2013
DOI: 10.1038/srep03000
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A high-throughput three-dimensional cell migration assay for toxicity screening with mobile device-based macroscopic image analysis

Abstract: There is a growing demand for in vitro assays for toxicity screening in three-dimensional (3D) environments. In this study, 3D cell culture using magnetic levitation was used to create an assay in which cells were patterned into 3D rings that close over time. The rate of closure was determined from time-lapse images taken with a mobile device and related to drug concentration. Rings of human embryonic kidney cells (HEK293) and tracheal smooth muscle cells (SMCs) were tested with ibuprofen and sodium dodecyl su… Show more

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Cited by 86 publications
(101 citation statements)
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References 37 publications
(52 reference statements)
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“…These types of organotypic representations of tissue for drug screening are likely to become increasingly popular with the advent of patient-derived cultures [78]. These systems can be used with a more reductionist approach as well, e.g., ECM stiffness has been reported to be a powerful mediator of drug response for paclitaxel [79], ibuprofen [80], and sorafenib [81]. Ideally, the findings demonstrated here can be related to quantifiable, predictive biomarkers, informing drug treatment and monitoring.…”
Section: Predicting Drug Response For Metastatic Patientsmentioning
confidence: 99%
“…These types of organotypic representations of tissue for drug screening are likely to become increasingly popular with the advent of patient-derived cultures [78]. These systems can be used with a more reductionist approach as well, e.g., ECM stiffness has been reported to be a powerful mediator of drug response for paclitaxel [79], ibuprofen [80], and sorafenib [81]. Ideally, the findings demonstrated here can be related to quantifiable, predictive biomarkers, informing drug treatment and monitoring.…”
Section: Predicting Drug Response For Metastatic Patientsmentioning
confidence: 99%
“…Another successful example is the magnetic nanobeads-mediated spheroid formation [41][42][43][44][45][46][47][48][49] . This method (also known as 'magnetic levitation' when the cells are collected on top of the fluid in a tissue culture well rather than on its bottom 41 ), is extremely versatile.…”
Section: Cell Spheroids As Building Blocks For Bioprintingmentioning
confidence: 99%
“…monolayer scratch assay, transwell assay and EZ-TAXIScan assay) are not easily adaptable for high-throughput screening. 96-well formats can be modified and automated for microfluidic devices (Berthier et al, 2013; Guckenberger et al, 2014; Irimia and Toner, 2009; Wu et al, 2013), Boyden chambers (Hulkower and Herber, 2011; Mastyugin et al, 2004), and several image-based assays (Berthier et al, 2013; Guckenberger et al, 2014; Hattori et al, 2010; Hulkower and Herber, 2011; Kato et al, 2008; Mastyugin et al, 2004; Ponath et al, 2000; Soltaninassab et al, 2008; Timm et al, 2013; Wu et al, 2013), but they are not optimally compatible to screen ~1000 compounds. We are able screen >10,000 compounds over 5-log concentrations, for both chemotaxis and viability.…”
Section: Commentarymentioning
confidence: 99%