A highly polymorphic GT-repeat in the bovine STAT5B encoding gene on BTA19

Meyer, Lutz; Brockmann, Gudrun A.; Schwerin, Manfred; Hm, Seyfert

doi:10.1046/j.1365-2052.1999.00323-9.x

Cited by 4 publications

(1 citation statement)

References 1 publication

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We have previously reported a description of the bovine STAT5A cDNA (Schröder et al 1998) and the identification of polymorphic microsatellites in both genes (McCraken et al 1997;Meyer et al 1999) which can be used to link STAT5 with production traits in cattle breeding populations. However, we had detected several copies of pseudogenes among our first genomic isolations, which possessed near-sequence identify over several kilobases to the true STAT5 genes.…”

Section: Introductionmentioning

confidence: 99%

Molecular Characterization of STAT5A- and STAT5B-Encoding Genes Reveals Extended Intragenic Sequence Homogeneity in Cattle and Mouse and Different Degrees of Divergent Evolution of Various Domains

et al. 2000

View full text Add to dashboard Cite

The STAT transcription factors form a family of signal transducers and activators of transcription. We sequenced the bovine STAT5B cDNA and both STAT5-encoding genes, STAT5A and STAT5B, representing the first complete description of any STAT5-encoding gene. DNA fiber FISH hybridization revealed that the genes reside only 40 kbp apart on BTA19. Both genes are segmented into 19 exons and all but two of the homologous exons are of equal size. The genes harbor a central block of nearly identical DNA sequence (97.5% sequence identity over 3373 bp), spanning from intron 5 to intron 9. Isolation and sequencing of the homologous segments from mouse revealed the same unusually high degree of intronic sequence conservation in these segments of the murine STAT5-encoding genes. However, the respective sequences are completely divergent between the two species. A comparison of the inter- and intragenic cDNA sequence preservation at nonsynonymous sites reveals that the DNA-binding domain is under the strongest selection pressure for both intergenic and factor-specific intragenic sequence preservation. The so-called "SH3" segment of the linker domain, in contrast, shows species-specific sequence identity in all but one amino acid residues in both factors, in cattle, human, and mouse. This indicates that the same species-specific selection pressure occurs on the linker domain from both factors, STAT5A and STAT5B. Thus, the comparison of evolutionary selection pressures resting on various domains suggests that the DNA-binding domain might contribute to differential DNA binding of STAT5A and STAT5B factors, while both might interact equally well with other cellular factors through a segment of the linker domain.

show abstract

Section: Introductionmentioning

confidence: 99%

Molecular Characterization of STAT5A- and STAT5B-Encoding Genes Reveals Extended Intragenic Sequence Homogeneity in Cattle and Mouse and Different Degrees of Divergent Evolution of Various Domains

et al. 2000

View full text Add to dashboard Cite

show abstract

The STAT3-encoding gene resides within the 40 kbp gap between the STAT5A- and STAT5B-encoding genes in cattle

et al. 2000

View full text Add to dashboard Cite

A male bovine linkage map for the ADR granddaughter design

Thomsen

Reinsch

et al. 2000

J Animal Breeding Genetics

Self Cite

View full text Add to dashboard Cite

Summary The aim of this paper is to present the construction of a male genetic linkage map as a result of the bovine genome mapping project, which is a common effort of the German cattle breeding federation (ADR), four animal breeding institutes, three blood group laboratories and two animal data and breeding value evaluation centres. In total 20 grandsires with 1074 sires were provided from the German cattle population as reference families, 16 of these paternal half‐sib groups are German Holstein families (DH), three are German Simmental (ST) families, and one is a Brown Swiss family (BS). Of 265 markers included in the linkage map, 248 were microsatellite markers, five were bovine blood group systems, eight SSCP markers and four proteins and enzymes. More than 239 000 genotypes resulted from typing the offspring for the respective markers and these were used for the construction of the map. On average 478 informative meioses were provided from each marker of the map. The summarized map length over all chromosomes was 3135.1 cM with an average interval size of 13.34 cM. About 17, 35.7 and 79.1% of the map intervals showed a maximum genetic distance between the adjacent markers of 5, 10 and 20 cM, respectively. The number of loci ranged from two (pseudoautosomal region of the sex chromosome, BTAY) to 15 (BTA23) with an average of 8.8 markers per chromosome. Comparing the length of the chromosomes shows variation from 49.6 cM for BTA26 to 190.5 cM for BTA1 with a mean of 107.7 cM for all autosomes of the genetic linkage map. It was possible to identify chromosomal discrepancies in locus order and map intervals by comparison with other published maps. The map provided sufficient marker density to serve as a useful tool for a scan of segregating quantitative trait loci.

show abstract

A highly polymorphic GT-repeat in the bovine STAT5B encoding gene on BTA19

Cited by 4 publications

References 1 publication

Molecular Characterization of STAT5A- and STAT5B-Encoding Genes Reveals Extended Intragenic Sequence Homogeneity in Cattle and Mouse and Different Degrees of Divergent Evolution of Various Domains

Molecular Characterization of STAT5A- and STAT5B-Encoding Genes Reveals Extended Intragenic Sequence Homogeneity in Cattle and Mouse and Different Degrees of Divergent Evolution of Various Domains

The STAT3-encoding gene resides within the 40 kbp gap between the STAT5A- and STAT5B-encoding genes in cattle

A male bovine linkage map for the ADR granddaughter design

Contact Info

Product

Resources

About