A highly branched 1,3-β-d-glucan extracted from Aureobasidium pullulans induces cytokine production in DBA/2 mouse-derived splenocytes

Tada, Rui; Yoshikawa, Masashi; Kuge, Takao; Tanioka, Asuka; Ishibashi, Keiichiro; Adachi, Yoshiyuki; Tsubaki, Kazufumi; Ohno, Naohito

doi:10.1016/j.intimp.2009.08.014

Cited by 21 publications

(15 citation statements)

References 22 publications

(44 reference statements)

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“…(9,10) We also revealed that AP-FBG could induce the production of cytokines in a splenocyte culture system derived from DBA/2 mice, which are highly sensitive to β-d-glucan. (11)(12)(13)(14)(15) However, the mechanism(s) by which AP-FBG, which possesses a characteristic high 6-branching structural feature, induced the production of cytokines by immune cells has not been determined.…”

Section: Research Articlementioning

confidence: 99%

Granulocyte macrophage colony–stimulating factor is required for cytokine induction by a highly 6-branched 1,3-β-d-glucan fromAureobasidium pullulansin mouse-derived splenocytes

Tada¹,

Yoshikawa²,

Kuge³

et al. 2010

Immunopharmacology and Immunotoxicology

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We have previously obtained and elucidated the precise structure of a highly branched 1,3-β-D-glucan (with 6-monoglucopyranosyl side chains), Aureobasidium pullulans-fermented β-D-glucan (AP-FBG), from the fungus A. pullulans. However, the mechanism(s) of the effects of AP-FBG on in vitro mouse primary cells have not been analyzed in detail. Herein, we report that the induction of cytokines by AP-FBG was dependent on the existence of a granulocyte macrophage colony-stimulating factor (GM-CSF); this is similar way to be a typical 1,3-β-D-glucan from Sparassis crispa (SCG), which is a 1,3-β-D-glucopyranosyl backbone with single 1,6-β-D-glucopyranosyl side branching units every three residues. In other words, the production of cytokines in DBA/2-mouse-derived splenocytes by AP-FBG was completely hampered by an anti-GM-CSF neutralizing monoclonal antibody. Furthermore, the addition of exogenous GM-CSF to C57BL/6-derived splenocytes, which are less sensitive to AP-FBG, induced the production of cytokines by AP-FBG. Therefore, GM-CSF is indispensable for the induction of cytokines by AP-FBG in mouse-derived splenocytes. This finding has provided a new insight into our understanding of the actions of β-D-glucan but will also aid in the design and development of more effective β-D-glucan agents.

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Section: Research Articlementioning

confidence: 99%

Granulocyte macrophage colony–stimulating factor is required for cytokine induction by a highly 6-branched 1,3-β-d-glucan fromAureobasidium pullulansin mouse-derived splenocytes

Tada¹,

Yoshikawa²,

Kuge³

et al. 2010

Immunopharmacology and Immunotoxicology

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“…Chen and Seviour (25) reported correlations between the immunological responses of β-glucan and its molecular structure, size, branching frequency, structural modification, conformation, and solubility. Tada et al (26) reported that β-glucan extracted from the fungus Aureobasidium pullulans, remarkably induces robust cytokine production in DBA/2 mouse-derived splenocytes, and their biological effects may depend on their structures. Moreover, Lehmann and Kunze (27) claimed that immunological responses by β-glucan were more effective at low molecular weight (1 to 30 kDa) than at high molecular weight (>100 kDa) in clinical investigation.…”

Section: Resultsmentioning

confidence: 99%

Low molecular weight β-glucan stimulates doxorubicin-induced suppression of immune functions in mice

Sung

Byun

Song

et al. 2012

Food Sci Biotechnol

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“…Next, to rule out the presence of contaminating endotoxin in the maleylated-BSA samples, we examined whether PMB, a well-known endotoxin inhibitor, affected TNF-α production in our macrophages [18,19]. We observed that the PMB treatment did not affect maleylated-BSA-induced production of TNF-α, whereas LPS treatment completely abrogated TNF-α production (Figure 2).…”

Section: Effect Of Pmb On Maleylated-bsa-induced Tnf-α Productionmentioning

confidence: 96%

Maleylated-BSA induces TNF-α production through the ERK and NF-κB signaling pathways in murine RAW264.7 macrophages

Tada¹,

Koide²,

Yamamuro³

et al. 2013

OJI

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Ligands for macrophage scavenger receptors are reported to induce a wide range of host cell responses, including the production of inflammatory cytokines; however, the underlying mechanisms have not yet been fully understood and which remain obscure. In this study, we have examined the effect of maleylated bovine serum albumin (maleylated-BSA), a well-known ligand of the scavenger receptor, on the murine macrophage cell line RAW264.7. Maleylated-BSA strongly induced the production of tumor necrosis factor-α (TNF-α) and induced phosphorylation of extracellular signal-regulated kinase (ERK) and NF-kB p65. We also observed that maleylated-BSA-induced TNF-α production was blocked by the ERK inhibitor U0126. Together, these data demonstrates that maleylated-BSAinduced production of TNF-α requires the ERK/NF-κB signaling cascade in murine RAW-264.7 macrophages.

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A highly branched 1,3-β-d-glucan extracted from Aureobasidium pullulans induces cytokine production in DBA/2 mouse-derived splenocytes

Cited by 21 publications

References 22 publications

Granulocyte macrophage colony–stimulating factor is required for cytokine induction by a highly 6-branched 1,3-β-d-glucan fromAureobasidium pullulansin mouse-derived splenocytes

Granulocyte macrophage colony–stimulating factor is required for cytokine induction by a highly 6-branched 1,3-β-d-glucan fromAureobasidium pullulansin mouse-derived splenocytes

Low molecular weight β-glucan stimulates doxorubicin-induced suppression of immune functions in mice

Maleylated-BSA induces TNF-α production through the ERK and NF-κB signaling pathways in murine RAW264.7 macrophages

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