation of Aptamer Selection and Binding

Colorimetric and Fluorometric detection of arsenic: arsenate and arsenite

Development and Validation of an On-Line Water Toxicity Sensor with Immobilized Luminescent Bacteria for On-Line Surface Water Monitoring

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