A high throughput method for egg size measurement in Drosophila

Barghi, Neda; Ramirez-Lanzas, Claudia

doi:10.1038/s41598-023-30472-8

Cited by 2 publications

(2 citation statements)

References 28 publications

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“…Once oviposition occurred on soybean leaves, a single egg cluster (non-overlapping) was separated and counted. Egg viability was determined based on the shape, size, and color of eggs using a microscope (LEICA EZ4D, Wetzlar, Germany) [ 55 ]. The selected egg clusters attached to the soybean leaves were placed into a Petri dish (9.8 cm dia.…”

Section: Methodsmentioning

confidence: 99%

Temperature and Host Plant Impacts on the Development of Spodoptera litura (Fabricius) (Lepidoptera: Noctuidae): Linear and Nonlinear Modeling

Maharjan

Hong

Ahn

et al. 2023

Insects

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The tobacco cutworm, Spodoptera litura (Fabricius) (Lepidoptera: Noctuidae), is one of the most serious pests in field crops, vegetables, and ornamentals. Temperatures (15, 20, 25, 27, 30, 35, and 40 °C), host plants (soybean (Glycine max (L.)), maize (Zea mays L.), groundnut (Arachis hypogaea L.) and azuki bean (Vigna angularis (Willd.) Ohwi & H. Ohashi,), and the artificial diet-dependent developmental parameters and survival of S. litura were examined in this study. Stage-specific parameters such as threshold development temperature (LDT) and thermal constant (K) (Degree day (DD)) were determined by linear and nonlinear models (Sharpe–Schoolfield–Ikemoto), respectively. The total developmental time (egg–adult) decreased with increasing temperature on host plants and with an artificial diet. The total immature developmental time varied from 106.29, 107.57, 130.40, 111.82, and 103.66 days at 15 °C to 22.47, 21.25, 25.31, 18.30, and 22.50 days at 35 °C on soybean, maize, groundnut, azuki bean, and artificial diet, respectively. The LDT for the total immature completion was 7.50, 9.48, 11.44, 12.32, and 7.95 °C on soybean, maize, groundnut, azuki bean, and artificial diet, respectively. The K for the total immature completion was 587.88, 536.84, 517.45, 419.44, and 586.95 DD on soybean, maize, groundnut, azuki bean, and artificial diet, respectively. Temperature and host plant interaction also influenced the longevity and survival of adults. The findings of this study can be used to predict the number of generations, spring emergence, and population dynamics of S. litura. The nutrient content analysis of the host plants is discussed in terms of the developmental patterns of S. litura.

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Section: Methodsmentioning

confidence: 99%

Temperature and Host Plant Impacts on the Development of Spodoptera litura (Fabricius) (Lepidoptera: Noctuidae): Linear and Nonlinear Modeling

Maharjan

Hong

Ahn

et al. 2023

Insects

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“…where S-follicle size (volume), L-follicle length (corresponds to the axis of rotational symmetry), and W-follicle width (corresponds to the axis perpendicular to length) [79,80].…”

mentioning

confidence: 99%

Signal and Nutritional Effects of Mixed Diets on Reproduction of a Predatory Ladybird, Cheilomenes propinqua

Овчинников

Овчинникова

Reznik

et al. 2023

Insects

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It is known that food has a double impact on females of predatory ladybirds: qualitative signal effect (the onset of oogenesis) and quantitative nutritional effect (the increase in oogenesis intensity). We compared the patterns of these effects by feeding Cheilomenes propinqua females on mixed diets: unlimited low-quality prey (eggs of the grain moth Sitotroga cerealella) and limited high-quality prey (the green peach aphid Myzus persicae: 0, 2, 10, and 50 aphids per day). About half of the females fed only on the grain moth eggs oviposited and their fecundity was very low. Daily consumption of 2 aphids increased the proportion of egg-laying females whereas only consumption of 10 aphids increased their mean fecundity. Thus, the threshold of the signal effect was lower than that of the nutritional effect. As applied to mass rearing, we conclude that the addition of high-quality prey to low-quality food causes a substantial increase in egg production, although the economic feasibility of this method is not clear. Regarding biological control of pests by preventing colonization, we conclude that the fecundity of C. propinqua females supplied with the grain moth eggs in the absence of aphids will be low but the appearance of pests will cause a proportional increase in the mean fecundity of ladybirds.

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A high throughput method for egg size measurement in Drosophila

Cited by 2 publications

References 28 publications

Temperature and Host Plant Impacts on the Development of Spodoptera litura (Fabricius) (Lepidoptera: Noctuidae): Linear and Nonlinear Modeling

Temperature and Host Plant Impacts on the Development of Spodoptera litura (Fabricius) (Lepidoptera: Noctuidae): Linear and Nonlinear Modeling

Signal and Nutritional Effects of Mixed Diets on Reproduction of a Predatory Ladybird, Cheilomenes propinqua

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