A high-resolution radiation hybrid map of the proximal region of rat Chromosome 4

Al-Majali, Khulood M.; Glazier, Anne M.; Norsworthy, Penny J.; Wahid, Faisal; Cooper, Lisa D.; Wallace, Caroline A.; Scott, James A.; Lausen, Berthold; Aitman, Timothy J.

doi:10.1007/s003359901025

Cited by 5 publications

(1 citation statement)

References 27 publications

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“…Control hamster primers derived from the hamster sequence of the Dhfr gene were used in each PCR reaction together with the rat-specific primers, to give a hamster-specific band in all PCR reactions [16]. PCR products for Col4a3(1) and Col4a3(2) primer pairs were assessed from the RH panel of 106 hybrid DNAs and graded as positive or negative.…”

Section: Methodsmentioning

confidence: 99%

Segregation of Experimental Autoimmune Glomerulonephritis as a Complex Genetic Trait and Exclusion of <i>Col4a3</i> as a Candidate Gene

Reynolds

Cook

Ryan

et al. 2002

Nephron Exp Nephrol

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Experimental autoimmune glomerulonephritis (EAG), an animal model of Goodpasture’s disease, can be induced in Wistar-Kyoto (WKY) rats (RT1-l) by immunization with rat glomerular basement membrane (GBM) in adjuvant. The model in this rat strain is characterized by anti-GBM antibody production accompanied by focal necrotizing glomerulonephritis with crescent formation. The main autoantigen in humans and rats has been identified as the non-collagenous domain of the α3 chain of type IV collagen (α3(IV)NC1). By contrast, Lewis (LEW) rats with the same MHC background (RT1-l), immunized with the same antigen, develop similar levels of circulating anti-GBM antibodies, but no histological evidence of nephritis. In order to investigate the genetic basis of susceptibility to EAG, we examined the response of both F1 (WKY × LEW) and backcross (BC1; WKY × F1) rats to immunization with rat GBM. F1 animals were completely resistant to the development of EAG, while BC1 animals showed a range of responses from severe crescentic glomerulonephritis to no histological evidence of disease. The results indicate that EAG is inherited as a complex trait under the control of WKY genes unlinked to the MHC. cDNA sequence analysis of α3(IV)NC1 in the two parental strains was identical, indicating no predicted amino acid sequence variation in the α3(IV)NC1 domain between these strains. Radiation hybrid mapping, using two separate PCR amplicons from rat α3(IV)NC1, localized rat Col4a3 to a region of chromosome 9. Since Col4a3 (encoding the autoantigen) is a candidate for susceptibility to EAG, we screened the region of rat chromosome 9 where Col4a3 is localized, using polymorphic microsatellite markers in segregating BC1 progeny. No significant linkage was detected. These results exclude Col4a3 as a recessive susceptibility gene for EAG in the BC1 progeny.

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Section: Methodsmentioning

confidence: 99%

Segregation of Experimental Autoimmune Glomerulonephritis as a Complex Genetic Trait and Exclusion of <i>Col4a3</i> as a Candidate Gene

Reynolds

Cook

Ryan

et al. 2002

Nephron Exp Nephrol

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The Functional Significance of Absence: The Chromosomal Segment Harboring Tp53 Is Absent from the T55 Rat Radiation Hybrid Mapping Panel

et al. 2002

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Integrative Genomics: In Silico Coupling of Rat Physiology and Complex Traits With Mouse and Human Data

Twigger¹,

Nie²,

Ruotti³

et al. 2004

Genome Res.

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Integration of the large variety of genome maps from several organisms provides the mechanism by which physiological knowledge obtained in model systems such as the rat can be projected onto the human genome to further the research on human disease. The release of the rat genome sequence provides new information for studies using the rat model and is a key reference against which existing and new rat physiological results can be aligned. Previously, we described comparative maps of the rat, mouse, and human based on EST sequence comparisons combined with radiation hybrid maps. Here, we use new data and introduce the Integrated Genomics Environment, an extensive database of curated and integrated maps, markers, and physiological results. These results are integrated by using VCMapview, a java-based map integration and visualization tool. This unique environment allows researchers to relate results from cytogenetic, genetic, and radiation hybrid studies to the genome sequence and compare regions of interest between human, mouse, and rat. Integrating rat physiology with mouse genetics and clinical results from human by using the respective genomes provides a novel route to capitalize on comparative genomics and the strengths of model organism biology.

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A high-resolution radiation hybrid map of the proximal region of rat Chromosome 4

Cited by 5 publications

References 27 publications

Segregation of Experimental Autoimmune Glomerulonephritis as a Complex Genetic Trait and Exclusion of <i>Col4a3</i> as a Candidate Gene

Segregation of Experimental Autoimmune Glomerulonephritis as a Complex Genetic Trait and Exclusion of <i>Col4a3</i> as a Candidate Gene

The Functional Significance of Absence: The Chromosomal Segment Harboring Tp53 Is Absent from the T55 Rat Radiation Hybrid Mapping Panel

Integrative Genomics: In Silico Coupling of Rat Physiology and Complex Traits With Mouse and Human Data

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