A high-throughput method for measuring growth and loss rates in microalgal cultures

Skjelbred, Birger; Edvardsen, Bente; Andersen, Tom

doi:10.1007/s10811-012-9819-z

Cited by 17 publications

(13 citation statements)

References 50 publications

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“…Assay for estimating growth and loss rates. The main experiments were carried out essentially as described by Skjelbred et al (2012) in 96-well culture plates with white walls and optical bottoms (Nunc AS). Each strain was exposed to a 3-way factorial design with six temperatures (4°C-21°C), five salinities (15,20,25,30,35), and eight irradiance levels (21.7 -96.7 lmol Á photons Á m À2 Á s À1 ; 14:10 L:D).…”

Section: Methodsmentioning

confidence: 99%

Environmental Optima for Seven Strains of Pseudochattonella (Dictyochophyceae, Heterokonta)

Skjelbred

Edvardsen

Andersen

2012

Journal of Phycology

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The ichthyotoxic flagellate Pseudochattonella has formed recurrent blooms in the North Sea, Skagerrak and Kattegat since 1998. Five strains of Pseudochattonella farcimen and two strains of P. verruculosa were examined in an assay comparing the light response of specific growth rates over a range of temperatures and salinities to get further knowledge on the autecology of members of this genus. Temperature optima were lower in P. farcimen (9°C-15°C) than in P. verruculosa (12°C-20°C). P. farcimen also showed a somewhat lower salinity optimum (18-26) than P. verruculosa (20-32). All strains showed light-dependent growth responses reaching saturation between 18 and 52 μmol · photons · m(-2) · s(-1) at optimal temperature and salinity conditions. Compensation point estimates ranged from 4.2 to 15 μmol · photons · m(-2) · s(-1) . Loss rates increased with temperature and were lowest at salinities close to optimal growth conditions. Blooms of P. farcimen have been recorded in nature under conditions more similar to those minimizing loss rates rather than those maximizing growth rates in our culture study.

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Section: Methodsmentioning

confidence: 99%

Environmental Optima for Seven Strains of Pseudochattonella (Dictyochophyceae, Heterokonta)

Skjelbred

Edvardsen

Andersen

2012

Journal of Phycology

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“…Therefore, we developed a specific experimental device based on microplates. Similar tools have been previously used to assess growth for microalgae [40]. However, in this study, we had to face specific constraints, including the presence of bacteria that can affect optical measurements for microalgae concentration and bacterial cross-contamination between wells.…”

Section: High-throughput Selection Of Growth-promoting Bacteriamentioning

confidence: 99%

Screening and selection of growth-promoting bacteria for Dunaliella cultures

et al. 2013

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Previous studies have demonstrated that bacteria influence microalgal metabolism, suggesting that the selection and characterization of growth-promoting bacteria should offer a new strategy for improving industrial algal cultivation. In the present study, 48 cultivable bacteria were isolated from marine microalgae species and identified using 16S rRNA phylogenetic analysis. The recovered bacteria were found to be members of the α-and γ-Proteobacteria, Cytophaga-Flavobacterium-Bacteroides (CFB) and gram-positive monophyletic clusters. To address the effect of these bacteria on the growth of Dunaliella sp. individually, an experimental high-throughput tool was developed to simultaneously compare replicated associations. A two-step approach was used to monitor growth rate and biomass accumulation of Dunaliella sp. in mixed culture with bacteria, which proved the highthroughput device to be an efficient tool for the selection of growth-promoting bacteria. Depending on the bacterial strain involved, inhibitory effects were recorded for maximal microalgal growth rate, whereas inhibitory and stimulating effects were registered on microalgal biomass accumulation and nitrogen incorporation. Organic nitrogen remineralization by Alteromonas sp. SY007 and Muricauda sp. SY244 is discussed to explain the higher biomass and ammonium incorporation of Dunaliella sp. obtained under nitrogen-limited conditions. These bacteria could be considered as helpers for N accumulation in Dunaliella sp. cells.

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“…Experiments were conducted on altogether 4 white 96-well plates with clear, optical bottoms (Perkin-Elmer Optiplate™ 96). White wells were chosen to reduce light noise from adjacent wells when reading the fluorescence, and to reduce absorption and attenuation of light (Skjelbred et al 2012), while optical bottoms ensured correct readings without reflection. Each treatment in the experiment was conducted with 2 replicates on each plate, and on 2 identical plates, creating a total of 4 replicates.…”

Section: Experimental Designmentioning

confidence: 99%

Growth responses of the nuisance algae Gonyostomum semen (Raphidophyceae) to DOC and associated alterations of light quality and quantity

Hagman¹,

Skjelbred²,

Thrane³

et al. 2019

Aquat. Microb. Ecol.

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A high-throughput method for measuring growth and loss rates in microalgal cultures

Cited by 17 publications

References 50 publications

Environmental Optima for Seven Strains of Pseudochattonella (Dictyochophyceae, Heterokonta)

Environmental Optima for Seven Strains of Pseudochattonella (Dictyochophyceae, Heterokonta)

Screening and selection of growth-promoting bacteria for Dunaliella cultures

Growth responses of the nuisance algae Gonyostomum semen (Raphidophyceae) to DOC and associated alterations of light quality and quantity

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