A high-throughput and selective method for the measurement of surface areas of silver nanoparticles

Agustin, Yuana Elly; Tsai, Shen‐Long

doi:10.1039/c4an01951b

Cited by 3 publications

(2 citation statements)

References 20 publications

(20 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To display a fusion protein containing a yellow fluorescent protein (YFP) and an Src homology 3 (SH3) domain [ 35 ] on the surface of P. putida HmfH‐CscA, pVLT33‐INP‐YFP‐SH3d that has the YFP‐SH3 domain fusion in frame with the surface anchor, ice‐nucleation protein (INP), was constructed as follows. The genes of INP, YFP, and SH3 domain were duplicated from pINPOPH, [ 36 ] pET24(a)‐pd‐YFP [ 37 ] and pET‐Plac‐EC‐SH3d (an in‐house plasmid), respectively, and then assembled by overlap PCR amplification. The PCR product was further digested and inserted into pVLT33s (an in‐house plasmid, in which the kanamycin selection marker of pVLT33 was replaced with spectinomycin).…”

Section: Methodsmentioning

confidence: 99%

Biotransformation of 5‐Hydroxymethylfurfural to 2,5‐Furandicarboxylic Acid by a Syntrophic Consortium of Engineered Synechococcus elongatus and Pseudomonas putida

Lin

Wen

Shen

et al. 2020

Biotechnology Journal

View full text Add to dashboard Cite

2,5-furandicarboxylic acid (FDCA) is one of the top platform chemicals that can be produced from biomass feedstock. To make the cost of industrial FDCA production compatible with plastics made from fossils, the price of substrates and process complexity should be reduced. The aim of this research is to create a CO 2 -driven syntrophic consortium for the catalytic conversion of renewable biomass-derived 5-hydroxymethylfurfural (HMF) to FDCA. Sucrose produced from carbon fixation by the engineered Synechococcus elongatus serves as the sole carbon source for the engineered Pseudomonas putida to catalyze the reaction of HMF to FDCA. The yield of FDCA by the consortium reaches around 70% while the conversion of HMF is close to 100%. With further surface engineering to clump the two strains, the FDCA yield is elevated to almost 100% via the specific association between an Src homology 3 (SH3) domain and its ligand. The syntrophic consortium successfully demonstrates its green and cost-effective characteristics for the conversion of CO 2 and biomass into platform chemicals.

show abstract

Section: Methodsmentioning

confidence: 99%

Biotransformation of 5‐Hydroxymethylfurfural to 2,5‐Furandicarboxylic Acid by a Syntrophic Consortium of Engineered Synechococcus elongatus and Pseudomonas putida

Lin

Wen

Shen

et al. 2020

Biotechnology Journal

View full text Add to dashboard Cite

show abstract

“…BET involves exposing solid materials to nitrogen gas under a variety of conditions and assessing the mass uptake. Furthermore, Augustin et al proposed a method to improve indirect SA measurement of silver NPs by specific biomolecules' affinity coordination [2]. The improved technique, however, is highly sensitive to the chemical composition of the NPs.…”

Section: Introductionmentioning

confidence: 99%

Direct surface area measurement from digital images via brightness histogram method

Sendova¹,

Mancini²

2020

Meas. Sci. Technol.

View full text Add to dashboard Cite

A direct, shape-and scale-independent digital image measurement method of the surface area (SA) of individual objects is proposed. The algorithms presented herein utilize the brightness histogram of 8-bit greyscale images, and thus are referred to as a brightness histogram surface area measurement algorithm (BHSAMA). The proposed SA measurement technique allows the uncertainty of the single measurement reading to be evaluated by traditional error propagation. Furthermore, the numerical value of the propagated uncertainty reflects the pixel brightness gradient of the edge pixels. This fact alone presents a significant advantage to the current methods utilizing image segmentation and/or edge detection whose measurement uncertainties cannot be quantitatively analysed. The proposed method does not involve any shape-related approximations. Five examples illustrating the method are discussed. For method verification purposes, a series of digital simulations using a control sample of predetermined size is undertaken. The accuracy of the single measured SA reading is between 0.5% and 1.5%. The uncertainty of the measured SA is evaluated and discussed further as a function of two types of simulated blurred edge region. The two presented BHSAMA techniques can have a wide range of applicability, from nanoparticles to cell biology to aerial imagery.

show abstract

Exploiting Plug-And-Play Electrochemistry for Drug Discovery

Gao

Teng

2016

Future Med. Chem.

View full text Add to dashboard Cite

Electrochemistry has emerged as a powerful analytical technique for chemical analysis of living cells, biologically active molecules and metabolites. Electrochemical biosensor, microfluidics and mass spectrometry are the most frequently used methods for electrochemical detection and monitory, which comprise a collection of extremely useful measurement tools for various fields of biology and medicine. Most recently, electrochemistry has been shown to be coupled with nanotechnology and genetic engineering to generate new enabling technologies, providing rapid, selective, and sensitive detection and diagnosis platforms. The primary focus of this review is to highlight the utility of electrochemical strategies and their conjunction with other approaches for drug metabolism and discovery. Current challenges and possible future developments and applications of electrochemistry in drug studies are also discussed.

show abstract

A high-throughput and selective method for the measurement of surface areas of silver nanoparticles

Cited by 3 publications

References 20 publications

Biotransformation of 5‐Hydroxymethylfurfural to 2,5‐Furandicarboxylic Acid by a Syntrophic Consortium of Engineered Synechococcus elongatus and Pseudomonas putida

Biotransformation of 5‐Hydroxymethylfurfural to 2,5‐Furandicarboxylic Acid by a Syntrophic Consortium of Engineered Synechococcus elongatus and Pseudomonas putida

Direct surface area measurement from digital images via brightness histogram method

Exploiting Plug-And-Play Electrochemistry for Drug Discovery

Contact Info

Product

Resources

About