A Highly Efficient Colorimetric Immunoassay Using a Nanocomposite Entrapping Magnetic and Platinum Nanoparticles in Ordered Mesoporous Carbon

Mi, Kim Young; Jw, Lee; Park, Hyun Gyu

doi:10.1002/adhm.201300100

Cited by 59 publications

(24 citation statements)

References 37 publications

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“…utilised the highly active peroxidase mimics, MNPs and platinum NPs, within porous carbon, as a signalling element for labelling with antibodies for the detection of human epidermal growth factor with a detection limit as low as 1.5 ng/mL when analysed using UV-vis spectroscopy. 19 The catalytic activity of Au NPs conjugated to antibodies has also been applied in an ELISA format. However, Wang et al reported that the catalytic activity was inhibited due to the addition of the protein molecules, but could be increased by the deposition of Au due to the formation of a new Au shell.…”

Section: Introductionmentioning

confidence: 99%

A novel nanozyme assay utilising the catalytic activity of silver nanoparticles and SERRS

Sloan-Dennison

Laing

Shand

et al. 2017

Analyst

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aArtificial enzymes have become an increasingly interesting area of research due to their many advantages over natural protein enzymes which are expensive, difficult to isolate and unable to stand harsh environments. An important area of this research involves using metal nanoparticles as artificial enzymes, known as nanozymes, which exhibit peroxidase-like activity enabling them to catalyse the oxidation of substrates such as 3,3',5,5'-tetramethylbenzidine (TMB) in the presence of hydrogen peroxide (H2O2), giving a colorimetric response. Here we exploit the catalytic activity of silver nanoparticles (Ag NPs) in a surface based silver-linked immunosorbent assay (SLISA) to detect human C-reactive protein (CRP), an inflammatory marker. Ag NPs were conjugated to antibodies with specific recognition for the corresponding target antigenic molecule, CRP, and the Ag NPs were used to catalyse the oxidation of TMB by H2O2. The resulting coloured oxidation product was detected using SERRS. We demonstrate that Ag NPs can replace the enzymes used in a conventional ELISA and a detection limit of 1.09 ng/mL of CRP can be achieved. It indicates the promise for SLISAs for biomarker detection and opens the way for further assays of this nature to be created. This novel assay has the potential to be optimised to detect lower levels of CRP and can be further extended for the sensitive and specific detection of other relevant biomarkers.

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Section: Introductionmentioning

confidence: 99%

A novel nanozyme assay utilising the catalytic activity of silver nanoparticles and SERRS

Sloan-Dennison

Laing

Shand

et al. 2017

Analyst

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“…For example, Qu et al, designed a heterogeneous palladium nanozyme that could effectively mediate the bioorthogonal reactions in situ through light and thus realized the specific imaging of mitochondria in living cells (Figures 2B,C). Beside CTCs and organelle imaging detection, there are also several other nanozymes-based colorimetric methods for specific disease imaging, including jaundice (Santhosh et al, 2014), acquired immune deficiency syndrome , diabetes (Tianran et al, 2014), infectious disease (Kim et al, 2014;Duan et al, 2015), and neurodegenerative disease (Wang C. I. et al, 2012;Farhadi et al, 2014). Thus, compared to traditional methods (such as PCR, cell flow cytometry, and ELISA), nanozymes methods exhibit more broaden prospect for live cell and organelle imaging because nanozyme assay is more fast, cost-effective and much easier to operate.…”

Section: Nanozymes For Live Cell and Organelle Imagingmentioning

confidence: 99%

Nanozymes: A New Disease Imaging Strategy

Wang

Hong

et al. 2020

Front. Bioeng. Biotechnol.

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Nanozymes are nanomaterials with intrinsic enzyme-like properties. They can specifically catalyze substrates of natural enzymes under physiological condition with similar catalytic mechanism and kinetics. Compared to natural enzymes, nanozymes exhibit the unique advantages including high catalytic activity, low cost, high stability, easy mass production, and tunable activity. In addition, as a new type of artificial enzymes, nanozymes not only have the enzyme-like catalytic activity, but also exhibit the unique physicochemical properties of nanomaterials, such as photothermal properties, superparamagnetism, and fluorescence, etc. By combining the unique physicochemical properties and enzyme-like catalytic activities, nanozymes have been widely developed for in vitro detection and in vivo disease monitoring and treatment. Here we mainly summarized the applications of nanozymes for disease imaging and detection to explore their potential application in disease diagnosis and precision medicine.

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“…10, 15 MNP are promising nanocatalysts because of their magnetic properties, high catalytic activity, and adjustability through nanoscale modifications 10, 16–19 , suggesting possible uses in immunoassays 10, 13, 20, 21 , organic pollutant degradation 12, 22–26 , glucose detection 17, 18, 27–31 and cancer diagnostics. 14 Although it has been reported that MNP alone or combined with H 2 O 2 can prevent biofilm formation by inhibiting bacterial growth, 32, 33 there are no reports regarding the efficacy of an MNP-H 2 O 2 system on biofilm destruction and killing of bacteria resident within biofilms—a much more difficult medical challenge.…”

mentioning

confidence: 99%

Ferromagnetic nanoparticles with peroxidase-like activity enhance the cleavage of biological macromolecules for biofilm elimination

et al. 2014

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Hydrogen peroxide (H2O2) is a “green chemical” that has various cleaning and disinfectant uses, including as an anti-bacterial agent for hygienic and medical treatments. However, its efficacy is limited against biofilm-producing bacteria, because of poor penetration of the protective, organic matrix. Here we show new applications for ferromagnetic nanoparticles (Fe3O4, MNP) with peroxidase-like activity in potentiating the efficacy of H2O2 in biofilm degradation and prevention. Our data show that MNP enhanced oxidative cleavage of biofilm components (model nucleic acids, proteins, and oligosaccharides) in the presence of H2O2. When challenged with live, biofilm-producing bacteria, the MNP-H2O2 system efficiently broke down existing biofilm and prevented new biofilm from forming, killing both planktonic bacteria and those within biofilm. By enhancing oxidative cleavage of various substrates, the MNP-H2O2 system provides a novel strategy for biofilm elimination, and other applications utilizing oxidative breakdown.

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A Highly Efficient Colorimetric Immunoassay Using a Nanocomposite Entrapping Magnetic and Platinum Nanoparticles in Ordered Mesoporous Carbon

Cited by 59 publications

References 37 publications

A novel nanozyme assay utilising the catalytic activity of silver nanoparticles and SERRS

A novel nanozyme assay utilising the catalytic activity of silver nanoparticles and SERRS

Nanozymes: A New Disease Imaging Strategy

Ferromagnetic nanoparticles with peroxidase-like activity enhance the cleavage of biological macromolecules for biofilm elimination

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