A highly pathogenic recombinant infectious bronchitis virus with adaptability in cultured cells

Huang, Mengjiao; Liu, Yuan; Zou, Chuangchao; Tan, Yazhuo; Han, Zhenling; Xue, Chunyi; Cao, Yongchang

doi:10.1016/j.virusres.2020.198229

Cited by 5 publications

(3 citation statements)

References 35 publications

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“…Similar to other coronaviruses, IBV generally exhibits restricted cell and tissue tropism, depending upon the S glycoprotein of individual strains (Sánchez et al, 1999;Tekes et al, 2010). Some IBV strains are able to replicate in primary chicken cells, such as chick kidney (CK) cells (Oade et al, 2019) and chick embryo fibroblasts (DF1) (Harrison et al, 2007;Huang et al, 2021). Interestingly, the Beaudette strain shows an extended host range with the ability to replicate in Vero and a number of human and other animal cell lines (Fang et al, 2005;Tay et al, 2012), as well as in baby hamster kidney (BHK-21) cell line after passaging in embryonated eggs (Otsuki et al, 1979;Fang et al, 2005).…”

Section: Discussionmentioning

confidence: 99%

Establishment and Cross-Protection Efficacy of a Recombinant Avian Gammacoronavirus Infectious Bronchitis Virus Harboring a Chimeric S1 Subunit

et al. 2022

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Infectious bronchitis virus (IBV) is a gammacoronavirus that causes a highly contagious disease in chickens and seriously endangers the poultry industry. A diversity of serotypes and genotypes of IBV have been identified worldwide, and the currently available vaccines do not cross-protect. In the present study, an efficient reverse genetics technology based on Beaudette-p65 has been used to construct a recombinant IBV, rIBV-Beaudette-KC(S1), by replacing the nucleotides 21,704–22,411 with the corresponding sequence from an isolate of QX-like genotype KC strain. Continuous passage of this recombinant virus in chicken embryos resulted in the accumulation of two point mutations (G21556C and C22077T) in the S1 region. Further studies showed that the T248S (G21556C) substitution may be essential for the adaptation of the recombinant virus to cell culture. Immunization of chicks with the recombinant IBV elicited strong antibody responses and showed high cross-protection against challenges with virulent M41 and a QX-like genotype IBV. This study reveals the potential of developing rIBV-Beau-KC(S1) as a cell-based vaccine with a broad protective immunity against two different genotypes of IBV.

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Section: Discussionmentioning

confidence: 99%

Establishment and Cross-Protection Efficacy of a Recombinant Avian Gammacoronavirus Infectious Bronchitis Virus Harboring a Chimeric S1 Subunit

et al. 2022

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“…With this feature, the cell line is well suited for isolating avian leucosis viruses [173]. An infectious bronchitis virus that causes kidney lesions and respiratory signs can induce a CPE in DF-1 cells, but without being passaged that is different to the observation in QT-35 cells, in which passaging could be achieved albeit with low titers [159,174]. Similarly, higher titers were obtained with primary chicken embryo fibroblasts as compared to DF-1 cells, even though both cell culture systems multiplied IBDV and NDV [175].…”

Section: Virologymentioning

confidence: 99%

Diagnosing Infectious Diseases in Poultry Requires a Holistic Approach: A Review

Liebhart

Bilić

Grafl

et al. 2023

Poultry

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Controlling infectious diseases is vital for poultry health and diagnostic methods are an indispensable feature to resolve disease etiologies and the impact of infectious agents on the host. Although the basic principles of disease diagnostics have not changed, the spectrum of poultry diseases constantly expanded, with the identification of new pathogens and improved knowledge on epidemiology and disease pathogenesis. In parallel, new technologies have been devised to identify and characterize infectious agents, but classical methods remain crucial, especially the isolation of pathogens and their further characterization in functional assays and studies. This review aims to highlight certain aspects of diagnosing infectious poultry pathogens, from the farm via the diagnostic laboratory and back, in order to close the circle. By this, the current knowledge will be summarized and future developments will be discussed in the context of applied state-of-the-art techniques. Overall, a common challenge is the increasing demand for infrastructure, skills and expertise. Divided into separate chapters, reflecting different disciplines, daily work implies the need to closely link technologies and human expertise in order to improve bird health, the production economy and to implement future intervention strategies for disease prevention.

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“…With variations between IBV strains and an increase in the number of genotypes and serotypes, some studies have found the potential of a few isolates to adapt to passaged cell lines. For instance, the recombinant strain isolated by Huang et al ( 10 ) was able to cause lesions not only in primary CEK cells but also in DF-1 cells, with N gene upregulation and N protein expression detected except during successive passages. This highlights the importance of studying the biological properties of new strains in addition to ongoing epidemiological investigations and molecular surveillance of IBV.…”

Section: Introductionmentioning

confidence: 99%

Differential analysis of IBV-infected primary chicken embryonic fibroblasts and immortalized DF-1

Yang,

Gong,

Liu

et al. 2024

Microbiol Spectr

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Infectious bronchitis virus (IBV), the causative agent of infectious bronchitis, is responsible for major economic losses in the poultry industry worldwide. While IBVs can usually be passaged in primary chicken embryonic fibroblasts (CEFs), most of the wild ones cannot adapt to passaged cell lines. In this study, the wild strain CK/CH/MY/2020 was used to infect primary CEF and immortalize DF-1 CEF cells. Results indicated that IBV was able to cause lesions and pass onto CEF, but not DF-1. Indeed, the virus could enter DF-1 cells and synthesize the associated structural gene but could not assemble into complete viral particles for release. Furthermore, transcriptome sequencing analysis showed significant differences in gene expression between CEF and DF-1 cells after viral infection, although the corresponding antiviral responses could be activated in both cell types. The biggest difference was in terms of the amino acid biosynthesis pathway and the cytokine receptor interaction pathway, which were significantly and specifically activated in CEF. This could actually explain why intact viruses can be assembled but not in DF-1. In addition, SLBP and P2RX7 affect the replication of IBV’s structural genes to some extent. Overall, IBV can enter CEF and DF-1 cells, but the complex intracellular cytokine interactions affect the assembly and release of viral particles. The insight will be useful for the study of IBV through in vitro transmission and pathogenesis. IMPORTANCE Infectious bronchitis virus (IBV) is responsible for high morbidity and mortality as well as substantial economic losses worldwide. Transcriptome sequencing of IBV-infected chicken embryonic fibroblast and DF-1 cells revealed that the virus elicits antiviral immunity in cells after viral infection, but IBV cannot activate DF-1 cells to produce sufficient amounts of viral structures to assemble into complete virions, which may be caused by the interactions between cytokines. The study of IBV cellular adaptations is important for vaccine development and investigation of the pathogenesis of IBV.

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A highly pathogenic recombinant infectious bronchitis virus with adaptability in cultured cells

Cited by 5 publications

References 35 publications

Establishment and Cross-Protection Efficacy of a Recombinant Avian Gammacoronavirus Infectious Bronchitis Virus Harboring a Chimeric S1 Subunit

Establishment and Cross-Protection Efficacy of a Recombinant Avian Gammacoronavirus Infectious Bronchitis Virus Harboring a Chimeric S1 Subunit

Diagnosing Infectious Diseases in Poultry Requires a Holistic Approach: A Review

Differential analysis of IBV-infected primary chicken embryonic fibroblasts and immortalized DF-1

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