A high‐throughput model for fat graft assessment

Medina, Miguel Á.; Nguyen, John; McCormack, Michael M.; Randolph, Mark A.; Austen, William G.

doi:10.1002/lsm.20874

Cited by 8 publications

(4 citation statements)

References 22 publications

(21 reference statements)

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“…This team from the Massachusetts General Hospital showed that grafts treated with P188 exhibited a 50 percent decrease in apoptosis compared to the saline-treated controls and achieved a remarkable 72 percent weight-based survival after 6 weeks. These results are confirmed by another study ( 80 ), assessing DNA contents using PicoGreen analyses, which showed higher fat survivability with P188. These findings led to the development of fat-processing systems (Vitality, SIENTRA, Irvine, CA, USA), with ongoing multicenter studies to clinically confirm these excellent results.…”

Section: Literature Review Of Tissue- and Bio-engineering Approaches ...supporting

confidence: 76%

Tissue engineering strategies for breast reconstruction: a literature review of current advances and future directions

Berkane,

Oubari,

van Dieren

et al. 2024

Ann Transl Med

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Background and Objective Mastectomy is a primary treatment for breast cancer patients, and both autologous and implant-based reconstructive techniques have shown excellent results. In recent years, advancements in bioengineering have led to a proliferation of innovative approaches to breast reconstruction. This article comprehensively explores the promising perspectives offered by bioengineering and tissue engineering in the field of breast reconstruction. Methods A literature review was conducted between April and June 2023 on PubMed and Google Scholar Databases. All English and French articles related to bioengineering applied to the field of breast reconstruction were included. We used the Evidence-Based Veterinary Medicine Association (EBVM) Toolkit 14 checklist for narrative reviews as a quality assurance measure and the Scale for the Assessment of Narrative Review Articles (SANRA) tool to self-assess our methodology. Key Content and Findings Over 130 references related to breast bioengineering were included. The analysis revealed four key applications: enhancing the quality of the skin envelope, improving the viability of fat grafting, creating breast shape and volume via bio-printing, and optimizing nipple reconstruction through engineering techniques. The primary identified approaches revolved around establishing structural support and enhancing cellular viability. Structural techniques predominantly involved the implementation of 3D printed, decellularized, or biocompatible material scaffolds. Meanwhile, promoting cellular content trophicity primarily focused on harnessing the regenerative potential of adipose-derived stem cells (ADSCs) and increasing the tissue’s survivability and cell trophicity. Conclusions Tissue and bioengineering hold immense promise in the field of breast reconstruction, offering a diverse array of approaches. By combining existing techniques with novel advancements, they have the potential to significantly enhance the therapeutic options available to plastic and reconstructive surgeons.

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Section: Literature Review Of Tissue- and Bio-engineering Approaches ...supporting

confidence: 76%

Tissue engineering strategies for breast reconstruction: a literature review of current advances and future directions

Berkane,

Oubari,

van Dieren

et al. 2024

Ann Transl Med

View full text Add to dashboard Cite

show abstract

“…No changes are seen across all other endpoints measured during this initial 24-hour period. 9 When grafts were analyzed at 6 weeks for metabolic activity, P188-treated grafts demonstrated almost 3-fold higher activity levels (Figs. 4 and 5).…”

Section: Discussionmentioning

confidence: 98%

“…Subsequent graft loss results from removal of dead cellular debris following revascularization. 9 Thus far, efforts to blunt the initial ischemic insult and protect cells until sufficient vascularity can be established have led to modest results.…”

mentioning

confidence: 99%

“…We have previously shown that decreasing apoptotic cell death results in a greater persistence of fat grafts over time. 9 It is known that the nonionic triblock copolymer classes of surfactants (poloxamers) are particularly effective for (1) repairing damaged membranes and (2) improving the viability and likelihood of survival of damaged cells. 10 -13 Damaged cell membranes expose a portion of the central lipid layer, facilitating binding of a hydrophobic moiety to the exposed hydrophobic section.…”

mentioning

confidence: 99%

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