A high throughput gold nanoparticles chemiluminescence detection of opioid receptor antagonist naloxone hydrochloride

Abstract

BackgroundThe opioid antagonist agent naloxone hydrochloride (NLX) is a drug that has high affinity for opiate receptors but do not activate these receptors. Owing to the role of this drug to block the effects of exogenous administered opioids and endogenous released endorphians we can deduce the importance of developing sensitive analytical methods for detection of such drug. In the present study gold nanoparticles (AuNPs) was employed for enhancing the chemiluminescence (CL) signals arising from luminol-ferricyanide reaction in the presence of naloxone hydrochloride using sequential injection chemiluminescence analysis (SIA).MethodIn the present study gold nanoparticles (AuNPs) was employed for enhancing the chemiluminescence (CL) signals arising from luminol-ferricyanide reaction in the presence of naloxone hydrochloride using sequential injection chemiluminescence analysis (SIA).ResultsThe developed method was examined under optimum experimental conditions and the obtained results revealed a linear relationship between the relative CL intensity and the investigated drug at a concentration range of 1.0×10−9-1.0×10−2 mol L−1, (r = 0.9993, n=9) with detection and quantification limits of 1.6×10−11 and 1.0×10−9 mol L−1, respectively. The relative standard deviation was 0.9%.ConclusionThe proposed method was employed for the determination of the investigated drug in bulk powder, its pharmaceutical dosage forms and biological fluids. The interference of some metals and amino acids on the CL intensity was investigated. Also the interference of some related pharmacological action drugs was tested. The obtained results of the developed method were statistically treated and compared with those obtained from other reported methods.Graphical AbstractUtility of gold nanparticles in luminol-potassium ferricyanide chemiluminescence system for determination of naloxone hydrochloride.

Molecularly imprinted electrochemical sensor prepared on a screen printed carbon electrode for naloxone detection

“…Opioid dependence is a neurobehavioral syndrome, affecting millions of people worldwide, characterized by the repeated, compulsive seeking and use of an opioid despite adverse social, psychological, and/or physical consequences [1]. Naloxone (NLX), is a derivative of morphine and a specific opioid antagonist at mu, kappa, and delta opioid receptors [1,2] witch as high affinity for opiate receptors without activate then [3]. It reverses the action of many narcotic drugs, including heroin, and thus can be used in the treatment of overdoses.…”

Sodium alginate and naloxone loaded macrophage-derived nanovesicles for the treatment of spinal cord injury

“…Naloxone (NAL) is an opioid receptor antagonist with no intrinsic activity. However, it can competitively antagonize various types of opioid receptors with a strong affinity for µ receptors [26] . It is frequently used in the differential diagnosis of opioid overdose-induced respiratory depression, coma, and opioid addiction.…”

Selective synthesis of Fe3O4Au x Ag y nanomaterials and their potential applications in catalysis and nanomedicine

“…Indeed, these nanocomposites are useful in promoting light induced electron-transfer reactions and can be used as a powerful material for charge separation. In addition, individually, Ag NPs have a high antibacterial activity [ 64 ], Au NPs are optical active [ 65 ] while Fe 3 O 4 NPs are supermagnetic [ 9 ]. All these properties make Fe 3 O 4 Au x Ag y NPs convenient to be used in magnetic separation and in catalytic degradation of pollutants.…”