A high throughput CRISPR perturbation screen identifies epigenetic regulators impacting primordial germ cell development
Liangdao Li,
Xinbao Ding,
Alex P. Sheft
et al.
Abstract:Certain environmental factors can impact fertility and reproductive parameters such as the number and quality of sperm and eggs. One possible mechanism is the perturbation of epigenetic landscapes in the germline. To explore this possibility, we conducted a CRISPRi screen of epigenetic-related genes to identify those that specifically perturb the differentiation of embryonic stem cells (ESCs) into primordial germ cell-like cells (PGCLCs), exploiting a highly scalable cytokine-free platform. Of the 701 genes sc… Show more
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