A high throughput method to investigate nanoparticle entrapment efficiencies in biofilms

Devlin, Henry B.; Hiebner, Dishon; Barros, Caio; Fulaz, Stephanie; Quinn, Laura; Vitale, Stefania; Casey, Eoin

doi:10.1016/j.colsurfb.2020.111123

Cited by 16 publications

(19 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This effect is a clear demonstration of the Derjaguin-Landau-Verwey-Overbeek (DLVO) theory, as the repulsive electrostatic energy barrier that holds the colloid in suspension is overcome by the attractive van der Waals potential, leading to aggregation. This observation is in line with previously reported data from our group, in which confocal images of P. fluorescens interfacial biofilms exposed to amine-NPs revealed a high degree of aggregation onto the top layers of the biofilm [29]. Also, the positively charged aromatic-NPs does not undergo this charge inversion with increasing concentrations of EPS, suggesting that EPS-NPs interactions are not entirely explained by the DLVO theory.…”

Section: Plos Onesupporting

confidence: 92%

“…This hypothesis is confirmed by looking at the stability and non-significant increase in the size of carboxylate-NPs in the presence of EPS (Fig 2), whereas the bare NPs shows an increase of about 40 nm in diameter, which is clearly more than a protein monolayer and in line with the presence of mainly negatively charged proteins. These results also agree with the fact that carboxylate-NPs has a higher uptake into P. fluorescens biofilms when compared to bare NPs [29]; the interaction with positively charged proteins might have a role in the differential penetration of NPs into the matrix. A qualitative analysis was also done to compare differences in expressions of proteins based on the heat map generated.…”

Section: Plos Onesupporting

confidence: 88%

“…This observation is in line with previously reported data from our group, in which confocal images of P . fluorescens interfacial biofilms exposed to amine-NPs revealed a high degree of aggregation onto the top layers of the biofilm [ 29 ]. Also, the positively charged aromatic-NPs does not undergo this charge inversion with increasing concentrations of EPS, suggesting that EPS-NPs interactions are not entirely explained by the DLVO theory.…”

Section: Resultsmentioning

confidence: 99%

“…These results also agree with the fact that carboxylate-NPs has a higher uptake into P . fluorescens biofilms when compared to bare NPs [ 29 ]; the interaction with positively charged proteins might have a role in the differential penetration of NPs into the matrix. A qualitative analysis was also done to compare differences in expressions of proteins based on the heat map generated.…”

Section: Resultsmentioning

confidence: 99%

See 3 more Smart Citations

Interactions between functionalised silica nanoparticles and Pseudomonas fluorescens biofilm matrix: A focus on the protein corona

et al. 2020

Self Cite

View full text Add to dashboard Cite

Biofilms are microbial communities embedded in an extracellular polymeric matrix and display an enhanced tolerance to the action of antimicrobials. The emergence of novel functionalised nanoparticles is considered a promising avenue for the development of biofilmspecific antimicrobial technologies. However, there is a gap in the understanding of interactions between nanoparticles and the biofilm matrix. Particularly, questions are raised on how nanoparticle charge and surface groups play a role in aggregation when in contact with biofilm components. Herein we present the synthesis of four types of silica nanoparticles and undertake an analysis of their interactions with Pseudomonas fluorescens biofilm matrix. The effect of the biofilm matrix components on the charge and aggregation of the nanoparticles was assessed. Additionally, the study focused on the role of matrix proteins, with the in-depth characterisation of the protein corona of each nanoparticle by Liquid Chromatography with Tandem Mass Spectrometry experiments. The protein corona composition is dependent on the nanoparticle type; non-functionalised nanoparticles show less protein selectivity, whereas carboxylate-functionalised nanoparticles prefer proteins with a higher isoelectric point. These outcomes provide insights into the field of biofilm-nanoparticle interactions that can be valuable for the design of new nano-based targeting systems in future anti-biofilm applications.

show abstract

Section: Plos Onesupporting

confidence: 92%

Section: Plos Onesupporting

confidence: 88%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

See 2 more Smart Citations

Interactions between functionalised silica nanoparticles and Pseudomonas fluorescens biofilm matrix: A focus on the protein corona

et al. 2020

Self Cite

View full text Add to dashboard Cite

show abstract

“…The pegs were then transferred into a sterile black 96-well plate, and each magnet was secured into position against the upper wall of the well using a small magnet (5mm x 5mm, nickel-plated neodymium magnet, Supermagnete). The biofilms were then exposed to various nanoparticles (MSN-B, MSN-D, MSN-C and MSN-A) at a concentration of 1 mg mL −1 for 3 h with fluorescent intensity readings taken every 10 mins to assess entrapment in a method similar to Fulaz et al, 2019 14 and Devlin et al 46 Briefly, the reduction in fluorescent intensity from MSNs exposed to biofilm coated pegs compared to that of a control was used to calculate the entrapment of MSNs. Assays were carried out in triplicate, with a minimum of 12 individual replicates per run.…”

Section: Msn Entrapmentmentioning

confidence: 99%

<p>Tailoring Nanoparticle-Biofilm Interactions to Increase the Efficacy of Antimicrobial Agents Against <em>Staphylococcus aureus</em></p>

Fulaz¹,

Devlin²,

Vitale³

et al. 2020

IJN

Self Cite

View full text Add to dashboard Cite

Background: Considering the timeline required for the development of novel antimicrobial drugs, increased attention should be given to repurposing old drugs and improving antimicrobial efficacy, particularly for chronic infections associated with biofilms. Methicillinsusceptible Staphylococcus aureus (MSSA) and methicillin-resistant S. aureus (MRSA) are common causes of biofilm-associated infections but produce different biofilm matrices. MSSA biofilm cells are typically embedded in an extracellular polysaccharide matrix, whereas MRSA biofilms comprise predominantly of surface proteins and extracellular DNA (eDNA). Nanoparticles (NPs) have the potential to enhance the delivery of antimicrobial agents into biofilms. However, the mechanisms which influence the interactions between NPs and the biofilm matrix are not yet fully understood. Methods: To investigate the influence of NPs surface chemistry on vancomycin (VAN) encapsulation and NP entrapment in MRSA and MSSA biofilms, mesoporous silica nanoparticles (MSNs) with different surface functionalization (bare-B, amine-D, carboxyl-C, aromatic-A) were synthesised using an adapted Stöber method. The antibacterial efficacy of VAN-loaded MSNs was assessed against MRSA and MSSA biofilms. Results: The two negatively charged MSNs (MSN-B and MSN-C) showed a higher VAN loading in comparison to the positively charged MSNs (MSN-D and MSN-A). Cellular binding with MSN suspensions (0.25 mg mL −1) correlated with the reduced viability of both MSSA and MRSA biofilm cells. This allowed the administration of low MSNs concentrations while maintaining a high local concentration of the antibiotic surrounding the bacterial cells. Conclusion: Our data suggest that by tailoring the surface functionalization of MSNs, enhanced bacterial cell targeting can be achieved, leading to a novel treatment strategy for biofilm infections.

show abstract

Polyethylenimine‐grafted mesoporous silica nanocarriers markedly enhance the bactericidal effect of curcumin against Staphylococcus aureus biofilm

2022

View full text Add to dashboard Cite

The recalcitrant nature of biofilms makes biofilm‐associated infections difficult to treat in modern medicine. Biofilms have a high vulnerability to antibiotics and a limited repertoire of antibiotics could act on matured biofilms. This issue has resulted in a gradual paradigm shift in drug discovery and therapy, with anti‐biofilm compounds being sought alongside new drug carriers. A potential solution to biofilm‐associated infections is to employ antibiofilm treatments, which can attack biofilms from many fronts. Nanocarriers are promising in this regard because they can be entrapped within biofilm matrix, target biofilm matrix, and provide local drug delivery to inhibit biofilm formation. In this study, curcumin as an herbal extract was loaded onto hyperbranched polyethylenimine‐grafted mesoporous silica nanoparticles (F‐MSN‐PEI/Cur) and antibiofilm investigations were performed. The F‐MSN‐PEI/Cur design has the potential to repurpose curcumin as an antibiofilm agent by increasing its solubility and lowering the required doses for the destruction of matured biofilms as well as suppressing biofilm development. Using imaging and spectroscopic techniques, we assessed the interaction of F‐MSN‐PEI/Cur with Staphylococcus aureus bacterial cells and determined the impact of F‐MSN‐PEI/Cur on eradicating matured biofilms and suppressing biofilm development. The F‐MSN‐PEI/Cur design is highly cytocompatible, as observed by the cytotoxicity screening investigations on L929 mouse fibroblast cell line. Our findings show that F‐MSN‐PEI/Cur design reduces the bacterial cell viability, inhibits biofilm formation, and induces biofilm eradication, which is attributed to F‐MSN‐PEI/Cur design having the potential to repurpose the antibiofilm activity of curcumin‐herbal extract.

show abstract

A high throughput method to investigate nanoparticle entrapment efficiencies in biofilms

Cited by 16 publications

References 40 publications

Interactions between functionalised silica nanoparticles and Pseudomonas fluorescens biofilm matrix: A focus on the protein corona

Interactions between functionalised silica nanoparticles and Pseudomonas fluorescens biofilm matrix: A focus on the protein corona

<p>Tailoring Nanoparticle-Biofilm Interactions to Increase the Efficacy of Antimicrobial Agents Against <em>Staphylococcus aureus</em></p>

Polyethylenimine‐grafted mesoporous silica nanocarriers markedly enhance the bactericidal effect of curcumin against Staphylococcus aureus biofilm

Contact Info

Product

Resources

About