A Highly Selective Fluorescence Turn‐On Sensor for Extracellular Calcium Ion Detection

Sui, Binglin; Liu, Xinglei; Wang, Mengyuan; Belfield, Kevin D.

doi:10.1002/chem.201602162

Cited by 30 publications

(27 citation statements)

References 24 publications

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“…After that, 20 μL of the CellTiter 96 Aqueous One solution reagent (for MTS assay) was added into each well, followed by further incubation for 2 h at 37 °C. 17 , 31 , 36 The respective absorbance values were read on a Tecan Infinite M200 PRO plate reader spectrometer at 490 nm to determine the relative amount of formazan produced. Cell viabilities were calculated on the basis of the following equation (eq 3) where Abs s 490nm is the absorbance of the cells incubated with different concentrations of experimental probe solutions, Abs D 490nm is the absorbance of cell-free well containing only dye at the concentration that was studied, Abs c 490nm is the absorbance of cells only incubated in the medium, Abs D2 490nm is the absorbance of the cell-free well.…”

Section: Methodsmentioning

confidence: 99%

Fluorenyl-Loaded Quatsome Nanostructured Fluorescent Probes

et al. 2017

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Delivery of hydrophobic materials in biological systems, for example, contrast agents or drugs, is an obdurate challenge, severely restricting the use of materials with otherwise advantageous properties. The synthesis and characterization of a highly stable and water-soluble nanovesicle, referred to as a quatsome (QS, vesicle prepared from cholesterol and amphiphilic quaternary amines), that allowed the nanostructuration of a nonwater soluble fluorene-based probe are reported. Photophysical properties of fluorenyl–quatsome nanovesicles were investigated via ultraviolet–visible absorption and fluorescence spectroscopy in various solvents. Colloidal stability and morphology of the nanostructured fluorescent probes were studied via cryogenic transmission electronic microscopy, revealing a “patchy” quatsome vascular morphology. As an example of the utility of these fluorescent nanoprobes, examination of cellular distribution was evaluated in HCT 116 (an epithelial colorectal carcinoma cell line) and COS-7 (an African green monkey kidney cell line) cell lines, demonstrating the selective localization of C-QS and M-QS vesicles in lysosomes with high Pearson’s colocalization coefficient, where C-QS and M-QS refer to quatsomes prepared with hexadecyltrimethylammonium bromide or tetradecyldimethylbenzylammonium chloride, respectively. Further experiments demonstrated their use in time-dependent lysosomal tracking.

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Section: Methodsmentioning

confidence: 99%

Fluorenyl-Loaded Quatsome Nanostructured Fluorescent Probes

et al. 2017

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“…Fig. 8 shows some of the imine linkagebased COFs (13)(14)(15)(16)(17)(18) that have been realized by the condensation of amine and aldehyde building blocks. [49][50][51][52][53][54][55] The photoluminescence properties of these COFs have not been investigated, although some of these frameworks contain 1,3,5-TPB units.…”

Section: Discrete 135-tris(4 0 -Aminophenyl)benzenesmentioning

confidence: 99%

“…Another development in the area of chemo-sensing is the case of uorescence turn-on sensors. 17,18 Typically, uorescence turn-on sensors consist of uorophore and guest-receptor units, connected to each other by a covalent linkage. The pristine sensors exhibit very weak uorescence due to intramolecular PET ( Fig.…”

Section: Introductionmentioning

confidence: 99%

1,3,5-Triphenylbenzene: a versatile photoluminescent chemo-sensor platform and supramolecular building block

2018

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“…Calcium (Ca 2+ ) is one of the most abundant metals in the human body, making up to 2%wt of total human body weight, and plays a fundamental role in biological processes including secondary messengers critical for cell signalling, protein folding, and catalysis [ 1 , 2 , 3 , 4 ]. It has also been demonstrated that monitoring Ca 2+ concentrations is important within the environment.…”

Section: Introductionmentioning

confidence: 99%

DNAzyme Sensor for the Detection of Ca2+ Using Resistive Pulse Sensing

Heaton

Platt

2020

Sensors

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DNAzymes are DNA oligonucleotides that can undergo a specific chemical reaction in the presence of a cofactor. Ribonucleases are a specific form of DNAzymes where a tertiary structure undergoes cleavage at a single ribonuclease site. The cleavage is highly specificity to co-factors, which makes them excellent sensor recognition elements. Monitoring the change in structure upon cleavage has given rise to many sensing strategies; here we present a simple and rapid method of following the reaction using resistive pulse sensors, RPS. To demonstrate this methodology, we present a sensor for Ca2+ ions in solution. A nanoparticle was functionalised with a Ca2+ DNAzyme, and it was possible to follow the cleavage and rearrangement of the DNA as the particles translocate the RPS. The binding of Ca2+ caused a conformation change in the DNAzyme, which was monitored as a change in translocation speed. A 30 min assay produced a linear response for Ca2+ between 1–9 μm, and extending the incubation time to 60 min allowed for a concentration as low as 0.3 μm. We demonstrate that the signal is specific to Ca2+ in the presence of other metal ions, and we can quantify Ca2+ in tap and pond water samples.

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A Highly Selective Fluorescence Turn‐On Sensor for Extracellular Calcium Ion Detection

Cited by 30 publications

References 24 publications

Fluorenyl-Loaded Quatsome Nanostructured Fluorescent Probes

Fluorenyl-Loaded Quatsome Nanostructured Fluorescent Probes

1,3,5-Triphenylbenzene: a versatile photoluminescent chemo-sensor platform and supramolecular building block

DNAzyme Sensor for the Detection of Ca2+ Using Resistive Pulse Sensing

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