A High-Throughput Multiplex Genetic Detection System for
            <i>Helicobacter Pylori</i>
            Identification, Virulence and Resistance Analysis

Hu, Binjie; Zhao, Fuju; Wang, Shiwen; Olszewski, Michal A.; Bian, Haipeng; Wu, Yong; Kong, Mimi; Xu, Linfeng; Miao, Yingxin; Fang, Yi; Yang, Changqing; Hu, Zhao; Zhang, Yanmei

doi:10.2217/fmb-2016-0023

Cited by 9 publications

(14 citation statements)

References 49 publications

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“…Each biopsy specimen was homogenized by TissueLyser (Jingxin Co., Ltd, Shanghai, China) in 1 ml 0.9% sterile saline solution, and then cultured as described previously [7]. …”

Section: Methodsmentioning

confidence: 99%

“…DNA was extracted using genomic DNA extraction kit (Tiangen Biotech Co., Ltd, Beijing, China) following manufacturer’s instructions. Detailed information on DNA extraction, conventional PCR and sequencing procedures has been provided in our published work [7]. …”

Section: Methodsmentioning

confidence: 99%

“…In addition, primers for human β-globin gene were included as internal DNA loading control. The primer sequences for all these target genes and the size of the resulting amplicons were published previously [7]. The sequence of the 199-bp housekeeping gene β-globin had the primer pair as follows:…”

Section: Methodsmentioning

confidence: 99%

“…This study showed that HMGS platform was ideally optimized for these applications [7]. While highly accurate in H. pylori detection, and suitable for broad genetic screening for virulence and antibiotic resistance, the method was still culture based.…”

mentioning

confidence: 99%

“…Apart from the requirement of an additional 48-h+ culture time, it could not accurately estimate the bacterial load in biopsy material or accurately detect mixed infection with multistrains due to random selections of cultured colonies for genetic analysis. Finally, the isolation rate in culture was low (20–25%) [7], which could result in increased potential for false-negative detections. Thus, we explored possibility of bypassing the culture step and HMGS application for direct detection of H. pylori in biopsy materials (dHMGS; direct high-throughput multiple genetic detection system) with simultaneous evaluation of multiple parameters.…”

mentioning

confidence: 99%

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Direct Detection of Helicobacter Pylori in Biopsy Specimens using a High-Throughput Multiple Genetic Detection System

Zhang

Wang

et al. 2016

Future Microbiol.

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Aim We evaluated the direct high-throughput multiple genetic detection system (dHMGS) for Helicobacter pylori in gastric biopsies. Materials & methods One hundred and thirty-three specimens were concurrently analyzed by dHMGS, rapid urease test, culture and sequencing. Results dHMGS was highly sensitive and specific for H. pylori identification compared with culture and rapid urease test. The correlation coefficient of the quantitative standard curve was R2 = 0.983. A significant difference in the relative H. pylori DNA abundance was found in different gastroduodenal diseases. Concordance rates between dHMGS and sequencing for resistance mutations were 97.1, 100.0, 85.3 and 97.1%, respectively. Finally, dHMGS could efficiently distinguish mixed infection in biopsy specimens. Conclusion The dHMGS could efficiently diagnose and quantify H. pylori burden in biopsies, simultaneously screening for virulence, antibiotic resistance and presence of the multistrain infections.

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“…Each biopsy specimen was homogenized by TissueLyser (Jingxin Co., Ltd, Shanghai, China) in 1 ml 0.9% sterile saline solution, and then cultured as described previously [7]. …”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

See 3 more Smart Citations

Direct Detection of Helicobacter Pylori in Biopsy Specimens using a High-Throughput Multiple Genetic Detection System

Zhang

Wang

et al. 2016

Future Microbiol.

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Resistance mechanisms of Helicobacter pylori and its dual target precise therapy

Gong

Yuan

2018

Critical Reviews in Microbiology

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Helicobacter pylori drug resistance presents a significant challenge to the successful eradication of this pathogen. To find strategies to improve the eradication efficacy of H. pylori, it is necessary to clarify the resistance mechanisms involved. The mechanisms of H. pylori drug resistance can be investigated from two angles: the pathogen and the host. A comprehensive understanding of the molecular mechanisms of H. pylori resistance based on both pathogen and host would aid the implementation of precise therapy, or ideally "dual target precise therapy" (bacteria and host-specific target therapy). In recent years, with increased understanding of the mechanisms of H. pylori resistance, the focus of eradication has shifted from disease-specific to patient-specific treatment. The implementation of "precision medicine" has also provided a new perspective on the treatment of infectious diseases. In this article, we systematically review current research on H. pylori drug resistance from the perspective of both the pathogen and the host. We also review therapeutic strategies targeted to pathogen and host factors that are aimed at achieving precise treatment of H. pylori.

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Gastrointestinal Tract Disorders in Older Age

Dumić

Nordin

Jecmenica

et al. 2019

Canadian Journal of Gastroenterology and Hepatology

166

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Considering an increase in the life expectancy leading to a rise in the elderly population, it is important to recognize the changes that occur along the process of aging. Gastrointestinal (GI) changes in the elderly are common, and despite some GI disorders being more prevalent in the elderly, there is no GI disease that is limited to this age group. While some changes associated with aging GI system are physiologic, others are pathological and particularly more prevalent among those above age 65 years. This article reviews the most important GI disorders in the elderly that clinicians encounter on a daily basis. We highlight age-related changes of the oral cavity, esophagus, stomach, small and large bowels, and the clinical implications of these changes. We review epidemiology and pathophysiology of common diseases, especially as they relate to clinical manifestation in elderly. Details regarding management of specific disease are discussed in detail if they significantly differ from the management for younger groups or if they are associated with significant challenges due to side effects or polypharmacy. Cancers of GI tract are not included in the scope of this article.

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A High-Throughput Multiplex Genetic Detection System for Helicobacter Pylori Identification, Virulence and Resistance Analysis

Abstract: HMGS is an effective method for H. pylori identification and analysis of virulence and drug resistance.

Cited by 9 publications

References 49 publications

Direct Detection of Helicobacter Pylori in Biopsy Specimens using a High-Throughput Multiple Genetic Detection System

Direct Detection of Helicobacter Pylori in Biopsy Specimens using a High-Throughput Multiple Genetic Detection System

Resistance mechanisms of Helicobacter pylori and its dual target precise therapy

Gastrointestinal Tract Disorders in Older Age

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