A high tumour-stroma ratio (TSR) in colon tumours and its metastatic lymph nodes predicts poor cancer-free survival and chemo resistance

Abstract

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Results from the UNITED study: a multicenter study validating the prognostic effect of the tumor–stroma ratio in colon cancer

“…This study illustrates the aggressive behavior of stroma-high tumors and the potential resistance to (neo)adjuvant treatment of tumor stroma, which was also noticed in other studies by our research group. 17 , 29 , 30 , 31 …”

KIT promotes tumor stroma formation and counteracts tumor-suppressive TGFβ signaling in colorectal cancer

“…Interestingly, KIT also provides protection against tumor-suppressive TGFβ signaling, possibly by reducing the expression of SMAD2. A high stromal content in (CMS4) CRC correlates with aggressive behavior, poor survival, and a poor response to systemic therapy [ 1 , 3 , 23 , 29 – 31 ]. From a clinical perspective, there is an unmet need for effective CMS4-targeting therapies.…”

Development of Tier 2 LC-MRM-MS protein quantification methods for liquid biopsies

“…In the first step, proteotypic peptides are selected for each protein of interest through in-silico digestion. For this purpose a wide variety of software tools is online available [20] , [40] . Preferably, trypsin is used for protein digestion, since this protease is by far the most applied and developed enzyme for such purposes.…”

“…The histologic tumor-stroma ratio (TSR) quantifies the intratumoral stromal content and was found to predict poor patient prognosis in multiple solid primary tumor types [10] , [12] , [13] , [14] , [15] , [16] , [17] . In addition to patient prognosis, the TSR was reported to predict response to neoadjuvant therapy in breast and gastrointestinal cancers [18] , [19] , [20] . Lastly, the combined assessment of the TSR and histologic tumor immune cell infiltrate was recently suggested as a novel tool in predicting response to checkpoint inhibitor therapy in colon cancer [21] .…”