A Highly Conserved Interspecies V H in the Human Genome

Vogel, Monique; Tschopp, Cornelia; Bobrzynski, Tomasz; Fux, Michaela; Stadler, Michael; Miescher, Sylvia; Stadler, Beda M.

doi:10.1016/j.jmb.2004.06.011

Cited by 3 publications

(1 citation statement)

References 54 publications

(48 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To generate CBM␣8 as an Ig, the H and L chain genes were fused to the IgG Fc by cloning to modified (33) vectors from the V H and VKExpress system (34), and the constructs were verified by sequencing. Human embryonic kidney cells (HEK 293; American Type Culture Collection; CRL-1573; adjusted to suspension culture) were cotransfected with the H and L chain constructs (Swiss Federal Institute of Technology) and cultured in EX-CELL 293 serum-free medium (JRH Biosciences) for 5-6 days.…”

Section: Generation Of Full-length Cbm␣8mentioning

confidence: 99%

A High-Affinity Natural Autoantibody from Human Cord Blood Defines a Physiologically Relevant Epitope on the FcεRIα

Bobrzynski

Fux

Vogel

et al. 2005

The Journal of Immunology

Self Cite

View full text Add to dashboard Cite

Natural Abs represent the indigenous immune repertoire and are thus present at birth and persist throughout life. Previously, human autoantibodies to the α domain of the high-affinity IgE receptor (FcεRIα) have been isolated from Ab libraries derived from normal donors and patients with chronic urticaria. To investigate whether these anti-FcεRIα Abs are present in the germline repertoire, we constructed a phage Fab display library from human cord blood, which represents the naive immune repertoire before exposure to exogenous Ags. All isolated clones specific to the FcεRIα had the same sequence. This single IgM Ab, named CBMα8, was strictly in germline configuration and had high affinity and functional in vitro anaphylactogenic activity. Inhibition experiments indicated an overlapping epitope on the FcεRIα recognized by both CBMα8 and the previously isolated anti-FcεRIα Abs from autoimmune and healthy donors. This common epitope on FcεRIα coincides with the binding site for IgE. Affinity measurements demonstrated the presence of Abs showing CBMα8-like specificity, but with a significantly lower affinity in i.v. Ig, a therapeutic multidonor IgG preparation. We propose a hypothesis of escape mutants, whereby the resulting lower affinity IgG anti-FcεRIα Abs are rendered less likely to compete with IgE for binding to FcεRIα.

show abstract

Section: Generation Of Full-length Cbm␣8mentioning

confidence: 99%

A High-Affinity Natural Autoantibody from Human Cord Blood Defines a Physiologically Relevant Epitope on the FcεRIα

Bobrzynski

Fux

Vogel

et al. 2005

The Journal of Immunology

Self Cite

View full text Add to dashboard Cite

show abstract

Survey of the year 2004 commercial optical biosensor literature

Rich

Myszka

2005

J of Molecular Recognition

114

View full text Add to dashboard Cite

The year 2004 represents a milestone for the biosensor research community: in this year, over 1000 articles were published describing experiments performed using commercially available systems. The 1038 papers we found represent a $ 10% increase over the past year and demonstrate that the implementation of biosensors continues to expand at a healthy pace. We evaluated the data presented in each paper and compiled a 'top 10' list. These 10 articles, which we recommend every biosensor user reads, describe wellperformed kinetic, equilibrium and qualitative/screening studies, provide comparisons between binding parameters obtained from different biosensor users, as well as from biosensor-and solution-based interaction analyses, and summarize the cutting-edge applications of the technology. We also re-iterate some of the experimental pitfalls that lead to sub-optimal data and over-interpreted results. We are hopeful that the biosensor community, by applying the hints we outline, will obtain data on a par with that presented in the 10 spotlighted articles. This will ensure that the scientific community at large can be confident in the data we report from optical biosensors.

show abstract