A highly sensitive assay of human plasma xanthine oxidoreductase activity using stable isotope-labeled xanthine and LC/TQMS

Murase, Takayo; Nampei, Mai; Oka, Mitsuru; Miyachi, Atsushi; Nakamura, Takashi

doi:10.1016/j.jchromb.2016.10.033

Cited by 76 publications

(95 citation statements)

References 24 publications

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“…Another study used a more recent and highly sensitive assay of xanthine oxidoreductase activity to demonstrate the accelerated conversion of xanthine dehydrogenase (XDH) to xanthine oxidase (XO) with renal dysfunction. 50 The results of these two studies are in agreement with the conclusion of this meta-analysis. One study in the literature demonstrated that xanthine oxidoreductase (XOR) activity was lower among patients with renal dysfunction.…”

Section: Discussionsupporting

confidence: 88%

Allopurinol and endothelial function: A systematic review with meta‐analysis of randomized controlled trials

Alem

2018

Cardiovascular Therapeutics

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SummaryAimOxidative stress and endothelial dysfunction are two inter‐related conditions commonly seen in patients with cardiovascular risk factors. The enzyme, xanthine oxidase, is an important contributor to these phenomena but to a variable degree in different patient populations. This meta‐analysis will summarize the effect of allopurinol, an established xanthine oxidase inhibitor, on endothelial function among patients with different comorbidities.MethodsMedline Complete, PubMed, ProQuest, ClinicalKey, Wiley Online Library, and Cochrane Central Register of Controlled Trials were searched till July 29, 2017. Meta‐analysis was planned for randomized controlled trials (RCTs) that investigated allopurinol effects on endothelial function. A random effect model was used to calculate the standardized mean difference (with 95% confidence intervals: CI) as an estimate of effect size. Heterogeneity was quantified by four types of information: Q statistics, I 2 statistic, Tau‐squared (T 2), and Tau (T).ResultsThirty eligible studies were identified; 12 were included in the final analysis and subdivided among 3 patient’s groups: patients with chronic heart failure (CHF; 197 patients), patients with chronic kidney disease (CKD; 183 patients), and patients with type 2 diabetes mellitus (DM; 170 patients). Allopurinol was found to have a statistically significant benefit on endothelial function in patients with CHF and CKD but not in type 2 DM. The standardized mean differences and CI in the three patient’s groups were 0.776 (0.429, 1.122), 0.350 (0.009, 0.690), and 1.331 (−0.781, 3.444), respectively.ConclusionAllopurinol has an antioxidant property that might partially reverse endothelial dysfunction in patients with certain comorbidities. The importance of this property and the magnitude of the beneficial effect are likely to be related to the relative contribution of xanthine oxidase into the oxidative stress associated with different underlying pathologies.

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Section: Discussionsupporting

confidence: 88%

Allopurinol and endothelial function: A systematic review with meta‐analysis of randomized controlled trials

Alem

2018

Cardiovascular Therapeutics

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“…However, such a method resulted in relatively low sensitivities. Even in recent reports, considerable numbers of plasma samples were below the detectable range in assays of XO activity. In other assays, the production of ROS was measured for assessing plasma XO activity.…”

Section: Discussionmentioning

confidence: 99%

Activity of xanthine oxidase in plasma correlates with indices of insulin resistance and liver dysfunction in patients with type 2 diabetes mellitus and metabolic syndrome: A pilot exploratory study

Sunagawa

Saito

Hokama

et al. 2018

J of Diabetes Invest

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“…Measurement of XOR and XO activities was performed by the method previously reported by Murase et al using liquid chromatography combined with triple quadrupole mass spectrometry (LC-TQMS method) [15]. Briefly, specimens were purified by Sephadex G25 column and were added to Tris buffer (pH 8.5) containing [ 13 C 2 , 15 N 2 ]xanthine, NAD+, and [ 13 C 3 , 15 N 3 ]uric acid.…”

Section: Methodsmentioning

confidence: 99%

The Relationship between Xanthine Oxidoreductase and Xanthine Oxidase Activities in Plasma and Kidney Dysfunction

Terawaki¹,

Murase²,

Nakajima³

et al. 2017

J Clin Exp Nephrol

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Background: Oxidative stress (OS) is thought to play a role in detrimental events among patients with chronic kidney disease (CKD). Although the mechanism of OS increases in CKD patients is unclear, it has been suggested that increased activity of xanthine oxidase (XO), the superoxideproducing form of xanthine oxidoreductase (XOR), plays a large role in enhancing OS. Therefore, we measured the activities of plasma XOR and XO among CKD patients.

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A highly sensitive assay of human plasma xanthine oxidoreductase activity using stable isotope-labeled xanthine and LC/TQMS

Cited by 76 publications

References 24 publications

Allopurinol and endothelial function: A systematic review with meta‐analysis of randomized controlled trials

Allopurinol and endothelial function: A systematic review with meta‐analysis of randomized controlled trials

Activity of xanthine oxidase in plasma correlates with indices of insulin resistance and liver dysfunction in patients with type 2 diabetes mellitus and metabolic syndrome: A pilot exploratory study

The Relationship between Xanthine Oxidoreductase and Xanthine Oxidase Activities in Plasma and Kidney Dysfunction

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