A highly selective and sensitive nanoprobe for detection and imaging of the superoxide anion radical in living cells

Li, Na; Wang, Hui; Xue, Ming; Chang, Chen; Chen, Zhenzhen; Zhuo, Linhai; Tang, Bo

doi:10.1039/c2cc16376d

Cited by 43 publications

(21 citation statements)

References 22 publications

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“…Our group designed and synthesized a novel DBZTC/Ag@SiO 2 hybrid fluorescent nanoprobe 18 with high sensitivity and selectivity for detection and imaging of O 2 .− in living cells . Probe 18 was constructed through a simple self‐assembly method based on the previous probe 2 and Ag@SiO2 core–shell nanoparticles (Figure A).…”

Section: Current Status Of Fluorescent Probes For O2−mentioning

confidence: 99%

Versatile Fluorescent Probes for Imaging the Superoxide Anion in Living Cells and In Vivo

et al. 2019

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The superoxide anion (O2.−) is widely engaged in the regulation of cell functions and is thereby intimately associated with the onset and progression of many diseases. To ascertain the pathological roles of O2.− in related diseases, developing effective methods for monitoring O2.− in biological systems is essential. Fluorescence imaging is a powerful tool for monitoring bioactive molecules in cells and in vivo owing to its high sensitivity and high temporal‐spatial resolution. Therefore, increasing numbers of fluorescent imaging probes have been constructed to monitor O2.− inside live cells and small animals. In this minireview, we summarize the methods for design and application of O2.−‐responsive fluorescent probes. Moreover, we present the challenges for detecting O2.− and suggestions for constructing new fluorescent probes that can indicate the production sites and concentration changes in O2.− as well as O2.−‐associated active molecules in living cells and in vivo.

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Section: Current Status Of Fluorescent Probes For O2−mentioning

confidence: 99%

Versatile Fluorescent Probes for Imaging the Superoxide Anion in Living Cells and In Vivo

et al. 2019

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“…12 where optimal fluorescence enhancement with a maximum enhancement factor of 3.1 and 1.82 was obtained with silica shell thickness of 20 nm and 50 nm, respectively. Ag@SiO 2 was treated with aminosilane compound and the DBZTC was loaded onto Ag@SiO 2 surface through reaction between -NH 2 and Cl − functional group.…”

mentioning

confidence: 99%

The Distance-Dependent Fluorescence Enhancement Phenomena in Uniform Size Ag@SiO₂@SiO₂(dye) Nanocomposites

Arifin¹,

Lee²

2013

Bulletin of the Korean Chemical Society

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Ag@SiO 2 @SiO 2 (FITC) nanocomposites were prepared by the simple polyol process and Stöber method. Fluorescence enhancement of fluorescein moiety (fluorescein isothiocyanate, FITC) was investigated in the presence of silver nanoparticles in Ag@SiO 2 -X@SiO 2 (FITC) system with varying thickness (X nm) of first silica shell. Maximum enhancement factor of 4.3 fold was achieved in Ag@SiO 2 -8@SiO 2 (FITC) structure with the first silica shell thickness of 8 nm and the average separation distance of 11 nm between the surface of silver nanoparticle and fluorescein moiety. The enhancement is believed to be originated from increased excitation rate of fluorescein moiety due to concentrated local electromagnetic field which was improved by interaction of light with silver nanoparticles.

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“…Overaccumulation of ROS can induce oxidative stress, which is related to many diseases such as diabetes, cancer and molecular neurodegenerative disorders [2,10,24]. Several techniques/assays have been used to determinate ROS including electron spin resonance (ESR) spectroscopy, fluorescence spectroscopy, chemiluminescence assay and electrochemical biosensors [1,16,17,28,3]. Among of these methods, fluorescence-based assays can be used for real-time monitoring ROS level in cells and tissues [4,11,9].…”

Section: Introductionmentioning

confidence: 99%

Cy5 labeled single-stranded DNA-polydopamine nanoparticle conjugate-based FRET assay for reactive oxygen species detection

Lei

Liu

2015

Sensing and Bio-Sensing Research

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a b s t r a c tThis work reports on a simple and feasible fluorescence resonance energy transfer (FRET) assay for detecting reactive oxygen species (ROS) both in solution and living cell using polydopamine nanoparticle (PDA NP) as energy acceptor and Cy5 labeled single-stranded DNA (Cy5-ssDNA) as energy donor. The Cy5-ssDNA and PDA NPs form self-assembled conjugates (Cy5-ssDNA-PDA NP conjugates) via p-stacking interactions. In the presence of ROS, the PDA NP adsorbed Cy5-ssDNAs can be effectively cleaved, resulting in the release of Cy5 molecules into solution and recovery of fluorescence emission of Cy5. In order to obtain ROS solution, the glucose oxidase-catalyzed oxidation reaction of glucose with O 2 is employed to generate hydrogen peroxide for Fenton-like reaction. The formation of ROS in Fenton-like reaction can be detected as low as glucose oxidase-catalyzed oxidation of 100 pM glucose by the Cy5-ssDNA-PDA NP conjugate-based FRET assay. The recovery ratio of Cy5 fluorescence intensity is increased linearly with logarithm of glucose concentration from 100 pM to 1 lM, demonstrating that the FRET assay has wide dynamic range. In particular, intracellular ROS has been successfully detected in chemical stimulated HepG-2 cells by the Cy5-ssDNA-PDA NP conjugate-based FRET assay with a fluorescence microscopy, indicating that this approach has great potential to monitor ROS in living cells.

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A highly selective and sensitive nanoprobe for detection and imaging of the superoxide anion radical in living cells

Cited by 43 publications

References 22 publications

Versatile Fluorescent Probes for Imaging the Superoxide Anion in Living Cells and In Vivo

Versatile Fluorescent Probes for Imaging the Superoxide Anion in Living Cells and In Vivo

The Distance-Dependent Fluorescence Enhancement Phenomena in Uniform Size Ag@SiO₂@SiO₂(dye) Nanocomposites

Cy5 labeled single-stranded DNA-polydopamine nanoparticle conjugate-based FRET assay for reactive oxygen species detection

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A highly selective and sensitive nanoprobe for detection and imaging of the superoxide anion radical in living cells

Cited by 43 publications

References 22 publications

Versatile Fluorescent Probes for Imaging the Superoxide Anion in Living Cells and In Vivo

Versatile Fluorescent Probes for Imaging the Superoxide Anion in Living Cells and In Vivo

The Distance-Dependent Fluorescence Enhancement Phenomena in Uniform Size Ag@SiO2@SiO2(dye) Nanocomposites

Cy5 labeled single-stranded DNA-polydopamine nanoparticle conjugate-based FRET assay for reactive oxygen species detection

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The Distance-Dependent Fluorescence Enhancement Phenomena in Uniform Size Ag@SiO₂@SiO₂(dye) Nanocomposites