A highly sensitive and colorimetric biosensor based on magnetic nano‐DNAzyme for detection of lead (II) ion in real water samples

Rong, Mingge; Li, Jun; Hu, Jiahui; Chen, Aolong; Wu, Weibing; Lyu, Jianxin

doi:10.1002/jctb.5684

Cited by 21 publications

(3 citation statements)

References 50 publications

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“…7 Nanozymes, which have the advantages of convenience in preparation 8 and extraordinary stability in harsh environments, have been studied as probes to improve the sensitivity due to their high catalytic efficiency. As representative nanozymes, metal, 9 metal oxide, 10 and carbon-based nanomaterials 11 have been gradually employed in clinical, 12 food safety, 13 agricultural, 14 and environmental 15 fields. In particular, bimetallic catalytic NPs, such as Pt/Pd, 16 have been used as promising materials for signal amplification in ICA.…”

Section: ■ Introductionmentioning

confidence: 99%

Synthesis of PDA-Mediated Magnetic Bimetallic Nanozyme and Its Application in Immunochromatographic Assay

Lai

Zhang

Zeng

et al. 2020

ACS Appl. Mater. Interfaces

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Immunochromatographic assay (ICA) is widely applied in various fields. However, severe matrix interference and weak signal output present major challenges in achieving accurate and ultrasensitive detection in ICA. Here, a polydopamine (PDA)-mediated magnetic bimetallic nanozyme (Fe 3 O 4 @PDA@Pd/Pt) with peroxidase-like activity was synthesized and used as a probe in ICA. The magnetic property of Fe 3 O 4 @PDA@Pd/Pt enabled effective magnetic enrichment of targets, thereby reducing the matrix interference in the sample. PDA coating on the magnetic bimetallic nanozyme was employed as a mediator and a stabilizer. It improved the catalytic ability and stability of the magnetic bimetallic nanozyme by providing more coordination sites for Pd/Pt growth and functional groups (−NH and −OH). In addition, the Pd/Pt bimetallic synergistic effect could further enhance the catalytic ability of the nanozyme. A method was developed by integrating Fe 3 O 4 , PDA, and Pd/Pt into Fe 3 O 4 @PDA@Pd/Pt as a probe in ICA. With the proposed method, human chorionic gonadotropin and Escherichia coli O157:H7 were successfully detected to be as low as 0.0094 mIU/mL in human blood serum and 9 × 10 1 CFU/mL in the milk sample, respectively. This method may be readily adapted for accurate and ultrasensitive detection of other biomolecules in various fields.

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Section: ■ Introductionmentioning

confidence: 99%

Synthesis of PDA-Mediated Magnetic Bimetallic Nanozyme and Its Application in Immunochromatographic Assay

Lai

Zhang

Zeng

et al. 2020

ACS Appl. Mater. Interfaces

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show abstract

“…Temperature control, such as electrically heated indium-tin-oxide electrode, can help to adjust and keep an optimum temperature for such biosensors (Ni et al, 2019). The pH values can control the cleavage of RNA linkage, pH variance can sensitively impact the stability and sensitivity of the DNAzyme biosensors (Chen et al, 2016, Cai et al, 2017, Liu et al, 2017c, Rong et al, 2018.…”

Section: Dnazymesmentioning

confidence: 99%

Progress of optical biosensors for analyzing pathogens and organic pollutants in water since 2015

et al. 2022

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Water contamination by pathogens and organic pollutants is one of the major environmental problems that risk human health. Climate change with extreme weather can promote their prevalence in waters. Environmental monitoring of these pollutants in a fast, continuous, and accurate manner is of increasing demand, especially under the climate change context, but is challenged by their ubiquity and trace concentrations. Optical biosensing is one of the desired solutions owing to its rapid and accurate detection with high sensitivity. Principally, an optical biosensor recognizes these bioactive toxins and contaminants by tailored bioreceptors (e.g., aptamer, enzyme, and cells) and transduces the biological response to optical signals. Research efforts have been made on tailoring bioreceptors and enhancing signal transducing by nanoparticles. This study comprehensively reviewed the mechanisms of optical biosensing and the recent development of bioreceptors and nanomaterials on the enhancement for the rapid, easy, and accurate analysis of emerging contaminants in water. The advantages and challenges on sensitivity, selectivity, and durability of biosensors were discussed along with the opportunities and development strategies.

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“…A similar design was also studied using GR5 DNAzyme for Pb 2+ detection. [ 118 ] In both cases, HRP catalyzed the oxidation of TMB to produce blue color for the detection.…”

Section: Dnazymesmentioning

confidence: 99%

Interfacing Catalytic DNA with Nanomaterials

Moon

Liu

2020

Adv Materials Inter

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DNAzymes are catalytic DNA strands with interesting features such as high stability, versatile activity, and programmability. Interfacing DNAzymes with nanomaterials has boosted their function to achieve biosensing, intracellular imaging, smart materials, cleavage of viral/cancer RNA, and enhancing substrate specificity. This review starts with the introduction of a few commonly used DNAzymes for RNA cleavage, DNA cleavage, and peroxidation. The interactions of DNA and DNAzymes with various inorganic surfaces including gold, metal oxides, carbon‐based nanomaterials, metal–organic frameworks, and hydrogels are then discussed. DNAzymes can be adsorbed, covalently linked, or entrapped in these nanomaterials. After that, representative examples of applications are reviewed with an emphasis on the DNA/nanomaterials’ interfaces and fundamental chemical interactions. These examples include using nanomaterials for adsorbing DNAzymes and fluorescence quenching, producing a color change, assisting DNAzymes entering cells, supplying extra metal ions, and for molecularly imprinting target molecules. Finally, some key challenges in the field are discussed, and future research opportunities addressing these challenges are proposed.

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A highly sensitive and colorimetric biosensor based on magnetic nano‐DNAzyme for detection of lead (II) ion in real water samples

Cited by 21 publications

References 50 publications

Synthesis of PDA-Mediated Magnetic Bimetallic Nanozyme and Its Application in Immunochromatographic Assay

Synthesis of PDA-Mediated Magnetic Bimetallic Nanozyme and Its Application in Immunochromatographic Assay

Progress of optical biosensors for analyzing pathogens and organic pollutants in water since 2015

Interfacing Catalytic DNA with Nanomaterials

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