A highly efficient biosensor based on MAPLE deposited hemoglobin on LPGs around phase matching turning point

Dyankov, Georgi; Eftimov, Tinko; Malinowski, N.; Belina, E.; Kisov, Hristo; Mikulic, Predrag; Bock, Wojtek J.

doi:10.1016/j.optlastec.2019.105907

Cited by 18 publications

(11 citation statements)

References 9 publications

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“…The performance of directly immobilized Hb is confirmed by the achieved very sensitive glucose detection with low detection limit 0.1 mmol/L [ 10 ]. We cannot compare our results with other reports, since we were first to use hemoglobin as a ligand for glucose detection.…”

Section: Resultsmentioning

confidence: 99%

“…Heme proteins, such as hemoglobin (Hb) and myoglobin (Mb), have been widely used for sensing non-organic compounds like NO 2 [ 1 ], NO 2 and H 2 O 2 [ 2 , 3 ], C 8 H 8 O 3 [ 4 ], NO [ 5 , 6 ], CO [ 7 , 8 ] and HS [ 9 ]. Reference [ 10 ] has reported a biosensor with Hb as the recognition element (ligand) for sensing organic compounds (glucose).…”

Section: Introductionmentioning

confidence: 99%

“…Our preliminary results proved the feasibility of MAPLE for direct Hb/Mb immobilization—we showed that the main functional groups of proteins are present in the deposited film. In [ 10 ] we showed the sensing efficiency of directly immobilized Hb, while in [ 31 ] we provided supplementary information regarding the immobilization technique.…”

Section: Introductionmentioning

confidence: 99%

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A Surface Plasmon Resonance Biosensor Based on Directly Immobilized Hemoglobin and Myoglobin

Dyankov

Borisova

Belina

et al. 2020

Sensors

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Immobilization of proteins on a surface plasmon resonance (SPR) transducer is a delicate procedure since loss of protein bioactivity can occur upon contact with the untreated metal surface. Solution to the problem is the use of an immobilization matrix having a complex structure. However, this is at the expense of biosensor selectivity and sensitivity. It has been shown that the matrix-assisted pulsed laser evaporation (MAPLE) method has been successfully applied for direct immobilization (without a built-in matrix) of proteins, preserving their bioactivity. So far, MAPLE deposition has not been performed on a gold surface as required for SPR biosensors. In this paper we study the impact of direct immobilization of heme proteins (hemoglobin (Hb) and myoglobin (Mb)) on their bioactivity. For the purpose, Hb and Mb were directly immobilized by MAPLE technique on a SPR transducer. The bioactivity of the ligands immobilized in the above-mentioned way was assessed by SPR registration of the molecular reactions of various Hb/Mb functional groups. By SPR we studied the reaction between the beta chain of the Hb molecule and glucose, which shows the structural integrity of the immobilized Hb. A supplementary study of films deposited by FTIR and AFM was provided. The experimental facts showed that direct immobilization of an intact molecule was achieved.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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A Surface Plasmon Resonance Biosensor Based on Directly Immobilized Hemoglobin and Myoglobin

Dyankov

Borisova

Belina

et al. 2020

Sensors

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“…In this process, hemoglobin is directly immobilized on the fiber structure through a matrix-assisted pulsed-laser evaporation method, resulting in spectral shifts due to the change in refractive index, which is correlated to the glucose concentration in the sample. The device can show reliable performance for glucose concentration as low as 0.1 mmol/L with an LOD of 10 μM [ 56 ].…”

Section: Recent Developments In Various Biological Fluid-based Glucos...mentioning

confidence: 99%

Recent Advancement in Biofluid-Based Glucose Sensors Using Invasive, Minimally Invasive, and Non-Invasive Technologies: A Review

Reddy

Agarwal

et al. 2022

Nanomaterials

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Biosensors have potentially revolutionized the biomedical field. Their portability, cost-effectiveness, and ease of operation have made the market for these biosensors to grow rapidly. Diabetes mellitus is the condition of having high glucose content in the body, and it has become one of the very common conditions that is leading to deaths worldwide. Although it still has no cure or prevention, if monitored and treated with appropriate medication, the complications can be hindered and mitigated. Glucose content in the body can be detected using various biological fluids, namely blood, sweat, urine, interstitial fluids, tears, breath, and saliva. In the past decade, there has been an influx of potential biosensor technologies for continuous glucose level estimation. This literature review provides a comprehensive update on the recent advances in the field of biofluid-based sensors for glucose level detection in terms of methods, methodology and materials used.

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“…The DTP working conditions can be fine-tuned by etching the cladding [11]. DTP-LPGs are the most sensitive to RI changes, which makes them suitable for biosensing applications [12,13].…”

Section: Introductionmentioning

confidence: 99%

Long-Period Gratings and Microcavity In-Line Mach Zehnder Interferometers as Highly Sensitive Optical Fiber Platforms for Bacteria Sensing

Eftimov

Janik

Koba

et al. 2020

Sensors

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Selected optical fiber sensors offer extraordinary sensitivity to changes in external refractive (RI), which make them promising for label-free biosensing. In this work the most sensitive ones, namely long-period gratings working at (DTP-LPG) and micro-cavity in-line Mach-Zehnder interferometers (µIMZI) are discussed for application in bacteria sensing. We describe their working principles and RI sensitivity when operating in water environments, which is as high as 20,000 nm/RIU (Refractive index unit) for DTP-LPGs and 27,000 nm/RIU for µIMZIs. Special attention is paid to the methods to enhance the sensitivity by etching and nano-coatings. While the DTP-LPGs offer a greater interaction length and sensitivity to changes taking place at their surface, the µIMZIs are best suited for investigations of sub-nanoliter and picoliter volumes. The capabilities of both the platforms for bacteria sensing are presented and compared for strains of Escherichia coli, lipopolysaccharide E. coli, outer membrane proteins of E. coli, and Staphylococcus aureus. While DTP-LPGs have been more explored for bacteria detection in 102–106 Colony Forming Unit (CFU)/mL for S. aureus and 103–109 CFU/mL for E. coli, the µIMZIs reached 102–108 CFU/mL for E. coli and have a potential for becoming picoliter bacteria sensors.

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A highly efficient biosensor based on MAPLE deposited hemoglobin on LPGs around phase matching turning point

Cited by 18 publications

References 9 publications

A Surface Plasmon Resonance Biosensor Based on Directly Immobilized Hemoglobin and Myoglobin

A Surface Plasmon Resonance Biosensor Based on Directly Immobilized Hemoglobin and Myoglobin

Recent Advancement in Biofluid-Based Glucose Sensors Using Invasive, Minimally Invasive, and Non-Invasive Technologies: A Review

Long-Period Gratings and Microcavity In-Line Mach Zehnder Interferometers as Highly Sensitive Optical Fiber Platforms for Bacteria Sensing

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