A high-sensitivity electrochemical immunosensor based on mobile crystalline material-41–polyvinyl alcohol nanocomposite and colloidal gold nanoparticles

Omidfar, Kobra; Zarei, Hajar; Gholizadeh, Fatemeh; Larijani, Bagher

doi:10.1016/j.ab.2011.12.022

Cited by 64 publications

(38 citation statements)

References 39 publications

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“…Many electrochemical immunosensor utilizing novel carrier systems have been developed for instance; carbon nanotube/manganese dioxide [47], graphene and Cu@Ag core-shell NPs [48], gold NPs [49]. Horseradish peroxidase and ferroferric oxide were used as a signal …”

Section: Electrochemical Immunosensormentioning

confidence: 99%

Recent Advances and Applications of Biosensors in Novel Technology

Rajpoot¹

2017

Biosens J

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In recent time, several novel biosensors such as enzyme based, immunosensors, tissue-based, DNA biosensors, piezoelectric, and thermal biosensors have been explored with high sensitivity by many research groups. These biosensors exhibit many essential benefits, including operational simplicity, remarkable sensitivity, low-cost instrumentation, the potential of automation, inherent miniaturization. They have offered elegant paths for the detection of even trace amounts of analytical agents of biological significance existing from macromolecules (e.g., disease biomarkers and antigens) and small molecules (e.g., natural toxins and haptens) to cells, viruses, and bacteria. Many electrochemical techniques viz., amperometry and voltammetry, photoelectrochemistry, electrochemiluminescence, impedance, piezoelectricity, potentiometry, alternating current electrohydrodynamics, and field-effect transistor have been utilized in the development of immunoassays to attain high sensitivity for electrochemical signal transduction. Recent developments in biological methods and instrumentation after using fluorescence tag to various nanocarriers such as nanoparticles, nanowires, nanotubes, etc. have improved the sensitivity of biosensors. Utilization of nucleotides/aptamers, affibodies, molecule imprinted polymers, and peptide arrays offer great tools to prepare advanced biosensors. Merging of nanotechnology with biosensor systems enhanced the diagnostic capability. This review gives an outline of the advances in biosensors technology relating biomedical sciences.

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Section: Electrochemical Immunosensormentioning

confidence: 99%

Recent Advances and Applications of Biosensors in Novel Technology

Rajpoot¹

2017

Biosens J

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“…PVP-capped CdS particles were used for haemoglobin electroanalysis, allowed the achievement of a 5 nM LOD (Liu et al, 2006). Omidfar et al (2012), developed the high sensitivity electrochemical HSA sensor based on HSA/AuNPs (electrochemical label), MCM-41/PVA (immobilised platform) was exhibited with 1 mM LOD and excellent stability. So far, protein and amino acids sensors have been intensively developed, although many improvements are required in reproducibility and sensitivity.…”

Section: Mnppf For Amino Acids and Proteins Detectionmentioning

confidence: 99%

Polymer thin films embedded with metal nanoparticles for electrochemical biosensors applications

Prakash

Chakrabarty

Singh

et al. 2013

Biosensors and Bioelectronics

181

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“…Gold nanoparticles (AuNPs) have been applied in immunosensors due to their high specific surface and the ability for immobilizing antibody [16]. Compared with conventional modification method, the electrodeposition of nanoparticles enables AuNPs more evenly and firmly to be deposited on the working electrode.…”

Section: Introductionmentioning

confidence: 99%

A sandwich electrochemical immunosensor for Salmonella pullorum and Salmonella gallinarum based on a screen-printed carbon electrode modified with an ionic liquid and electrodeposited gold nanoparticles

2015

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This article describes an electrochemical immunosensor for rapid determination of Salmonella pullorum and Salmonella gallinarum. The first step in the preparation of the immunosensor involves the electrodeposition of gold nanoparticles used for capturing antibody and enhancing signals. In order to generate a benign microenvironment for the antibody, the ionic liquid (IL) 1-butyl-3-methylimidazolium hexafluorophosphate was used to modify the surface of a screen-printed carbon electrode (SPCE). The single steps of modification were monitored via cyclic voltammetry and electrochemical impedance spectroscopy. Based on these findings, a sandwich immunoassay was worked out for the two Salmonella species by immobilizing the respective unlabeled antibodies on the SPCE. Following exposure to the analytes, secondary antibody (labeled with HRP) is added to form the sandwich. After adding hydrogen peroxide and thionine, the latter is oxidized and its signal measured via CV. A linear response to the Salmonella species is obtained in the 10 4 to 10 9 cfu·mL −1 concentration range, and the detection limits are 3.0×10 3 cfu·mL −1 for both species (at an SNR of 3). This assay is sensitive, highly specific, acceptably accurate and reproducible. Given its low detection limit, it represents a promising tool for the detection of S. pullorum, S. gallinarum, and -conceivably -of other food-borne pathogens by exchanging the antibody.

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A high-sensitivity electrochemical immunosensor based on mobile crystalline material-41–polyvinyl alcohol nanocomposite and colloidal gold nanoparticles

Cited by 64 publications

References 39 publications

Recent Advances and Applications of Biosensors in Novel Technology

Recent Advances and Applications of Biosensors in Novel Technology

Polymer thin films embedded with metal nanoparticles for electrochemical biosensors applications

A sandwich electrochemical immunosensor for Salmonella pullorum and Salmonella gallinarum based on a screen-printed carbon electrode modified with an ionic liquid and electrodeposited gold nanoparticles

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