2015
DOI: 10.1039/c4an01951b
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A high-throughput and selective method for the measurement of surface areas of silver nanoparticles

Abstract: A high-throughput and selective method based on biomolecule affinity coordination was employed for measuring nanoparticle surface area in solutions. In this design, silver binding peptides (AgBPs) are immobilized on bacterial cellulose via fusion with cellulose binding domains to capture silver nanoparticles whereas green fluorescent proteins are fused with AgBPs as reporters for surface area quantification.

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Cited by 3 publications
(2 citation statements)
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“…To display a fusion protein containing a yellow fluorescent protein (YFP) and an Src homology 3 (SH3) domain [ 35 ] on the surface of P. putida HmfH‐CscA, pVLT33‐INP‐YFP‐SH3d that has the YFP‐SH3 domain fusion in frame with the surface anchor, ice‐nucleation protein (INP), was constructed as follows. The genes of INP, YFP, and SH3 domain were duplicated from pINPOPH, [ 36 ] pET24(a)‐pd‐YFP [ 37 ] and pET‐Plac‐EC‐SH3d (an in‐house plasmid), respectively, and then assembled by overlap PCR amplification. The PCR product was further digested and inserted into pVLT33s (an in‐house plasmid, in which the kanamycin selection marker of pVLT33 was replaced with spectinomycin).…”
Section: Methodsmentioning
confidence: 99%
“…To display a fusion protein containing a yellow fluorescent protein (YFP) and an Src homology 3 (SH3) domain [ 35 ] on the surface of P. putida HmfH‐CscA, pVLT33‐INP‐YFP‐SH3d that has the YFP‐SH3 domain fusion in frame with the surface anchor, ice‐nucleation protein (INP), was constructed as follows. The genes of INP, YFP, and SH3 domain were duplicated from pINPOPH, [ 36 ] pET24(a)‐pd‐YFP [ 37 ] and pET‐Plac‐EC‐SH3d (an in‐house plasmid), respectively, and then assembled by overlap PCR amplification. The PCR product was further digested and inserted into pVLT33s (an in‐house plasmid, in which the kanamycin selection marker of pVLT33 was replaced with spectinomycin).…”
Section: Methodsmentioning
confidence: 99%
“…BET involves exposing solid materials to nitrogen gas under a variety of conditions and assessing the mass uptake. Furthermore, Augustin et al proposed a method to improve indirect SA measurement of silver NPs by specific biomolecules' affinity coordination [2]. The improved technique, however, is highly sensitive to the chemical composition of the NPs.…”
Section: Introductionmentioning
confidence: 99%