A Highly Predictable Animal Model of Retinoblastoma

Kobayashi, Masato; Mukai, Noritsugu; Solish, Samuel P.; Pomeroy, M.

doi:10.1097/00005072-198205000-00092

Cited by 3 publications

(5 citation statements)

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“…However, centrosomes were present. In contrast to the original descriptions (Kobayashi et al 1982), neurosecretory granules and beta-glycogen particles were noticed in several cells.…”

Section: Discussioncontrasting

confidence: 95%

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Characterization of an intraocular retinoblastoma‐like tumour

Winther

Jensen

Prause

et al. 1987

Acta Ophthalmologica

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A transplantable intraocular retinoblastoma-like tumour growing in F 344 rat eyes and tissue cultures suitable for therapeutic experiments is characterized. The tumours grew in the vitreous and infiltrated the internal layers of the retina. Feeder vessels from the retina supplied the tumours. Few and often incomplete rosettes were seen, and calcification was sparse in areas of necrosis. Stainings for glial fibrillary acidic protein, S-100 protein, neuron-specific enolase and desmin were negative. Ultrastructural analysis brought out a close correspondence between the tumour cell and the human retinoblastoma and other virus-induced retinoblastoma-like tumours. The experimental tumour cells lacked cilia, but had neurosecretory granules. Chromosome analysis showed a modal chromosome number in the triploid range, and 6 marker chromosomes were demonstrated. Flow-cytometric analysis showed a S-phase cell fraction of 42%, and chromosome stability was suggested by a tumour-cell DNA index which remained stable for more than 3 months.

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“…However, centrosomes were present. In contrast to the original descriptions (Kobayashi et al 1982), neurosecretory granules and beta-glycogen particles were noticed in several cells.…”

Section: Discussioncontrasting

confidence: 95%

“…In addition, necroses and inflammatory reactions were more pronounced, and calcifications were scarce. Therefore, light microscopically the tumour was fully comparable with the tumour described by Kobayashi et al (1982), except that the observed rosettes had no resemblance to Homer-Wright rosettes, as described originally.…”

Section: Discussionsupporting

confidence: 73%

Characterization of an intraocular retinoblastoma‐like tumour

Winther

Jensen

Prause

et al. 1987

Acta Ophthalmologica

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“…The retinoblastoma-like tumour cells were kindly supplied by Dr N. Mukai, Eye Research Institute of Retina Foundation, Boston, MA. The cell line has been established from a human adenovirus-12-induced retina tumour in F 344 rats (Kobayashi et al 1982).…”

Section: Tumour Cellsmentioning

confidence: 99%

“…Only three transplantable intraocular retinoblastoma-like models have been described to date (Rosemaria et al 1977;Gallie et al 1977;Kobayashi et al 1982), and experimental therapeutical studies have only rarely been reported (Benedict et al 1980b;Kobayashi et al 1983a;Totsaku & Minoda 1982;Schipper et al 1984).…”

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confidence: 99%

In vitro and in vivo growth of an intraocular retinoblastoma‐like tumour in F‐344 rats

Winther

1986

Acta Ophthalmologica

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Retinoblastoma-like cells grew in colonies on the bottom of tissue culture flasks. The population doubling time was 19 h. Tumour cells from cell cultures had a 39% plating efficiency, and fresh tumour cells from intraocular tumours had a 32% plating efficiency in colony forming assays. Inoculation of 1.5 X 10(4) tumour cells in the vitreous of F-344 rats resulted in a 100% tumour take and regularly growing tumours with a doubling time of 3 days. The tumour take-rate was not changed in wholebody immunosuppressed animals. The tumour volume was assessed under a stereo microscope, and it was possible to divide the tumours into 4 groups according to the number of intraocular tumour cells. Tumour growth caused eye perforation in 89% of the inoculated eyes. Spontaneous tumour regression was not seen in non-perforation groups. Immunosuppression with whole-body irradiation and dense traumatic cataract had no significant effect on the growth. It is concluded that this animal retinoblastoma-like tumour is suitable for quantitative therapy studies in vivo and in vitro.

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“…DNA was extracted from the eyes of Ad12 inoculated rats (3, 9,12,24,48 or 72 h after virus inoculation). When the Ad12 oncogene H A fragment was used as a probe, hybridized bands were observed in each of the cases (Fig.…”

Section: Southern Blot Analyslsmentioning

confidence: 99%

Early stage of human adenovirus type 12‐inoculated retinal tissue of F344 newborn rat

Kato

Yamamoto

Sawada

et al. 1996

Pathology International

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To elucidate the pathogenesis of adenovirus type 12 (Ad12)-induced rat retinal tumor, an experimental animal model of human retinoblastoma (RB), DNA analysis, in situ hybridization and immunohistochemistry were performed. The adenovirus oncogene E1A was detected in the host genome by Southern blot hybridization. Examined retinal tissues did not show any histological changes, but the number of retinal cells immunoreactive with an antibody to proliferating cell nuclear antigen (PCNA) increased with the course of study. In in situ hybridization, E1A gene expression was recognized at the inner granular layer of the retina at an early stage after virus inoculation, and subsequently, N-myc gene expression was recognized at the same region. No alteration was found in the retinoblastoma susceptibility gene (Rb gene) expression. The product of the virus oncogene integrated into the host genome could induce an increase in N-myc expression, without any abnormality of the Rb gene itself. Results from the present study could be useful in clarifying the tumorigenesis of this experimental model.

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A Highly Predictable Animal Model of Retinoblastoma

Cited by 3 publications

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Characterization of an intraocular retinoblastoma‐like tumour

Characterization of an intraocular retinoblastoma‐like tumour

In vitro and in vivo growth of an intraocular retinoblastoma‐like tumour in F‐344 rats

Early stage of human adenovirus type 12‐inoculated retinal tissue of F344 newborn rat

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