A highly sensitive and efficient UPLC‐MS/MS assay for rapid analysis of tedizolid (a novel oxazolidinone antibiotic) in plasma sample

Abstract

Tedizolid (TDZ) is a novel oxazolidinone class antibiotic, indicated for the treatment of acute bacterial skin and skin structure infections in adults. In this study a highly sensitive UPLC-MS/MS assay was developed and validated for the determination of TDZ in rat plasma using rivaroxaban as an internal standard (IS). Both TDZ and IS were separated on an Acquity UPLC BEH™ C column using an isocratic mobile phase comprising of acetonitrile-20 mm ammonium acetate (85:15, v/v), eluted at 0.3 mL/min flow rate. The plasma sample was processed by liquid liquid extraction technique using ethyl acetate as an extracting agent. The analyte and IS were detected in positive mode using electrospray ionization source. The precursor to product ion transitions at m/z 371.09 > 343.10 for TDZ and m/z 435.97 > 144.94 for IS were used for the quantification in multiple reaction monitoring mode. The calibration curve was linear in the concentration range of 0.74-1500 ng/mL and the lower limit of quantification was 0.74 ng/mL only. The developed assay was validated following standard guidelines for bioanalytical method validation (US Food and Drug Administration) and all the validation results were within the acceptable limits. The developed assay was successfully applied into a pharmacokinetic study in rats. Copyright © 2016 John Wiley & Sons, Ltd.

UHPLC–MS/MS method for simultaneous quantification of doripenem, meropenem, ciprofloxacin, levofloxacin, pazufloxacin, linezolid, and tedizolid in filtrate during continuous renal replacement therapy

“…26 Meanwhile, the ranges of calibration curves for measuring plasma concentrations using other previously reported methods were 0.05-100 μg/ml for DRPM, 20 0.1-100 μg/ml for MEPM, 23 0.025-30 μg/ml for CPFX, 19 0.09-4.53 μg/ml for LVFX, 25 0.02-0.5 μg/ml for PZFX, 22 1-100 μg/ ml for LZD, 24 and 0.00074-1.5 μg/ml for TZD. 21 Comparing our results with other reports, the concentration ranges of our calibration curves are relatively broad. Our pretreatment method is more straightforward and can extract multiple drugs in the same batch, whereas more complicated methods such as solid-phase extraction and ethanol precipitation extraction were used in other studies.…”

“…Our pretreatment method is more straightforward and can extract multiple drugs in the same batch, whereas more complicated methods such as solid‐phase extraction and ethanol precipitation extraction were used in other studies 19–25 . The run time of our method is also shorter (7 min 26 ) than previous reports (8–22 min) 19–25 . Our UHPLC–MS/MS assay yields sharper peaks 26 compared with some methods that used high‐performance liquid chromatography (HPLC) with ultraviolet (UV) detection 20,22,23 …”

“…The concentrations and solvents used to prepare stock solutions of the antibiotics were determined by referring to previous reports. [19][20][21][22][23][24][25] Polypropylene tubes were used in this study, because our previous study found no adsorption of any drug to polypropylene tubes. 26 The stock solutions of standards and IS were prepared at the following concentrations using the solvent indicated: DRPM (1 mg/ml in 25% MeOH), DPRM-d 4 (0.1 mg/ml in 25% MeOH), MEPM (1 mg/ml in 25% MeOH), MEPM-d 6 (0.1 mg/ml in 25% MeOH), LZD (2.5 mg/ml in 25% MeOH), LZD-d 3 (0.1 mg/ml in 25% MeOH), TZD (0.2 mg/ml in dimethyl sulfoxide), TZD-d 3 (0.04 mg/ml in MeOH), CPFX (1 mg/ ml in 25% MeOH + 20 mM acetic acid), CPFX-d 8 (0.25 mg/ml in 50% MeOH + 20 mM acetic acid), LVFX (1 mg/ml in 20% MeOH), LVFX-d 8 (0.1 mg/ml in 20% MeOH), PZFX [1 mg/ml in MeOH + HCl (99.5:0.5, v/v)], and PZFX-d 4 [0.1 mg/ml in MeOH + HCl (99.5:0.5, v/v)].…”

Perspectives of Positively Charged Nanocrystals of Tedizolid Phosphate as a Topical Ocular Application in Rabbits

“…The “Multiple reactions monitoring (MRM) was used for the quantification of TDZ and IS with the parent to daughter ion transitions ( m / z ) of 371.15→343.17 and 338.18→296.22, respectively”. “The UPLC-MS/MS system was operated by Mass-Lynx Software (V-4.1, SCN-714)” while the obtained chromatograms were processed by the “Target Lynx TM program” as reported [ 36 , 49 ].…”

Oxazolidinone Antibiotics: Chemical, Biological and Analytical Aspects

“…HPLC-MS/MS was also used [ 38 , 39 ] for the simultaneous quantitation of OTB-658 (a novel oxazolidinone anti-tuberculosis agent) and its two major metabolites in monkey blood with good linearity over the range of 10–2000 ng/mL. Very recently, UPLC-MS/MS methods were reported for the determination of TDZ in plasma [ 40 ] and in aqueous humor of rabbit, with a LOD of 1.97 ng/mL [ 38 , 39 ].…”