A high sensitivity amperometric biosensor using a monomolecular layer of laccase as biorecognition element

Enzyme immobilization: an update

“…Enzymes for the most cases are immobilized either directly on a transducer's working tip or using a polymer membrane tightly wrapping it up. In principle, due to enzyme specificity and sensitivity, biosensors can be tailored for nearly any target analyte [91][92][93][94], and these can be both enzyme substrates and enzyme inhibitors. Advantageously, their determination is performed without special preparation of the sample.…”

Hybrid Film Biosensor for Phenolic Compounds Detection

“…They include the modification of solid graphite (Ortega et al, 1994), incorporation of enzyme into carbon paste, immobilization on surface of different membranes (Yarpolov et www.intechopen.com al., 1995), Langmuir-Blodgett hybrid films (Cabaj et al, 2010a;Cabaj et al, 2008;. The most sensitive biosensors are based on tyrosinases (Psrellada et al, 1997), however, in order to low stability of this class of enzymes, these devices usually present short life-times (Vianello et al, 2004). Alternatively to tyrosinases -laccases are often used.…”

“…The immobilization methods for laccase or tyrosinase such as physical adsorption (Cabaj et al, 2010a;Cabaj et al, 2010b), covalent attachment (Vianello eta al., 2004), incorporation within carbon paste (Duran et al, 2002), immobilization in polymer films (Timur et al, 2004), entrapment in some sol-gel matrices (Duran et al, 2002) have been also reported in the literature. Vianello et al (2004) presented a high-sensitivity flow biosensor based on a monomolecular layer of laccase immobilized on a gold support. This biosensor detects phenols in the low micromolar range, i.e.…”

White rot fungi 4222 degrade lignocellulose of corn straw

“…[1] [2] White rot fungi is effective in the degradation of plant fiber raw material lignin [3][4], is currently the strongest known ability to degrade lignin in nature, can only under the condition of pure culture lignin will eventually reach the mineralization type of microorganisms. Using the characteristic of the white-rot fungus, has been in the biological pulping, bio bleaching, bleaching effluent, biochemical treatment, and so on [4], some of which have been used for test phase intermediate production, showed a good application prospect [5].…”