A Higher CD105-Assessed Microvessel Density and Worse Prognosis in Elderly Patients With Laryngeal Carcinoma

Marioni, Gino; Staffieri, Alberto; Manzato, Enzo; Ralli, Giovanni; Lionello, Marco; Giacomelli, Luciano; Prosenikliev, Vlatko; Marchese‐Ragona, Rosario; Busnardo, Alessandra; Bolzetta, Francesco; Blandamura, Stella

doi:10.1001/archoto.2010.244

Cited by 16 publications

(11 citation statements)

References 19 publications

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“…Twenty‐one reports were finally identified with MVD measurements in patients with HNSCC, whereas 2 of which duplicated study data . Of all the remaining 19 reports, 7 were excluded: 4 studies lacked informative clinical data, 2 were related to disease‐free survival (DFS), and 1 only showed recurrence‐free survival (Figure A). A total of 55 articles evaluating the association between LVD and HNSCC were retrieved, and we evaluated 16 reports in full text.…”

Section: Resultsmentioning

confidence: 99%

Intratumoral vessel density as prognostic factors in head and neck squamous cell carcinoma: A meta‐analysis of literature

Liu

Liang

et al. 2013

Head & Neck

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Section: Resultsmentioning

confidence: 99%

Intratumoral vessel density as prognostic factors in head and neck squamous cell carcinoma: A meta‐analysis of literature

Liu

Liang

et al. 2013

Head & Neck

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“…It is a well-characterized angiogenic marker that is upregulated during angiogenesis, and is overexpressed in vascular endothelium in malignancies including ovarian, leukemia, gastrointestinal stromal tumors (GIST), melanoma, and laryngeal cancers, but is rarely expressed in non-endothelial cells. 2-3 It is a co-receptor of TGFBR2 that binds TGF-B and is an important mediator of fetal vascular/endothelial development. 4 Recently, anti-angiogenic agents have received extensive attention as new therapeutic modalities, and CD105 has become an additional target by which intratumoral angiogenesis may be targeted.…”

Section: Introductionmentioning

confidence: 99%

Endoglin (CD105) Contributes to Platinum Resistance and Is A Target for Tumor-Specific Therapy in Epithelial Ovarian Cancer

Ziebarth

Nowsheen

Steg

et al. 2013

Clinical Cancer Research

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Purpose Endoglin (ENG, CD105) is a membranous protein overexpressed in tumor-associated endothelial cells, chemoresistant populations of ovarian cancer cells, and potentially stem cells. Our objective was to evaluate the effects and mechanisms of targeting endoglin in ovarian cancer. Experimental Design Global and membranous endoglin expression was evaluated in multiple ovarian cancer lines. In vitro, the effects of siRNA-mediated endoglin knockdown with and without chemotherapy were evaluated by MTT assay, cell-cycle analysis, alkaline comet assay, γ-H2AX foci formation, and qPCR. In an orthotopic mouse model, endoglin was targeted with chitosan-encapsulated siRNA with and without carboplatin. Results Endoglin expression was surprisingly predominantly cytoplasmic, with a small population of surface-positive cells. Endoglin inhibition decreased cell viability, increased apoptosis, induced double-stranded DNA damage, and increased cisplatin sensitivity. Targeting endoglin downregulates expression of numerous DNA repair genes, including BARD1, H2AFX, NBN, NTHL1, and SIRT1. BARD1 was also associated with platinum resistance, and was induced by platinum exposure. In vivo, anti-endoglin treatment decreased tumor weight in both ES2 and HeyA8MDR models when compared to control (35-41% reduction, p<0.05). Endoglin inhibition with carboplatin was associated with even greater inhibitory effect when compared to control (58-62% reduction, p<0.001). Conclusions Endoglin downregulation promotes apoptosis, induces significant DNA damage through modulation of numerous DNA repair genes, and improves platinum sensitivity both in vivo and in vitro. Anti-endoglin therapy would allow dual treatment of both tumor angiogenesis and a subset of aggressive tumor cells expressing endoglin and is being actively pursued as therapy in ovarian cancer.

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“…[31][32][33][34] In contrast, the other clinicopathologic parameters were not related to MVD in the present study. 32,[46][47][48][49] Thus, evaluating the newly formed vessels may have more significant impact on associations between MVD and clinicopathological parameters of HNSCC. 35,36 Similar results were found in a study of prostate cancer, where MVD, measured by CD31 count, was not associated with any pathologic results or progression-free survival.…”

Section: Discussionmentioning

confidence: 99%

Prognostic Value of Microvessel Density and p53 Expression on the Locoregional Metastasis and Survival of the Patients With Head and Neck Squamous Cell Carcinoma

Oliveira

Gomes

Pereira

et al. 2013

Applied Immunohistochemistry &Amp; Molecular Morphology

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Cancer cells need to develop microvessels in order to grow and to establish metastatic foci. A role for the p53 protein in the regulation of the angiogenic process is suggested. This study aimed to investigate the relationship between immunohistochemical expression of microvessel density (MVD), measured by CD31 staining, and p53 protein with clinicopathologic factors, and survival in head and neck squamous cell carcinoma (n=70). Tumor angiogenesis was estimated by determining MVD in areas with the highest number of stained microvessels (hot spots). Clinicopathologic factors and immunohistochemical data were evaluated by χ statistical test and were submitted to binary logistic regression to analyze the risk of presence of lymph node metastasis. Factors that might predict survival were investigated using Cox proportional hazards tests. Differences were considered statistically significant when P<0.05. The percentage of p53-positive cells showed no association with clinicopathologic parameters and MVD. Patients with locoregional metastasis presented statistically significant higher MVD (P=0.043). Individuals presenting head and neck squamous cell carcinoma in posterior sites (P=0.022; OR=3.644) and higher MVD (P=0.039; OR=3.247) had a significant increase in risk of metastasis occurrence. Multivariate analysis showed that presence of lymph node metastasis was statistically significant for overall survival of head and neck carcinoma patients (P=0.006; OR =2.917). The present data suggest that MVD represents a promising diagnostic tool to identify individuals with increased risk for the development of metastatic disease, which is very indicative of poor prognosis.

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A Higher CD105-Assessed Microvessel Density and Worse Prognosis in Elderly Patients With Laryngeal Carcinoma

Cited by 16 publications

References 19 publications

Intratumoral vessel density as prognostic factors in head and neck squamous cell carcinoma: A meta‐analysis of literature

Intratumoral vessel density as prognostic factors in head and neck squamous cell carcinoma: A meta‐analysis of literature

Endoglin (CD105) Contributes to Platinum Resistance and Is A Target for Tumor-Specific Therapy in Epithelial Ovarian Cancer

Prognostic Value of Microvessel Density and p53 Expression on the Locoregional Metastasis and Survival of the Patients With Head and Neck Squamous Cell Carcinoma

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