A high-throughput LC-MS/MS method for the quantification of four immunosu- ppressants drugs in whole blood

Gong, Zhaojian; Wu, Zhonghao; Xu, Shuxin; Han, Wen-Nian; Jiang, Xiaomei; Liu, Hai-Pei; Yanli,; Wei-Hu,; Yan-Wang,

doi:10.1016/j.cca.2019.07.026

Cited by 28 publications

(19 citation statements)

References 19 publications

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“…Tacrolimus and mycophenolate mofetil are used as immunosuppressive drugs in lung‐transplanted patients, and itraconazole and voriconazole are used as antifungal drugs, which are monitored using TDM. Because tacrolimus can only be determined in whole blood samples (Gong et al, 2019; Karapirli et al, 2012), this study determined the levels of mycophenolic acid, itraconazole, and voriconazole in plasma samples. In addition, because the metabolites of itraconazole and hydroxyitraconazole can be determined using TDM (Jenkins et al, 2018), a new method for simultaneously determining the concentrations of four compounds was developed.…”

Section: Resultsmentioning

confidence: 99%

“…Blood concentrations of these drugs are measured using immunoassay, HPLC with ultraviolet detection, LC-MS, and LC-tandem mass spectrometry (LC-MS/MS) (Ashbee et al, 2014;Hamada et al, 2013;Yang & Wang, 2008). In clinical practice, along with high accuracy and high sensitivity, a simple and rapid analysis method is required, and a method for the simultaneous analysis of immunosuppressive and antifungal drugs using LC-MS/MS has been developed in recent years (Gong et al, 2019;Jenkins, Black, & Schneider, 2018;Karapirli et al, 2012;Yoon, Lee, Oh, Woo, & Lee, 2019). However, these methods can only analyze drugs belonging to the same class, such as immunosuppressive or antifungal drugs.…”

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confidence: 99%

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Simultaneous analysis of drugs administered to lung‐transplanted patients using liquid chromatography–tandem mass spectrometry for therapeutic drug monitoring

Takasaki

Hirasawa

Sato

et al. 2021

Biomedical Chromatography

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Several drugs are administered to lung‐transplanted patients, which are monitored using therapeutic drug monitoring (TDM). Therefore, we developed and validated a liquid chromatography–tandem mass spectrometry method to simultaneously analyze immunosuppressive drugs such as mycophenolic acid, antifungal drugs such as voriconazole and itraconazole, and its metabolite hydroxyitraconazole. Chromatographic separation was achieved using a C18 column and gradient flow of mobile phase comprising 20 mM aqueous ammonium formate and 20 mM ammonium formate‐methanol solution. A simple protein precipitation treatment was performed using acetonitrile/methanol and mycophenolic acid‐2H3, voriconazole‐2H3, itraconazole‐2H4, and hydroxyitraconazole‐2H4 as internal standards. The linearity ranges of mycophenolic acid, voriconazole, itraconazole, and hydroxyitraconazole were 100–20,000, 50–10,000, 5–1000, and 5–1000 ng/mL, respectively. The retention time of each target was less than 2 min. The relative errors in intra‐ and inter‐day were within ±7.6%, the coefficient of variation was 8.9% or less for quality control low, medium, and high, and it was 15.8% or less for lower limit of quantitation. Moreover, the patient samples were successfully quantified, and they were within the linear range of measurements. Therefore, our new method may be useful for TDM in lung‐transplanted patients.

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Section: Resultsmentioning

confidence: 99%

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confidence: 99%

Simultaneous analysis of drugs administered to lung‐transplanted patients using liquid chromatography–tandem mass spectrometry for therapeutic drug monitoring

Takasaki

Hirasawa

Sato

et al. 2021

Biomedical Chromatography

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“…The traditional analytical methods used to measure sirolimus mainly include high-performance liquid chromatography (HPLC) 13,14 and liquid chromatography-mass spectrometry (LC-MS). 11,15,16 Most clinical laboratories use LC-MS/MS as the first choice for drug detection, due to its high sensitivity and specificity. 17 However, the disadvantages of these methods are that they are cumbersome, are time-consuming, require highly skilled operators, and are costly to run.…”

Section: Introductionmentioning

confidence: 99%

“…However, sirolimus has a long half-life, a narrow therapeutic window, and large individual variations in the pharmacokinetics 8 and therefore, its improper use may cause adverse reactions, such as immune rejection, thrombocytopenia, leucopenia, or hyperlipidemia. 9,10 Immunosuppressive drugs in the body are usually measured once or twice a day before the patient is given a new dosage 11 and it is, therefore, important to monitor their blood levels for the duration of any treatment. In addition, a rapid and efficient monitoring method for sirolimus should allow its quantification in human whole blood, because it is mainly distributed in erythrocytes.…”

Section: Introductionmentioning

confidence: 99%

A gold-based immunochromatographic strip for the detection of sirolimus in human whole blood

Jiang

Zeng

et al. 2022

Analyst

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“…A number of detection methods, including ultraviolet (UV), fluorescence (FL), mass spectrometry (MS), and electrochemical detection, have been employed for the determination of S1P in blood samples. Liquid chromatography‐tandem mass pectrometry (LC‐MS/MS) is now well accepted technology because of greater sensitivity, quantitative and specificity . So far, LC‐MS/MS methods have been developed only for the determination of the S1P content in plasma .…”

Section: Introductionmentioning

confidence: 99%

Validated LC‐MS/MS method of Sphingosine 1‐phosphate quantification in human serum for evaluation of response to radiotherapy in lung cancer

Tang

Chen

et al. 2020

Thoracic Cancer

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Background Sphingosine 1‐phosphate (S1P), a bioactive lipid, has been shown to mediate cancer processes. Therefore, accurate qualitative and quantitative determination is essential. The current assay method is still cumbersome to be of practical use worldwide and the aim of this study was therefore to develop a fast, accurate, precise and efficient LC‐MS/MS method for targeted analyses of S1P in serum samples. Methods Liquid chromatography‐tandem mass spectrometry (LC‐MS/MS) is an established method used for monitoring and analyzing S1P levels in serum. We determined the level of serum S1P in 256 patients with lung cancer and 36 healthy donors, and used Spearman';s rank correlation analysis to evaluate the difference in serum S1P levels between radiotherapy and nonradiotherapy patients. Results Standard curves were linear over ranges of 25–600 ng/mL for S1P with correlation coefficient (r2) greater than 0.9996. The lower limit of quantifications (LLOQs) was 25 ng/mL. The intra‐ and interbatch precisions and accuracy was less than 10% for S1P. The recoveries of the method were found to be 80%–98%. Serum S1P levels in healthy donors were different from those in patients (P < 0.001). Of 256 lung cancer patients, 124 (48.4%) received radiotherapy and were identified to have concomitant low serum S1P levels (222.13 ± 48.63), whereas 132 (51.6%) who had not received radiotherapy were identified to have high levels (315.16 ± 51.06). The serum S1P levels were therefore associated with radiotherapy (Spearman's Rho = −0.653, P < 0.001). Conclusions Our results indicated that this new LC‐MS/MS method is rapid, sensitive, specific and reliable for the quantification of S1P levels in serum samples. The level of S1P in serum samples of patients with lung cancer who received radiotherapy was significantly lower than that in patients who did not receive radiotherapy. Key points An improved method was established to quantify S1P levels in human serum by LC‐MS/MS, which enabled the change in serum S1P levels in lung cancer patients to be monitored, in combination with radiotherapy, and their clinical significance to be analyzed.

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A high-throughput LC-MS/MS method for the quantification of four immunosu- ppressants drugs in whole blood

Cited by 28 publications

References 19 publications

Simultaneous analysis of drugs administered to lung‐transplanted patients using liquid chromatography–tandem mass spectrometry for therapeutic drug monitoring

Simultaneous analysis of drugs administered to lung‐transplanted patients using liquid chromatography–tandem mass spectrometry for therapeutic drug monitoring

A gold-based immunochromatographic strip for the detection of sirolimus in human whole blood

Validated LC‐MS/MS method of Sphingosine 1‐phosphate quantification in human serum for evaluation of response to radiotherapy in lung cancer

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