A highly polymorphic degenerate microsatellite for molecular strain typing of Candida krusei The GenBank accession numbers for the sequences determined in this work are AF326279–AF326292.

Shemer, Revital; Weissman, Ziva; Hashman, N.; Kornitzer, Daniel

doi:10.1099/00221287-147-8-2021

Cited by 24 publications

(23 citation statements)

References 32 publications

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“…The appearance of heterozygosities for some isolates in each of the six gene fragments sequenced confirms previously published suggestions that the genome of C. krusei is diploid (7,26).…”

Section: Discussionsupporting

confidence: 71%

“…We use MLST to show that patients tend to be colonized and/or infected with the same strain type and that the population structure of C. krusei consists of four clusters of closely related strain subtypes, with one comprising just two isolates that were markedly different from the other isolates tested. The results also confirm that C. krusei is diploid (7,26).…”

supporting

confidence: 77%

“…Shemer et al found 17 haplotypes of C. krusei, based on microsatellite typing of a highly polymorphic locus, among 50 isolates (26). Both studies therefore indicated a high inherent level of diversity among isolates of C. krusei.…”

Section: Discussionmentioning

confidence: 95%

“…MLST facilitates determinations of population structures and epidemiological correlates of properties, such as geographical and anatomical origins of isolates and their transmission within and between patients (2,6,29). C. krusei isolates have previously been typed by electrophoretic karyotyping (5), restriction fragment length polymorphism analysis (5), DNA fingerprinting (4), and microsatellite polymorphism approaches (26). In this study, we present a scheme for MLST with C. krusei.…”

mentioning

confidence: 99%

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Strain Typing and Determination of Population Structure ofCandida kruseiby Multilocus Sequence Typing

et al. 2007

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A multilocus sequence typing (MLST) scheme for Candida krusei was devised, based on sequencing of six gene fragments of the species. The existence of heterozygous results for each of the six fragments sequenced confirms that C. krusei is diploid for at least part of its genome. The C. krusei MLST scheme had a discriminatory index of 0.998, making this system ideal for strain typing of C. krusei clinical isolates. MLST data for 122 independent C. krusei isolates from a range of geographical sources were analyzed by eBURST, structure, and the unweighted-pair group method using average linkages to derive a population structure comprising four subtype strain clusters. There was no evidence of geographical associations with particular subtypes. Data for pairs of isolates from seven patients showed that each patient was colonized and/or infected with strain types that were indistinguishable by MLST. The C. krusei MLST database can be accessed online at http://pubmlst.org/ckrusei/.

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“…The appearance of heterozygosities for some isolates in each of the six gene fragments sequenced confirms previously published suggestions that the genome of C. krusei is diploid (7,26).…”

Section: Discussionsupporting

confidence: 71%

supporting

confidence: 77%

Section: Discussionmentioning

confidence: 95%

mentioning

confidence: 99%

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Strain Typing and Determination of Population Structure ofCandida kruseiby Multilocus Sequence Typing

et al. 2007

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“…The microsatellite genotyping of several yeast species, namely, Saccharomyces cerevisiae (14), C. albicans (3,4,33), C. krusei (34), and C. glabrata (13), has been described.…”

Section: Discussionmentioning

confidence: 99%

New Polymorphic Microsatellite Markers Able To Distinguish among Candida parapsilosis Sensu Stricto Isolates

et al. 2010

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Among the Candida species causing bloodstream infections, Candida parapsilosis is one of the most frequently isolated. The objective of the present work was the identification of new microsatellite loci able to distinguish among C. parapsilosis isolates. DNA sequences with trinucleotide repeats were selected from the C. parapsilosis genome database. PCR primer sets flanking the microsatellite repeats were designed and tested with 20 independent isolates. On the basis of the amplification efficiency, specificity, and observed polymorphism, four of the sequences were selected for strain typing. Two hundred thirty-three independent C. parapsilosis sensu stricto isolates were genotyped by using these markers. The polymorphic loci exhibited from 20 to 42 alleles and 39 to 92 genotypes. In a multiplex analysis, 192 genotypes were obtained and the combined discriminatory power of the four microsatellites was 0.99. Reproducibility was demonstrated by submission of subcultures of 4 isolates each, in triplicate, interspersed with unique numbers among a group of 30 isolates for blind testing. Comparison of the genotypes obtained by microsatellite analysis and those obtained by randomly amplified polymorphic DNA analysis, restriction fragment length polymorphism analysis, and internal transcribed sequence grouping was performed and showed that the microsatellite method could distinguish individual isolates; none of the other methods could do that. Related species, C. orthopsilosis and C. metapsilosis, were not confused with C. parapsilosis sensu stricto. These new microsatellites are a valuable tool for use for the differentiation of C. parapsilosis sensu stricto strains, vital in epidemiology to answer questions of strain relatedness and determine pathways of transmission.

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Current Awareness

2002

Yeast

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In order to keep subscribers up‐to‐date with the latest developments in their field, this current awareness service is provided by John Wiley & Sons and contains newly‐published material on yeasts. Each bibliography is divided into 10 sections. 1 Books, Reviews & Symposia; 2 General; 3 Biochemistry; 4 Biotechnology; 5 Cell Biology; 6 Gene Expression; 7 Genetics; 8 Physiology; 9 Medical Mycology; 10 Recombinant DNA Technology. Within each section, articles are listed in alphabetical order with respect to author. If, in the preceding period, no publications are located relevant to any one of these headings, that section will be omitted. (4 weeks journals ‐ search completed 14th. Nov. 2001)

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A highly polymorphic degenerate microsatellite for molecular strain typing of Candida krusei The GenBank accession numbers for the sequences determined in this work are AF326279–AF326292.

Cited by 24 publications

References 32 publications

Strain Typing and Determination of Population Structure ofCandida kruseiby Multilocus Sequence Typing

Strain Typing and Determination of Population Structure ofCandida kruseiby Multilocus Sequence Typing

New Polymorphic Microsatellite Markers Able To Distinguish among Candida parapsilosis Sensu Stricto Isolates

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